SlideShare a Scribd company logo
Presented by
Dr. Sapnil, MDS
Reader
PRINCIPLES OF DIAGNOSIS
SPECIFICITY
Ability of test or observation to differentiate one
disease to another
SENSITIVITY
Ability of test or observation to detect the disease
whenever it is present
I CLINICAL DIAGNOSIS
II RADIOGRAPHIC ASSESSMENT
III MICROBIOLOGIC ANALYSIS
IV IMMUNOLOGICAL DIAGNOSIS
V BIOCHEMICAL METHODS
I CLINICAL DIAGNOSIS
Based on signs of gingival inflammation
A CHANGE IN COLOR
B GINGIVAL BLEEDING
C INCREASE FLOW OF GINGIVAL FLUID
D INCREASE TEMPERATURE OF GINGIVAL TISSUES
A CHANGE IN COLOR
NO DIAGNOSTIC TESTS
SUBJECTIVE ESTIMATION
B GINGIVAL BLEEDING
1. PERIODONTAL PROBES
a. CONVENTIONAL PROBES
b. ADVANCED PROBES
2. WOODEN TOOTH PICKS
3. UNWAXED DENTAL FLOSS
a. CONVENTIONAL PROBES
WHO / CPITN probe
WILLIAM’S probe
UNIVERSITY OF MICHIGAN “O” probe
UNC-15 probe
MARQUIS COLOR-CODED probe
LIMITATIONS IN CONVENTIONAL PROBES
ANGULATION PRESSURE
b. ADVANCED PROBES
PRESSURE SENSITIVE PROBE - 30GRAMS PRESSURE
ELECTRONIC PERIODONTAL PROBE - 25 GRAMS PRESSURE
AUTOMATED PERIODONTAL PROBE - TIP—0.5MM
COMPUTERIZED PERIODONTAL PROBE [FLORIDA]
- TIP—0.4MM
JEFFCOAT PROBE [FOSTER-MILLER ]
INTERPROBE
BIREK PROBE
2. WOODEN TOOTH PICKS
INTERDENTAL BLEEDING INDEX - CATON & PALSON
3.UNWAXED FLOSS
GINGIVAL BLEEDING INDEX - CARTER & BARNESS
C INCREASE FLOW OF GINGIVAL FLUID
1. MANUAL METHOD
NINHYDRIN PAPER -- 1.5mm - 1mm introduced into
the sulcus - for 3mnts - 0.1mg of fluid is collected
2. ELECTRONIC
PERIOTRON 2000 and 6000
D INCREASE TEMPERATURE OF GINGIVAL TISSUES
THERMOCOUPLE
PERIOTEMP
II RADIOGRAPHIC ASSESSMENT [BONE DESTRUCTION/LOSS]
OPG
IOPA
XERORADIOGRAPHY
I125 ABSORPTIOMETRY
PHOTO-DENSITOMETRIC ANALYSIS TECH
COMPUTER ASSISTED SUBSTRACTION RADIOGRAPHY
COMPUTER ASSISTED DENSITOMETRIC IMAGE ANALYSIS
NUCLEAR MEDICINE
LIMITATIONS IN CONVENTIONAL PERIAPICAL RADIOGRAPHY
RADIOGRAPHIC PARALLELING TECHNIQUE
RADIOGRAPHIC PARELLELING TECHNIQUE
FOR
ASSESSMENT OF ALVEOLAR BONE CHANGES
Occlusal imprint in film holder individual film holder in paralleling device
Individualized paralleling technique in place intra orally
Two radiographs obtained 6months apart showing identical projection geometry
DIGITAL SUBSTRACTION RADIOGRAPHY
Serial radiographs – digital images – superimposed – video screen
Changes in the density/volume of bone can be detected as
lighter areas (bone gain) or dark areas (bone loss)
III MICROBIOLOGICAL ANALYSIS
1. DARK FIELD /PHASE CONTRAST MICROSCOPY
2. IMMUNO FLORESCENCE MICROSCOPY
a. direct
b. indirect
3. LATEX AGGLUTINATION
4. FLOW CYTOMETRY
5. ELISA
6. BANA[ BENZYL ARGENINE NAPTHALAMIDE ]
7. DNA PROBE[Aa, Pg, Pi, Td ]
a. genomic
b. oligonucleotide
c. cloned probe
MICROBIAL SAMPLING OF THE SUBGINGIVAL FLORA
SAMPLING METHOD FOR CREVICULAR FLUID ANALYSIS
PERIOTRON SYSTEM
DNA PROBE
Based on the ability of DNA to hybridize or bind
to complimentary strands of DNA or RNA having
the same base
sequence
The plaque sample is collected and sent to the lab
A portion of DNA from specific bacterium is
isolated with the aid of enzyme called as
Restriction Endonucleoses [RE] that cut the DNA
molecule at specific location into single strands
These single strands are the probe for
complimentary sequences of specific bacterium
This single stranded bacterial DNA is inoculated
on a Nitrocellulose membrane
The specific labeled DNA probe is inoculated on
the membrane to allow hybridization for 15mnts
If hybridisation of 2 single strands takes place to
form a double helix, the test is + ve, if not - ve
DNA PROBES available for Aa, Pg, Pi and Td
IV IMMUNOLOGICAL DIAGNOSIS
Identification of defective chemotactic and
phagocytic functions of PMNL / monocytes in
diseases such as
LJP
PREPUBERTAL
RPP
ANUG
by blood examination
V BIOCHEMICAL
enzymes present in severe periodontitis such as
PPP, LJP, RPP are
Lysozymes
Collagenase
Alkaline phosphate/acid
Beta glucoronidase
Cathepsin - D
Elastase
Arlysulphatase
Prostaglandins
Myloperoxidase[MPO]
identified by tests such as
PERIOSCAN
PROGNOSTIC
PERIOGAURD
PERIOCHECK

