Central Line-Associated Bloodstream Infections (CLABSI) are preventable healthcare-associated infections often due to negligence, such as poor hand hygiene and improper catheter management. Effective prevention strategies include proper aseptic techniques, education for healthcare workers, and following CDC guidelines. Research indicates improved hand hygiene and effective training significantly reduce the incidence of CLABSI, showcasing the need for stringent adherence to preventive measures in healthcare settings.