Get to Know About the Molecular Cytogenetic Analysis for Acute Lymphoblastic Leukemia. This test will diagnose and monitor the treatment response of patients.
Chronic myelogenous leukemia (CML) - pluripotential stem cell disease
A malignancy the treatment of which has been revolutionised over the last decade.
Here is a comprehensive discussion on the disease
Chronic myelogenous leukemia (CML) - pluripotential stem cell disease
A malignancy the treatment of which has been revolutionised over the last decade.
Here is a comprehensive discussion on the disease
Disease of older males.
The Philadelphia chromosome - BCR-ABL gene and it’s Tyrosine kinase protein – central to the pathogenesis.
Occurs in 3 phases
Imatinib has revolutionized the management
Roundtable Expert Discussions on CML Clinical Debates: A Collaborative Video Viewpoint Series With Medscape
This video viewpoint in its original and unaltered format is for educational purposes and is current as of May 31, 2012. All materials contained herein reflect the views of the faculty, and not those of IMER, the CME provider, or the commercial supporter. These materials may discuss therapeutic products that have not been approved by the US Food and Drug Administration and off-label uses of approved products. Readers should not rely on this information as a substitute for professional medical advice, diagnosis, or treatment. The use of any information provided is solely at the user’s own risk, and all users should verify the prescribing information and all data before treating patients or employing any therapeutic products described in this educational activity.
A lysosomal storage disease caused by acid sphingomyelinase deficiency (ASMD), which catalyzes the hydrolysis of sphingomyelin (SM) to ceramide and phosphocholine.
detailed discussion on cytogenetics in CML - Pathophysiology, treatment, TKI Resistance, Mutation analysis timing, various mutations in CML, BCR-ABL1 Variants, Significance of mutations and management.
This presentation contains all the updated information regarding ongoing treatment protocol, HSCT, Antibiotic prophylaxis, upcoming targeted therapies related to AML
Disease of older males.
The Philadelphia chromosome - BCR-ABL gene and it’s Tyrosine kinase protein – central to the pathogenesis.
Occurs in 3 phases
Imatinib has revolutionized the management
Roundtable Expert Discussions on CML Clinical Debates: A Collaborative Video Viewpoint Series With Medscape
This video viewpoint in its original and unaltered format is for educational purposes and is current as of May 31, 2012. All materials contained herein reflect the views of the faculty, and not those of IMER, the CME provider, or the commercial supporter. These materials may discuss therapeutic products that have not been approved by the US Food and Drug Administration and off-label uses of approved products. Readers should not rely on this information as a substitute for professional medical advice, diagnosis, or treatment. The use of any information provided is solely at the user’s own risk, and all users should verify the prescribing information and all data before treating patients or employing any therapeutic products described in this educational activity.
A lysosomal storage disease caused by acid sphingomyelinase deficiency (ASMD), which catalyzes the hydrolysis of sphingomyelin (SM) to ceramide and phosphocholine.
detailed discussion on cytogenetics in CML - Pathophysiology, treatment, TKI Resistance, Mutation analysis timing, various mutations in CML, BCR-ABL1 Variants, Significance of mutations and management.
This presentation contains all the updated information regarding ongoing treatment protocol, HSCT, Antibiotic prophylaxis, upcoming targeted therapies related to AML
Conferència a càrrec d'Enriqueta Felip. Oncòloga de l'Hospital Vall d'Hebron de Barcelona. Líder en la investigació de càncer de pulmó al VHIO. Membre de la Junta Directiva de la Societat Espanyola d'Oncologia Mèdica i de l'European Society for Medical Oncology. En el marc de la Jornada "Els nous reptes de la Medicina de Precisió" organitzada el 12 de novembre per la Societat Catalana de Gestió Sanitària
Newer biomarkers,techniques & their inclusion in 2016 WHO classification for leukaemia/lymphomas increases the responsibility of the pathologists, requiring to develop an integrated multidisciplinary approach for reporting.
