This document discusses various principles and methods for diagnosing periodontal disease. It covers: 1. Clinical diagnosis based on signs like changes in gingival color, bleeding, and increased fluid/temperature. Methods include probes, dental floss, and thermocouples. 2. Radiographic assessment of bone destruction using techniques like X-rays and subtraction radiography. 3. Microbiological analysis of the subgingival flora using microscopy, latex agglutination, DNA probes to identify bacteria. 4. Immunological diagnosis of defective immune functions. 5. Biochemical identification of enzymes associated with severe periodontitis, measured by tests like Perioscan.