More Related Content

Similar to ADVANCED DIAGNOSTIC TECHNIQUES.ppt

Vitreous biopsy in_the_diagnosis_of_uveitis
Vitreous biopsy in_the_diagnosis_of_uveitisVitreous biopsy in_the_diagnosis_of_uveitis
Vitreous biopsy in_the_diagnosis_of_uveitis
Abada Fida
 
Tuberculosis diagnostics
Tuberculosis diagnosticsTuberculosis diagnostics
Tuberculosis diagnostics
Santi Silairatana
 
Advanced diagnostic aids in Periodontics
Advanced diagnostic aids in PeriodonticsAdvanced diagnostic aids in Periodontics
Advanced diagnostic aids in Periodontics
R Viswa Chandra
 
LaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptxLaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptx
MANJUSINGH948460
 
Biopsy
BiopsyBiopsy
Biopsy
Ajish Saji
 
Advances in Microbiological diagnosis of Periodontal diseases
Advances in Microbiological diagnosis of Periodontal diseasesAdvances in Microbiological diagnosis of Periodontal diseases
Advances in Microbiological diagnosis of Periodontal diseases
Dr Fariya Ashraf
 
Desquamative gingivitis
Desquamative gingivitisDesquamative gingivitis
Desquamative gingivitis
mikitha p
 
Diagnosis of tuberculosis
Diagnosis of tuberculosisDiagnosis of tuberculosis
Diagnosis of tuberculosis
Khaled ezzat
 
VIROLOGY - DIAGNOSTIC METHODS
VIROLOGY - DIAGNOSTIC METHODSVIROLOGY - DIAGNOSTIC METHODS
VIROLOGY - DIAGNOSTIC METHODS
Meenatchisundaram Subramani
 
Candidiasis jc
Candidiasis jcCandidiasis jc
Candidiasis jc
priyadershini rangari
 
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptxADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
veena621629
 