Clinical diagnosis of chronic myeloid leukemia by real time polymerase chain ...Teboho Mooko
Oncology study i did in my third year (2014). the study was basically about monitoring Chronic Myeloid leukemia (CML) using Real-Time PCR techniques to check how patients from Universitas Hospital responded to the treatment of Gleevec drug.
diffuse large B cell lymphoma recent molecular classification
molecular classification and their time frame with references
Recent advantages of DLBCL and thier implication in therapy
Lecture By:
Dr Hisham Fakher
Consultant Hematology
Medical Director of Regional Laboratory and Central Blood Bank
Ministry of Health –Almadinah Almonawarah
MPN MLPA screening analysis is a comprehensive testing to identify panel gene hotspot mutations (JAK2, MPL & CALR) associated with the myeloproliferative neoplasms including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). Mutation analysis helps to differentiate reactive conditions from MPNs, may provide prognostic information, and may help distinguish ET and PMF from PV. DDRC SRL Diagnostic network in Kerala offers MPN MLPA Screening Panel test for the customers.
COVID-19 affects each of the people in various ways. Most infected people will develop mild to severe illness and recover without hospitalization. Seek immediate medical services if you have severe symptoms. Typical symptoms of COVID-19.
* Shortness of breath
* Loss of movement
* Chest pain
* Fever
* Cough
* Sore throat
* Headache
Breast cancer is known as the cancer that grow up in the cells of breasts. Breast cancer is the most typical cancer detected in the women. We are celebrating October month as the breast cancer awareness month. It helps the women to get more information about the breast cancer. DDRC SRL diagnostics center in Kerala provides free mammography campaigns for the women in Kerala in this breast cancer awareness month.
Coronavirus disease (COVID - 19) is a communicable respiratory disease caused by a new virus, COVID - 19. Symptoms range mild to moderate respiratory illness and take 2-14 days after exposure to develop. Those with a weakened immune system and older people with underlying medical problems like cardiovascular diseases, respiratory diseases, diabetes and cancer are more likely to develop a critical illness. There is no current vaccine or treatment for COVID - 19 disease.
Knowing the appropriate ways to wear, take off, clean, and store face masks are critical. Using face masks incorrectly may hamper its effectiveness in reducing the risk of virus transmission. Get to know about the face mask management.
Cytogenetics test involves the analysis of chromosomes for the genetic disorders. This test includes culture of blood Lymphocytes/bone marrow cells, followed by banding and analysis of meta-phase cells.
Buying food and keeping safe during COVID-19. We should more careful while we are in shopping. Keep yourself & products clean and safe as we don't know about the COVID-19 infection.
Know about how to use a mask perfectly in this CORONA season. Before wearing a mask, you need to wash hands with soap and water. Don't re-use the mask, it will cause infection.
Symptoms Of Fatty Liver (Hepatic Steatosis)DDRCSRL
Fatty Liver is also known as Hepatic Steatosis. It is caused by the excess build up of fat in the liver. Know about the common symptoms of Fatty Liver.
Jaundice is a symptom of underlying diseases in our body mainly in the liver. Do you know about the cause, diagnosis methods, preventive measures of jaundice? Know about it through this presentation.
Navigating Challenges: Mental Health, Legislation, and the Prison System in B...Guillermo Rivera
This conference will delve into the intricate intersections between mental health, legal frameworks, and the prison system in Bolivia. It aims to provide a comprehensive overview of the current challenges faced by mental health professionals working within the legislative and correctional landscapes. Topics of discussion will include the prevalence and impact of mental health issues among the incarcerated population, the effectiveness of existing mental health policies and legislation, and potential reforms to enhance the mental health support system within prisons.
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
CRISPR-Cas9, a revolutionary gene-editing tool, holds immense potential to reshape medicine, agriculture, and our understanding of life. But like any powerful tool, it comes with ethical considerations.
Unveiling CRISPR: This naturally occurring bacterial defense system (crRNA & Cas9 protein) fights viruses. Scientists repurposed it for precise gene editing (correction, deletion, insertion) by targeting specific DNA sequences.
The Promise: CRISPR offers exciting possibilities:
Gene Therapy: Correcting genetic diseases like cystic fibrosis.
Agriculture: Engineering crops resistant to pests and harsh environments.
Research: Studying gene function to unlock new knowledge.
The Peril: Ethical concerns demand attention:
Off-target Effects: Unintended DNA edits can have unforeseen consequences.
