The document discusses the cellular events involved in inflammation, particularly leukocyte recruitment, activation, and the processes of margination, rolling, adhesion, and transmigration of leukocytes. It outlines various mechanisms by which leukocytes respond to injury and migrate towards chemotactic stimuli, including phagocytosis and the formation of neutrophil extracellular traps (NETs). Additionally, it covers the potential outcomes of acute inflammation, including resolution, scarring, and the development of chronic inflammation.

1. Inflammation
Fahad Asim
Pharm.D. (PU), R.Ph. (Pb.),
M.Phil. (Pharmacology)

2. Cellular Events: Leukocyte
Recruitment and activation
Leukocyte Recruitment
 Margination and rolling along the vessel
 Firm adhesion to the endothelium
 Transmigration between endothelial cells
 Migration in interstitial tissues toward a chemotactic stimulus

3. Margination and Rolling
 With increased vascular permeability, fluid leaves the vessel
causing leukocytes to settle-out of the central flow column and
“marginate” along the endothelial surface
 Endothelial cells and leukocytes have complementary surface
adhesion molecules which briefly stick and release causing
the leukocyte to roll along the endothelium like a tumbleweed
until it eventually comes to a stop as mutual adhesion reaches
a peak

4. Margination and Rolling
 Early rolling adhesion mediated by selectin family:
 E-selectin (endothelium)
 P-selectin (platelets, endothelium)
 L-selectin (leukocytes)
 Bind surface molecules (i.e.,CD34, Sialyl-Lewis X-
modified GP) that are up regulated on endothelium by
cytokines (TNF, IL-1) at injury sites

5.  Emigration of leukocytes: margination, rolling, adhesion,
diapedesis, migration
Neutrophils predominate (6-24 hours) and replaced by
Monocytes (24-48 hours)
Upregulation of molecules
 E-selectin (endothelium) - rolling, adhesion [induced by IL-1
and TNF]
P-selectin (endothelium, platelets) - rolling [present in Weibel-
Palade bodies]
L-selectin (leukocytes) - homing

6. Adhesion
 Rolling comes to a stop and adhesion results
 Other sets of adhesion molecules participate:
 Endothelial: ICAM-1, VCAM-1
 Leukocyte: LFA-1, Mac-1, VLA-4
(ICAM-1 binds LFA-1/Mac-1, VCAM-1 binds VLA-4)
 Ordinarily down-regulated or in an inactive conformation, but
inflammation alters this by chemokines along with cytokines
e.g. IL-1, TNF

7.  ICAM (interacts with integrins LFA-1 on leukocytes)
adhesion, arrest, transmigration
 VCAM (interacts with integrins on eos, monos, lymphs)
adhesion
 PECAM-1 (CD31) platelet-endothelial cell adhesion molecule
 Upon stimulation by chemokines integrins undergo
conformational changes and cluster together with high affinity

8. Emigration and chemotaxis
 Emigration is a mechanism by which the leukocytes extend
pseudopodia, pass through the capillary walls by ameboid
movement, and migrate into the tissue spaces.
 The emigration of leukocytes also may be accompanied by an
escape of red blood cells.
 Once they have exited the capillary, the leukocytes move
through the tissue guided by secreted cytokines, bacterial and
cellular debris, and complement fragments (C3a, C5a).
 The process by which leukocytes migrate in response to a
chemical signal is called chemotaxis.

9. Transmigration
 Leukocytes migrate through vessel wall by squeezing
between cells at intercellular junctions (diapedesis)
 Occurs mainly within the systemic venules and
pulmonary capillaries via PECAM –1 (platelet endothelial
adhesion molecule 1) (CD31).
It is expressed on both leukocytes and endothelial cells.
 Must then cross basement membrane by collagenases
 Early in inflammatory response mostly PMNs, but as
cytokine and chemotactic signals change with
progression of inflammatory response, alteration of
endothelial cell adhesion molecule expression activates
other populations of leukocytes to adhere (monocytes,
lymphocytes, etc)

11. Chemotaxis
 Leukocytes follow chemical gradient to site of injury (chemotaxis)
 Soluble bacterial products (N-formyl methionine peptides)
 Complement components (C5a)
 Cytokines (chemokine family e.g., IL-8)
 LTB4 (AA metabolite)
 Chemotactic agents bind surface receptors (G protein-coupled
receptors)  signal transduction influx of calcium  assembly
of cytoskeletal contractile elements.
 Leukocytes extend pseudopods with overlying surface adhesion
molecules (integrins) that bind ECM during chemotaxis which
pulls the leukocyte in direction of attachment

12. Chemotaxis
 In acute inflammation neutrophils predominate in the
inflammatory infiltrate during the first 6 to 24 hours and are
replaced by monocytes in 24 to 48 hours.
 Most numerous leukocytes
 Attach more firmly to the adhesion molecules
 Neutrophils are short live-they die by apotosis and disappear within 24 to
48 hours.
 Monocytes survive longer