Seminario BIOMOL - A novel polymerase…
Seminario BIOMOL - A novel polymerase…Seminario BIOMOL - A novel polymerase…
Seminario BIOMOL - A novel polymerase…
XimenaConsuegraRuiz1
 
The Covid-19 Pathway
The Covid-19 PathwayThe Covid-19 Pathway
The Covid-19 Pathway
Shervin Molayem, DDS
 
Human genome project
Human genome projectHuman genome project
Human genome project
Sachin Ekatpure
 
Microarray
Microarray Microarray
Microarray
microbiologiist
 
Salivary markers of systemic diseases ppt
Salivary markers of systemic diseases pptSalivary markers of systemic diseases ppt
Salivary markers of systemic diseases ppt
pharmacologyseminars
 
Proof of concept of WGS based surveillance: meningococcal disease
Proof of concept of WGS based surveillance: meningococcal diseaseProof of concept of WGS based surveillance: meningococcal disease
Proof of concept of WGS based surveillance: meningococcal disease
European Centre for Disease Prevention and Control (ECDC)
 
8. Breast Cancer Diagnostic Workup .pptx
8. Breast Cancer Diagnostic Workup .pptx8. Breast Cancer Diagnostic Workup .pptx
8. Breast Cancer Diagnostic Workup .pptx
AbhishekMewara2
 
Overcoming the challenges of molecular diagnostics in government health insti...
Overcoming the challenges of molecular diagnostics in government health insti...Overcoming the challenges of molecular diagnostics in government health insti...
Overcoming the challenges of molecular diagnostics in government health insti...
Yakubu Sunday Bot
 
LaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptxLaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptx
Kanishka478113
 

Similar to ADVANCED DIAGNOSTIC TECHNIQUES.ppt (20)

Vitreous biopsy in_the_diagnosis_of_uveitis
Vitreous biopsy in_the_diagnosis_of_uveitisVitreous biopsy in_the_diagnosis_of_uveitis
Vitreous biopsy in_the_diagnosis_of_uveitis
 
Tuberculosis diagnostics
Tuberculosis diagnosticsTuberculosis diagnostics
Tuberculosis diagnostics
 
Advanced diagnostic aids in Periodontics
Advanced diagnostic aids in PeriodonticsAdvanced diagnostic aids in Periodontics
Advanced diagnostic aids in Periodontics
 
LaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptxLaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptx
 
Biopsy
BiopsyBiopsy
Biopsy
 
Advances in Microbiological diagnosis of Periodontal diseases
Advances in Microbiological diagnosis of Periodontal diseasesAdvances in Microbiological diagnosis of Periodontal diseases
Advances in Microbiological diagnosis of Periodontal diseases
 
Desquamative gingivitis
Desquamative gingivitisDesquamative gingivitis
Desquamative gingivitis
 
Diagnosis of tuberculosis
Diagnosis of tuberculosisDiagnosis of tuberculosis
Diagnosis of tuberculosis
 
VIROLOGY - DIAGNOSTIC METHODS
VIROLOGY - DIAGNOSTIC METHODSVIROLOGY - DIAGNOSTIC METHODS
VIROLOGY - DIAGNOSTIC METHODS
 
Candidiasis jc
Candidiasis jcCandidiasis jc
Candidiasis jc
 
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptxADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
ADVANCED CLINICAL AND RADIOGRAPHIC DIAGNOSTIC AIDS (2).pptx
 
Seminario BIOMOL - A novel polymerase…
Seminario BIOMOL - A novel polymerase…Seminario BIOMOL - A novel polymerase…
Seminario BIOMOL - A novel polymerase…
 
The Covid-19 Pathway
The Covid-19 PathwayThe Covid-19 Pathway
The Covid-19 Pathway
 
Human genome project
Human genome projectHuman genome project
Human genome project
 
Microarray
Microarray Microarray
Microarray
 
Salivary markers of systemic diseases ppt
Salivary markers of systemic diseases pptSalivary markers of systemic diseases ppt
Salivary markers of systemic diseases ppt
 