Eugenics: Misusing CRISPR for designer babies raises social and ethical questions.
Equity: High costs could limit access to this potentially life-saving technology.
The Path Forward: Responsible development is crucial:
International Collaboration: Clear guidelines are needed for research and human trials.
Public Education: Open discussions ensure informed decisions about CRISPR.
Prioritize Safety and Ethics: Safety and ethical principles must be paramount.
CRISPR offers a powerful tool for a better future, but responsible development and addressing ethical concerns are essential. By prioritizing safety, fostering open dialogue, and ensuring equitable access, we can harness CRISPR's power for the benefit of all. (2998 characters)
The Importance of Community Nursing Care.pdfAD Healthcare
NDIS and Community 24/7 Nursing Care is a specific type of support that may be provided under the NDIS for individuals with complex medical needs who require ongoing nursing care in a community setting, such as their home or a supported accommodation facility.
One of the most developed cities of India, the city of Chennai is the capital of Tamilnadu and many people from different parts of India come here to earn their bread and butter. Being a metropolitan, the city is filled with towering building and beaches but the sad part as with almost every Indian city
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
CHAPTER 1 SEMESTER V PREVENTIVE-PEDIATRICS.pdfSachin Sharma
This content provides an overview of preventive pediatrics. It defines preventive pediatrics as preventing disease and promoting children's physical, mental, and social well-being to achieve positive health. It discusses antenatal, postnatal, and social preventive pediatrics. It also covers various child health programs like immunization, breastfeeding, ICDS, and the roles of organizations like WHO, UNICEF, and nurses in preventive pediatrics.
Empowering ACOs: Leveraging Quality Management Tools for MIPS and BeyondHealth Catalyst
Join us as we delve into the crucial realm of quality reporting for MSSP (Medicare Shared Savings Program) Accountable Care Organizations (ACOs).
In this session, we will explore how a robust quality management solution can empower your organization to meet regulatory requirements and improve processes for MIPS reporting and internal quality programs. Learn how our MeasureAble application enables compliance and fosters continuous improvement.
Defecation
Normal defecation begins with movement in the left colon, moving stool toward the anus. When stool reaches the rectum, the distention causes relaxation of the internal sphincter and an awareness of the need to defecate. At the time of defecation, the external sphincter relaxes, and abdominal muscles contract, increasing intrarectal pressure and forcing the stool out
The Valsalva maneuver exerts pressure to expel faeces through a voluntary contraction of the abdominal muscles while maintaining forced expiration against a closed airway. Patients with cardiovascular disease, glaucoma, increased intracranial pressure, or a new surgical wound are at greater risk for cardiac dysrhythmias and elevated blood pressure with the Valsalva maneuver and need to avoid straining to pass the stool.
Normal defecation is painless, resulting in passage of soft, formed stool
CONSTIPATION
Constipation is a symptom, not a disease. Improper diet, reduced fluid intake, lack of exercise, and certain medications can cause constipation. For example, patients receiving opiates for pain after surgery often require a stool softener or laxative to prevent constipation. The signs of constipation include infrequent bowel movements (less than every 3 days), difficulty passing stools, excessive straining, inability to defecate at will, and hard feaces
IMPACTION
Fecal impaction results from unrelieved constipation. It is a collection of hardened feces wedged in the rectum that a person cannot expel. In cases of severe impaction the mass extends up into the sigmoid colon.
DIARRHEA
Diarrhea is an increase in the number of stools and the passage of liquid, unformed feces. It is associated with disorders affecting digestion, absorption, and secretion in the GI tract. Intestinal contents pass through the small and large intestine too quickly to allow for the usual absorption of fluid and nutrients. Irritation within the colon results in increased mucus secretion. As a result, feces become watery, and the patient is unable to control the urge to defecate. Normally an anal bag is safe and effective in long-term treatment of patients with fecal incontinence at home, in hospice, or in the hospital. Fecal incontinence is expensive and a potentially dangerous condition in terms of contamination and risk of skin ulceration
HEMORRHOIDS
Hemorrhoids are dilated, engorged veins in the lining of the rectum. They are either external or internal.