14. Leukocyte Activation
 Leukocyte activation results in the enhancement
 Phagocytosis of particles
 Intracellular destruction of phagocytosed
microbes and dead issues
 Liberation of substances that destroy
extracellular microbes and dead tissues
 Production of mediators

15. Phagocytosis
 During the next and final stage of the cellular response, the
neutrophils and macrophages engulf and degrade the
bacteria and cellular debris in a process called phagocytosis.
 Phagocytosis involves three distinct steps:
 Recognition and attachment of the particle to the ingesting
leukocyte
 Engulfment, with subsequent formation of a phagocytic
vacuole
 Killing and degradation of the ingested material
 If the antigen is coated with antibody or complement, its
adherence is increased because of binding to complement.
This process of enhanced binding of an antigen caused by
antibody or complement is called opsonization.

16. Phagocytosis
 Recognition and attachment via opsonization
(Fc fragment of IgG, C3b, collectins)
·
 Engulfment via binding of the opsonized
particle to the FcγR
 Corresponding receptors on leukocytes (FcR,
CR1, 2, C1q) leads to binding

17. Killing and Degradation of
Phagocytosed Microbes
 Triggers an oxidative burst ,engulfment and formation of
vacuole which fuses with lysosomal granule membrane
(phagolysosome)
 Granules discharge within phagolysosome and
extracellularly (degranulation)
 The most imp. Microbiocidal substances are ROS and
lysosomal enzymes.

18. Oxidative burst
 Phagocytosis stimulates an Oxidative burst
charecterized by
 Increase in oxygen consumption
 Glycogenolysis
 Increase glucose oxidation
 Production of ROS
 Formation of superoxide ion
 2O2 + NADPH  2O2
-rad
+ NADP+
+ H+
(NADPH oxidase)
 O2 + 2H+
 H2O2 (dismutase)

19. Reactive oxygen species
 Hydrogen peroxide alone insufficient
 MPO (azurophilic granules) converts
hydrogen peroxide to HOCl-
(in presence of
Cl-
), an oxidant/antimicrobial agent
 Therefore, PMNs can kill by halogenation, or
lipid/protein peroxidation

20. Degradation and Clean-up
 Reactive end-products only active within
phagolysosome
 Hydrogen peroxide broken down to water
and oxygen by catalase
 Dead microorganisms degraded by
lysosomal acid hydrolases

21. Leukocyte granules
 Other antimicrobials in leukocyte granules:
 Bactericidal permeability increasing protein (BPI)
 Lysozyme
 Lactoferrin
 Defensins (punch holes in membranes)

22. Secretion of Microbicidal
Substances
 Leukocytes also secrete elastase which destroy and digest
extracellular microbes and dead tissues, as well as
antimicrobial peptides.
 Phagocytic vacuole may remain transiently open to the
outside before complete closure of the phagolysosome
(regurgitation during feeding).
 If cells encounter materials that cannot be easily ingested,
such as immune complexes deposited on immovable
surfaces (e.g. glomerular basement membrane). Attempt to
phagocytose these substances (frustrated phagocytosis)
triggers strong leukocyte activation
 The membrance of the phagolysosome may be damaged if
potentially injurious substances.

23. Neutrophil Extracellular Traps
(NETs)
 Neutrophils: These traps are extracellular fibrillar networks that
are produced by neutrophils in response to infectious
pathogens, inflammatory mediators.
 Contain a framework of nuclear chormatin with embedded
granule proteins, such as antimicrobial peptides and enzymes
 The traps provide a high concentration of the antimicrobial
substances at sites of infection, and prevent the spread of the
microbes by trapping them in the fibrills. In the process, the
nuclei of the neutrophils are lost, leading to death of the cells.
NETs also have been detected in blood neutrophils during
sepsis.

25. Leukocyte-Induced Tissue Injury
 Because leukocytes secrete harmful substances e.g. enzymes ,
ROS, they are important cause of injury to normal cells and
tissues under several circumstances
 “Bystander tissues” are injured.
 In tuberculosis and some viral diseases, contributes more to
the pathologic process than does the microbe itself.
 As a normal attempt to clear damaged and dead tissues (e.g.
myocardial infarction). In an infarct, inflammation may prolong
and exacerbate the injurious consequences of the ischemia.
 When the inflammatory response is inappropriately directed
against host tissues, as in certain autoimmune diseases, or when
the host reacts excessively against nontoxic environmental
substances, such as asthma
 Activated leukocytes, especially macrophages, also secrete may
cytokines, which stimulate further inflammation and have
important systemic effects

26. Defects in leukocyte function
 Defects of adhesion:
 In LAD-1: LFA-1 and Mac-1 subunit defects lead to
impaired adhesion
 In LAD-2: Absence of sialyl-Lewis X, and defect in E-
and P-selectin sugar epitopes
 Defects in phagolysosome formation
 Microtubule assembly defect leads to impaired
locomotion and lysosomal degranulation (Chediak-
Higashi Syndrome)
 Defects of microbicidal activity:
 Deficiency of phagocyte oxidase that generates
ROS, therefore no oxygen-dependent killing
mechanism (chronic granulomatous disease)

27. OUTCOMES OF ACUTE INFLAMMATION
 1) RESOLUTION
 2) SCARRING
 3) CHRONIC INFLAMMATION

28. Resolution
 The complete restoration of the inflamed tissue
back to A normal status. Inflammatory measures
such as vasodilation, chemical production, and
leukocyte infiltration cease, and damaged
parenchymal cells regenerate. In situations
where limited or short lived inflammation has
occurred this is usually the outcome.