Proof of concept of WGS based surveillance: meningococcal disease
Proof of concept of WGS based surveillance: meningococcal diseaseProof of concept of WGS based surveillance: meningococcal disease
Proof of concept of WGS based surveillance: meningococcal disease
 
8. Breast Cancer Diagnostic Workup .pptx
8. Breast Cancer Diagnostic Workup .pptx8. Breast Cancer Diagnostic Workup .pptx
8. Breast Cancer Diagnostic Workup .pptx
 
Overcoming the challenges of molecular diagnostics in government health insti...
Overcoming the challenges of molecular diagnostics in government health insti...Overcoming the challenges of molecular diagnostics in government health insti...
Overcoming the challenges of molecular diagnostics in government health insti...
 
LaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptxLaboratorydiagnosisofTB.pptx
LaboratorydiagnosisofTB.pptx
 

More from Department of periodontics S B Patil Institute for Dental Sciences & Research

RESECTIVE OSSEOUS SURGERY.ppt
RESECTIVE OSSEOUS SURGERY.pptRESECTIVE OSSEOUS SURGERY.ppt
REGENERATIVE OSSEOUS SURGERY.ppt
REGENERATIVE OSSEOUS SURGERY.pptREGENERATIVE OSSEOUS SURGERY.ppt
PLASTIC AND ESTHETIC SURGERY.ppt
PLASTIC AND ESTHETIC SURGERY.pptPLASTIC AND ESTHETIC SURGERY.ppt
FURCATION MANAGEMENT.ppt
FURCATION  MANAGEMENT.pptFURCATION  MANAGEMENT.ppt
FLAP TECHNIQUE.ppt
FLAP TECHNIQUE.pptFLAP TECHNIQUE.ppt
THE PERIODONTAL FLAP.ppt
THE PERIODONTAL FLAP.pptTHE PERIODONTAL FLAP.ppt
PERIODONTAL TREATMENT FOR OLDER ADULTS.ppt
PERIODONTAL TREATMENT FOR OLDER ADULTS.pptPERIODONTAL TREATMENT FOR OLDER ADULTS.ppt
PERIO - PULPAL RELATION.ppt
PERIO - PULPAL RELATION.pptPERIO - PULPAL RELATION.ppt
ADVANCES IN SURGICAL TECHNOLOGY.ppt
ADVANCES IN SURGICAL TECHNOLOGY.pptADVANCES IN SURGICAL TECHNOLOGY.ppt
EPIDEMIOLOGY.ppt
EPIDEMIOLOGY.pptEPIDEMIOLOGY.ppt
CASE HISTORY.ppt
CASE HISTORY.pptCASE HISTORY.ppt
BONE LOSS AND PATTERNS OF BONE DESTRUCTION.ppt
BONE LOSS AND PATTERNS OF BONE DESTRUCTION.pptBONE LOSS AND PATTERNS OF BONE DESTRUCTION.ppt
AIDS AND PERIODONTIUM.ppt
AIDS AND PERIODONTIUM.pptAIDS AND PERIODONTIUM.ppt
RATIONALE FOR PERIODONTAL TREATMENT.ppt
RATIONALE FOR PERIODONTAL TREATMENT.pptRATIONALE FOR PERIODONTAL TREATMENT.ppt
DESQUAMATIVE GINGIVITIS.ppt
DESQUAMATIVE GINGIVITIS.pptDESQUAMATIVE GINGIVITIS.ppt
AGING AND THE PERIODONTIUM.ppt
AGING AND THE PERIODONTIUM.pptAGING AND THE PERIODONTIUM.ppt
AGGRESSIVE PERIODONTITIS.ppt
AGGRESSIVE PERIODONTITIS.pptAGGRESSIVE PERIODONTITIS.ppt
THE PERIODONTAL POCKET.ppt
THE PERIODONTAL POCKET.pptTHE PERIODONTAL POCKET.ppt
THE PERIODONTAL INSTRUMENTARIUM.ppt
THE PERIODONTAL INSTRUMENTARIUM.pptTHE PERIODONTAL INSTRUMENTARIUM.ppt
THE GINGIVA.ppt
THE GINGIVA.pptTHE GINGIVA.ppt