FLATULENCE
As gas accumulates in the lumen of the intestines, the bowel wall stretches and distends (flatulence). It is a common cause of abdominal fullness, pain, and cramping. Normally intestinal gas escapes through the mouth (belching) or the anus (passing of flatus)
FECAL INCONTINENCE
Fecal incontinence is the inability to control passage of feces and gas from the anus. Incontinence harms a patient’s body image
PREPARATION AND GIVING OF LAXATIVESACCORDING TO POTTER AND PERRY,
An enema is the instillation of a solution into the rectum and sig
How many patients does case series should have In comparison to case reports.pdfpubrica101
Pubrica’s team of researchers and writers create scientific and medical research articles, which may be important resources for authors and practitioners. Pubrica medical writers assist you in creating and revising the introduction by alerting the reader to gaps in the chosen study subject. Our professionals understand the order in which the hypothesis topic is followed by the broad subject, the issue, and the backdrop.
https://pubrica.com/academy/case-study-or-series/how-many-patients-does-case-series-should-have-in-comparison-to-case-reports/
How many patients does case series should have In comparison to case reports.pdf
Acute Lymphoblastic Leukemia
1. Cytogenetics
To diagnose and monitor the treatment response of patients with B-cell
acute lymphoblastic leukemia (B-ALL).
Fluorescence In Situ Hybridization (FISH).
This test includes culture of blood / bone marrow cells, followed by
analysis of special sites of the chromosome using fluorescent probes.
BCR-ABL1, t(9:22)(q34;q11).
4 working days.
Bone marrow (Preferably)/ Peripheral blood.
3-4 ml bone marrow / 4 ml blood in green top heparin tube.
Label the vacutainer with patient’s name and specimen type.
The specimen should be shipped at 18-25°C, do not freeze.
It should reach the lab within 24 hours of collection
(preferably at the earliest). In case of delay, sample
to be stored on the door shelf of the refrigerator.
Complete blood count and bone marrow/ peripheral blood blast percentage must be provided.
Specify detailed clinical history, collection date & time of sample withdrawn on test request form*.
BCR-ABL1, t(9:22)(q34;q11)
¤ Present in 25% adult B-ALL
and 2-4% Pediatric B-ALL cases.
¤ Results in chimeric fusion protein
with constitutive tyrosine kinase activity.
¤ Presence of BCR-ABL1 fusion
gene confers a poor prognosis.
Test Purpose :
Methodology :
Testing Procedure :
Gene Analyzed :
Turn Around Time :
Specimen Type :
Specimen Volume :
Collection
instructions :
Specimen Transport :
Mandatory
Requirements :
Significance of :
Gene tested
Molecular Cytogenetic Analysis for
Acute Lymphoblastic Leukemia
2. Corporate Office & Referral Laboratory,
DDRC SRL Towers, Panampilly Nagar, Kochi-682 036
Ph: +91 484 2318222, 2318223
email: info@ddrcsrl.com
www.ddrconline.com, www.ddrclab.com
94977 17850
94977 17842
Highlights of the Test:
¤ Identification of specific BCR-ABL1 gene rearrangement
is critical for disease evaluation, optimal risk stratification and
treatment planning.
¤ BCR-ABL1 fusion gene amplification status, one of the prime
factors for drug resistance and adverse prognosis in ALL, is
also evaluated by this assay.
¤ This test is able to identify masked and variant Ph
(Philadelphia Chromosome) translocations which are not
detected by conventional cytogenetics and PCR primers.
¤ A single FISH assay can cover all the splice variants of
BCR-ABL1 fusion gene including b2a2 or b3a2 coding for
p210; e1a2 coding for p190; e19a2 (c3a2) coding for p230.
¤ Sensitivity and Accuracy: For initial diagnosis, 100-200
interphase nuclei are counted. For therapeutic response
analysis, with a previously abnormal result, up to 500
interphase nuclei may be counted.
¤ CE-IVD approved LSI BCR/ABL1 dual color, dual fusion
translocation probe kit is used for the assay.
Note:
A repeat sample may be needed in case
of culture failure.
If further testing is required, additional
charges may be incurred.
*Samples shall be rejected if above
details are not provided.