29. Fibrosis
 Large amounts of tissue destruction, or
damage in tissues unable to regenerate, can
not be regenerated completely by the body.
 Fibrous scarring occurs in these areas of
damage, forming a scar composed primarily of
collagen.
 The scar will not contain any specialized
structures, such as parenchymal cells, hence
functional impairment may occur.

30. Chronic inflammation
 In acute inflammation, if the injurious agent persists
then chronic inflammation will ensue. This process,
marked by inflammation lasting many days, months or
even years, may lead to the formation of a chronic
wound.
 Chronic inflammation is characterized by the
dominating presence of macrophages in the injured
tissue.
 These cells are powerful defensive agents but the
toxins they release are injurious to the organism's own
tissues Consequently, chronic inflammation is almost
always accompanied by tissue destruction.

31.  Outcomes of acute inflammation: resolution, healing by scarring (fibrosis), or chronic inflammation.

32. Morphologic Patterns of Acute
Inflammation
 Serous inflammation
 Charterised by Watery relatively protein-poor fluid
 Derived from the plasma or secretions of
mesothelial cells lining the peritoneal, pleural, and
pericardial cavities.
 Skin blister (burn or viral infection)
 Fluid in a serous cavity is called an effusion

33. BLISTER, “Watery”, i.e., SEROUS

34. Serous inflammation. Low-power view of a cross-section
of a skin blister showing the epidermis separated from the dermis by a
focal collection of serous effusion.

35. Serous
Inflammation
- Effusion

36. Morphologic Patterns of Acute
Inflammation
 Fibrinous inflammation
 Occurs as a consequence of severe injuries,
resulting in greater vascular permeability that allows
large molecules such as fibrinogen to pass the
endothelial barrier.
 A fibrinous exudate is characteristic of inflammation
in the lining of body cavities, such as the meninges
pericardium, and pleura.
 Such exudate is degraded by fibrinolysis, and the
accumulated debris may be removed by
macrophages resulting in restoration of the normal
tissue structure (restoration)
 Organization

37. Fibrinous
Inflammation

38. Fibrinous pericarditis. A, Deposits of fibrin on the pericardium. B, A
pink meshwork of fibrin exudate (F) overlies the pericardial
surface (P).

39. Morphologic Patterns of Acute
Inflammation
 Suppurative (purulent) inflammation and abscess
formation
 Collection of purulent exudate (pus) consisting of
neutrophils, necrotic cells, and edema fluid. Certain
organisms e.g. staphylococci are more likely to induce
such localized suppuration and are therefore pyogenic
pus forming.
 Abscesses: Focal collection of pus caused by pyogenic
organisms into a tissue or by secondary infections of
necrotic foci.
 Abscesses typically have a central, largely necrotic region
rimmed by a layer rimmed by a layer of neutrophils, dilated
vessels and fibroblast prolifation
 As time passes, the abscess may become completely
walled off and eventually be replaced by connective
tissue.
 Because of the underlying tissue destruction, the usual
outcome with abscess formation is scarring.

40. Purulent -
Inflammation -
PUS

41. Purulent - Inflammation - PUS
 Purulent inflammation with abscess formation. A, Multiple bacterial abscesses in the lung
(arrows) in a case of bronchopneumonia.
 B, The abscess contains neutrophils and cellular debris and is surrounded by congested blood
vessels.

42. PUS
=
PURULENT
ABSCESS
=
POCKET
OF
PUS
=
NEUTROPHILS

43. Morphologic Patterns of Acute
Inflammation
 Ulcer is a local defect, or excavation, of the
surface of an organ or tissue that is produced by
necrosis of cells and sloughing (shedding) of
necrotic and inflammatory tissue.
 Inflammation exist on or near a surface. Ulcer are
most commonly encountered in the mucosa of a
mouth, stomach, intestines, or genitourinary tract
and (2) in the subcutaneous tissues of lower
extremities in older persons who have circulation
disturbances predisposing affected tissue to
extensive necrosis. Ulcerations are best
exemplified by peptic ulcer of the stomach or
duodenum, in which acute and chronic
inflammation coexist.

44.  Ulcer. A, A chronic duodenal ulcer. B, Low-power cross-section
of a duodenal ulcer crater with an acute inflammatory exudate in
the base.