More from Department of periodontics S B Patil Institute for Dental Sciences & Research (20)

RESECTIVE OSSEOUS SURGERY.ppt
RESECTIVE OSSEOUS SURGERY.pptRESECTIVE OSSEOUS SURGERY.ppt
RESECTIVE OSSEOUS SURGERY.ppt
 
REGENERATIVE OSSEOUS SURGERY.ppt
REGENERATIVE OSSEOUS SURGERY.pptREGENERATIVE OSSEOUS SURGERY.ppt
REGENERATIVE OSSEOUS SURGERY.ppt
 
PLASTIC AND ESTHETIC SURGERY.ppt
PLASTIC AND ESTHETIC SURGERY.pptPLASTIC AND ESTHETIC SURGERY.ppt
PLASTIC AND ESTHETIC SURGERY.ppt
 
FURCATION MANAGEMENT.ppt
FURCATION  MANAGEMENT.pptFURCATION  MANAGEMENT.ppt
FURCATION MANAGEMENT.ppt
 
FLAP TECHNIQUE.ppt
FLAP TECHNIQUE.pptFLAP TECHNIQUE.ppt
FLAP TECHNIQUE.ppt
 
THE PERIODONTAL FLAP.ppt
THE PERIODONTAL FLAP.pptTHE PERIODONTAL FLAP.ppt
THE PERIODONTAL FLAP.ppt
 
PERIODONTAL TREATMENT FOR OLDER ADULTS.ppt
PERIODONTAL TREATMENT FOR OLDER ADULTS.pptPERIODONTAL TREATMENT FOR OLDER ADULTS.ppt
PERIODONTAL TREATMENT FOR OLDER ADULTS.ppt
 
PERIO - PULPAL RELATION.ppt
PERIO - PULPAL RELATION.pptPERIO - PULPAL RELATION.ppt
PERIO - PULPAL RELATION.ppt
 
ADVANCES IN SURGICAL TECHNOLOGY.ppt
ADVANCES IN SURGICAL TECHNOLOGY.pptADVANCES IN SURGICAL TECHNOLOGY.ppt
ADVANCES IN SURGICAL TECHNOLOGY.ppt
 
EPIDEMIOLOGY.ppt
EPIDEMIOLOGY.pptEPIDEMIOLOGY.ppt
EPIDEMIOLOGY.ppt
 
CASE HISTORY.ppt
CASE HISTORY.pptCASE HISTORY.ppt
CASE HISTORY.ppt
 
BONE LOSS AND PATTERNS OF BONE DESTRUCTION.ppt
BONE LOSS AND PATTERNS OF BONE DESTRUCTION.pptBONE LOSS AND PATTERNS OF BONE DESTRUCTION.ppt
BONE LOSS AND PATTERNS OF BONE DESTRUCTION.ppt
 
AIDS AND PERIODONTIUM.ppt
AIDS AND PERIODONTIUM.pptAIDS AND PERIODONTIUM.ppt
AIDS AND PERIODONTIUM.ppt
 
RATIONALE FOR PERIODONTAL TREATMENT.ppt
RATIONALE FOR PERIODONTAL TREATMENT.pptRATIONALE FOR PERIODONTAL TREATMENT.ppt
RATIONALE FOR PERIODONTAL TREATMENT.ppt
 
DESQUAMATIVE GINGIVITIS.ppt
DESQUAMATIVE GINGIVITIS.pptDESQUAMATIVE GINGIVITIS.ppt
DESQUAMATIVE GINGIVITIS.ppt
 
AGING AND THE PERIODONTIUM.ppt
AGING AND THE PERIODONTIUM.pptAGING AND THE PERIODONTIUM.ppt
AGING AND THE PERIODONTIUM.ppt
 
