This document summarizes the enzyme-linked immunosorbent assay (ELISA) technique. ELISA uses antibodies and antigens to detect the presence and quantity of substances like proteins, peptides, hormones, and antibodies. There are four main types of ELISA - direct, indirect, sandwich, and competitive - which differ based on whether antigens or antibodies are fixed to the plate and whether primary or secondary antibodies are used. ELISA has various applications in medicine, food testing, and toxicology for detecting specific antigens or antibodies in samples.