AGGRESSIVE PERIODONTITIS.ppt
AGGRESSIVE PERIODONTITIS.pptAGGRESSIVE PERIODONTITIS.ppt
AGGRESSIVE PERIODONTITIS.ppt
 
THE PERIODONTAL POCKET.ppt
THE PERIODONTAL POCKET.pptTHE PERIODONTAL POCKET.ppt
THE PERIODONTAL POCKET.ppt
 
THE PERIODONTAL INSTRUMENTARIUM.ppt
THE PERIODONTAL INSTRUMENTARIUM.pptTHE PERIODONTAL INSTRUMENTARIUM.ppt
THE PERIODONTAL INSTRUMENTARIUM.ppt
 
THE GINGIVA.ppt
THE GINGIVA.pptTHE GINGIVA.ppt
THE GINGIVA.ppt
 

Recently uploaded

বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdfবাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
eBook.com.bd (প্রয়োজনীয় বাংলা বই)
 
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skillsspot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
haiqairshad
 
clinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdfclinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdf
Priyankaranawat4
 
Liberal Approach to the Study of Indian Politics.pdf
Liberal Approach to the Study of Indian Politics.pdfLiberal Approach to the Study of Indian Politics.pdf
Liberal Approach to the Study of Indian Politics.pdf
WaniBasim
 
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPLAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
RAHUL
 
Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...
PsychoTech Services
 
A Independência da América Espanhola LAPBOOK.pdf
A Independência da América Espanhola LAPBOOK.pdfA Independência da América Espanhola LAPBOOK.pdf
A Independência da América Espanhola LAPBOOK.pdf
Jean Carlos Nunes Paixão
 
How to Make a Field Mandatory in Odoo 17
How to Make a Field Mandatory in Odoo 17How to Make a Field Mandatory in Odoo 17
How to Make a Field Mandatory in Odoo 17
Celine George
 
MARY JANE WILSON, A “BOA MÃE” .
MARY JANE WILSON, A “BOA MÃE”           .MARY JANE WILSON, A “BOA MÃE”           .
MARY JANE WILSON, A “BOA MÃE” .
Colégio Santa Teresinha
 
How to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 InventoryHow to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 Inventory
Celine George
 
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxBeyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
EduSkills OECD
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
Katrina Pritchard
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
Krassimira Luka
 
How to Create a More Engaging and Human Online Learning Experience
How to Create a More Engaging and Human Online Learning Experience How to Create a More Engaging and Human Online Learning Experience
How to Create a More Engaging and Human Online Learning Experience
Wahiba Chair Training & Consulting
 
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
Nguyen Thanh Tu Collection
 
Main Java[All of the Base Concepts}.docx
Main Java[All of the Base Concepts}.docxMain Java[All of the Base Concepts}.docx
Main Java[All of the Base Concepts}.docx
adhitya5119
 
Solutons Maths Escape Room Spatial .pptx
Solutons Maths Escape Room Spatial .pptxSolutons Maths Escape Room Spatial .pptx
Solutons Maths Escape Room Spatial .pptx
spdendr
 
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
สมใจ จันสุกสี
 
Your Skill Boost Masterclass: Strategies for Effective Upskilling
Your Skill Boost Masterclass: Strategies for Effective UpskillingYour Skill Boost Masterclass: Strategies for Effective Upskilling
Your Skill Boost Masterclass: Strategies for Effective Upskilling
Excellence Foundation for South Sudan
 
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptxPengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Fajar Baskoro
 

Recently uploaded (20)

বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdfবাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
 
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skillsspot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
 
clinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdfclinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdf
 
Liberal Approach to the Study of Indian Politics.pdf
Liberal Approach to the Study of Indian Politics.pdfLiberal Approach to the Study of Indian Politics.pdf
Liberal Approach to the Study of Indian Politics.pdf
 
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPLAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
 
Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...
 
A Independência da América Espanhola LAPBOOK.pdf
A Independência da América Espanhola LAPBOOK.pdfA Independência da América Espanhola LAPBOOK.pdf
A Independência da América Espanhola LAPBOOK.pdf
 
How to Make a Field Mandatory in Odoo 17
How to Make a Field Mandatory in Odoo 17How to Make a Field Mandatory in Odoo 17
How to Make a Field Mandatory in Odoo 17
 
MARY JANE WILSON, A “BOA MÃE” .
MARY JANE WILSON, A “BOA MÃE”           .MARY JANE WILSON, A “BOA MÃE”           .
MARY JANE WILSON, A “BOA MÃE” .
 
How to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 InventoryHow to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 Inventory
 
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxBeyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptx
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
 
How to Create a More Engaging and Human Online Learning Experience
How to Create a More Engaging and Human Online Learning Experience How to Create a More Engaging and Human Online Learning Experience
How to Create a More Engaging and Human Online Learning Experience
 
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
 
Main Java[All of the Base Concepts}.docx
Main Java[All of the Base Concepts}.docxMain Java[All of the Base Concepts}.docx
Main Java[All of the Base Concepts}.docx
 
Solutons Maths Escape Room Spatial .pptx
Solutons Maths Escape Room Spatial .pptxSolutons Maths Escape Room Spatial .pptx
Solutons Maths Escape Room Spatial .pptx
 
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
คำศัพท์ คำพื้นฐานการอ่าน ภาษาอังกฤษ ระดับชั้น ม.1
 
Your Skill Boost Masterclass: Strategies for Effective Upskilling
Your Skill Boost Masterclass: Strategies for Effective UpskillingYour Skill Boost Masterclass: Strategies for Effective Upskilling
Your Skill Boost Masterclass: Strategies for Effective Upskilling
 
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptxPengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
 

ADVANCED DIAGNOSTIC TECHNIQUES.ppt

  • 2. PRINCIPLES OF DIAGNOSIS SPECIFICITY Ability of test or observation to differentiate one disease to another SENSITIVITY Ability of test or observation to detect the disease whenever it is present
  • 3. I CLINICAL DIAGNOSIS II RADIOGRAPHIC ASSESSMENT III MICROBIOLOGIC ANALYSIS IV IMMUNOLOGICAL DIAGNOSIS V BIOCHEMICAL METHODS
  • 4. I CLINICAL DIAGNOSIS Based on signs of gingival inflammation A CHANGE IN COLOR B GINGIVAL BLEEDING C INCREASE FLOW OF GINGIVAL FLUID D INCREASE TEMPERATURE OF GINGIVAL TISSUES
  • 5. A CHANGE IN COLOR NO DIAGNOSTIC TESTS SUBJECTIVE ESTIMATION
  • 6. B GINGIVAL BLEEDING 1. PERIODONTAL PROBES a. CONVENTIONAL PROBES b. ADVANCED PROBES 2. WOODEN TOOTH PICKS 3. UNWAXED DENTAL FLOSS
  • 7. a. CONVENTIONAL PROBES WHO / CPITN probe WILLIAM’S probe UNIVERSITY OF MICHIGAN “O” probe UNC-15 probe MARQUIS COLOR-CODED probe
  • 8. LIMITATIONS IN CONVENTIONAL PROBES ANGULATION PRESSURE
  • 9. b. ADVANCED PROBES PRESSURE SENSITIVE PROBE - 30GRAMS PRESSURE ELECTRONIC PERIODONTAL PROBE - 25 GRAMS PRESSURE AUTOMATED PERIODONTAL PROBE - TIP—0.5MM COMPUTERIZED PERIODONTAL PROBE [FLORIDA] - TIP—0.4MM JEFFCOAT PROBE [FOSTER-MILLER ] INTERPROBE BIREK PROBE
  • 10. 2. WOODEN TOOTH PICKS INTERDENTAL BLEEDING INDEX - CATON & PALSON
  • 11. 3.UNWAXED FLOSS GINGIVAL BLEEDING INDEX - CARTER & BARNESS
  • 12. C INCREASE FLOW OF GINGIVAL FLUID 1. MANUAL METHOD NINHYDRIN PAPER -- 1.5mm - 1mm introduced into the sulcus - for 3mnts - 0.1mg of fluid is collected 2. ELECTRONIC PERIOTRON 2000 and 6000
  • 13. D INCREASE TEMPERATURE OF GINGIVAL TISSUES THERMOCOUPLE PERIOTEMP
  • 14. II RADIOGRAPHIC ASSESSMENT [BONE DESTRUCTION/LOSS] OPG IOPA XERORADIOGRAPHY I125 ABSORPTIOMETRY PHOTO-DENSITOMETRIC ANALYSIS TECH COMPUTER ASSISTED SUBSTRACTION RADIOGRAPHY COMPUTER ASSISTED DENSITOMETRIC IMAGE ANALYSIS NUCLEAR MEDICINE
  • 15. LIMITATIONS IN CONVENTIONAL PERIAPICAL RADIOGRAPHY
  • 17. RADIOGRAPHIC PARELLELING TECHNIQUE FOR ASSESSMENT OF ALVEOLAR BONE CHANGES Occlusal imprint in film holder individual film holder in paralleling device Individualized paralleling technique in place intra orally Two radiographs obtained 6months apart showing identical projection geometry
  • 18. DIGITAL SUBSTRACTION RADIOGRAPHY Serial radiographs – digital images – superimposed – video screen Changes in the density/volume of bone can be detected as lighter areas (bone gain) or dark areas (bone loss)
  • 19. III MICROBIOLOGICAL ANALYSIS 1. DARK FIELD /PHASE CONTRAST MICROSCOPY 2. IMMUNO FLORESCENCE MICROSCOPY a. direct b. indirect 3. LATEX AGGLUTINATION 4. FLOW CYTOMETRY 5. ELISA 6. BANA[ BENZYL ARGENINE NAPTHALAMIDE ] 7. DNA PROBE[Aa, Pg, Pi, Td ] a. genomic b. oligonucleotide c. cloned probe
  • 20. MICROBIAL SAMPLING OF THE SUBGINGIVAL FLORA
  • 21. SAMPLING METHOD FOR CREVICULAR FLUID ANALYSIS PERIOTRON SYSTEM
  • 22. DNA PROBE Based on the ability of DNA to hybridize or bind to complimentary strands of DNA or RNA having the same base sequence The plaque sample is collected and sent to the lab
  • 23. A portion of DNA from specific bacterium is isolated with the aid of enzyme called as Restriction Endonucleoses [RE] that cut the DNA molecule at specific location into single strands These single strands are the probe for complimentary sequences of specific bacterium
  • 24. This single stranded bacterial DNA is inoculated on a Nitrocellulose membrane The specific labeled DNA probe is inoculated on the membrane to allow hybridization for 15mnts If hybridisation of 2 single strands takes place to form a double helix, the test is + ve, if not - ve DNA PROBES available for Aa, Pg, Pi and Td
  • 25. IV IMMUNOLOGICAL DIAGNOSIS Identification of defective chemotactic and phagocytic functions of PMNL / monocytes in diseases such as LJP PREPUBERTAL RPP ANUG by blood examination
  • 26. V BIOCHEMICAL enzymes present in severe periodontitis such as PPP, LJP, RPP are Lysozymes Collagenase Alkaline phosphate/acid Beta glucoronidase Cathepsin - D Elastase Arlysulphatase Prostaglandins Myloperoxidase[MPO]
  • 27. identified by tests such as PERIOSCAN PROGNOSTIC PERIOGAURD PERIOCHECK