1st JAIMA-VINALab Joint Seminar Dec. 16th, 2021 Microplastics AnalysisJASCO EUROPE s.r.l. JASCO INCORPORATED JASCO INTERNATIONAL CO., LTD. JASCO CHINA (SHANGHAI) CO., LTD. JASCO COPRPORATION Global business of JASCOSize ~100 μｍ (Macro) 100~5 μm (Micro) 5~1 μｍ (Micro) Target Ocean River Oceans Rivers Atmosphere Organisms* Atmosphere Organisms* Analysis details • Identification • Shape observation • Number of pieces per unit area MPs' needs *MPs in organisms are often less than 1 µm in size, making them difficult to measure.Size ~100 μｍ (Macro) 100~5 μm (Micro) 5~1 μｍ (Micro) JASCO's proposal Hardware • Macro FTIR Software • Advanced Spectrum Search (ADSS) • KnowItAll Hardware • Sampling Kits • Microscopic FTIR • Microscopic Raman Software • ADSS • KnowItAll • Jasco Particle Analysis Hardware • Sampling Kits • Microscopic Raman Software • ADSS • KnowItAll • Jasco Particle Analysis JASCO’s solution for sample of different sizeSampling, Observation, Measurement ◆ FT/IR-4X + ATR PRO 4X VIEW Unidentified MPs 1.1 mm 0.8 mm Macro Observed image ◆ Advanced spectrum search, KnowItAll Sample source ： e-kagaku the Association of the Global Science Education -Biwako Environment Project 2021- https://global-science.or.jp/eng/ https://e-kagaku.com/ Unidentified MPs Polyester Wavenumber [cm-1] Wavenumber [cm-1] Classification : Polyester Wavenumber [cm-1] Measurement spectrum Wavenumber [cm-1] Spectrum classify PolyesterApplication ◆ FT/IR-4X + ATR PRO 4X VIEW Unidentified MPs Macro 1 mm 0.2 mm 1 mm 0.2 mm Measurement spectrum Measurement spectrum Database : Polyvinyl chloride, PVC Database : Polypropylene resin Wavenumber [cm-1] Wavenumber [cm-1] Wavenumber [cm-1] Wavenumber [cm-1] Abs Abs Abs AbsSampling ◆ Sampling Kits • Suction filtration kits for φ25 mm membrane filters • Membrane filter lineup with different materials and pore size No. Material Pore size 1 PTFE 10 μm 2 PTFE 5 μm 3 PTFE 1 μm 4 Alumina 0.2 μm Membrane filter lineup Suction filtration kit Membrane filters MicroObservation, Measurement ◆ IR: FT/IR-4X + IRT-5200, 7200 Micro Observation (about 100～5 µm sample)The following measurement methods are selectable for both microscopic IR/Raman Observation, Measurement Point measurement Multipoint measurement Imaging (Mapping) Measurement MicroObservation, Measurement, Qualitative Advanced Search NAV (Multipoint) Real-time display Automatic registration Display of results Search NAV (Multipoint) S1 S3 S2 S4 S5 S6 TiO2 PMMA TiO2 PET PS PS ●PS ●PET ●PMMA ●TiO2 ◆ IR: IRT-5200, 7200 ◆ Raman: NRS-4500 ◆ ADSS MicroParticle size analysis (Imaging Measurement) ■ PS ■ PE ■ PP ■ PVC ■ PET Measurement Results (Color map) Stacked Histogram of each component Data source： TOSOH Analysis and Research Center Co., Ltd. size [µm] counts total Micro ◆ JASCO Particle AnalysisObservation, Measurement ◆ IR: FT/IR-4X + IRT-5200, 7200 Micro ◆ Accessory: IQ Frame ◆ Raman: NRS-4500FTIR: material composition (PE) Raman: pigments of PE Observation, Measurement,

1. 1st JAIMA-VINALab Joint Seminar
Dec. 16th, 2021
Microplastics Analysis

2. JASCO INCORPORATED
JASCO EUROPE s.r.l.
JASCO INTERNATIONAL CO., LTD.
JASCO CHINA (SHANGHAI) CO., LTD.
JASCO COPRPORATION
Global business of JASCO

3. Size
~100 μｍ
(Macro)
100~5 μm
(Micro)
5~1 μｍ
(Micro)
Target
Ocean
River
Oceans
Rivers
Atmosphere
Organisms*
Atmosphere
Organisms*
Analysis
details
• Identification
• Shape observation
• Number of pieces per unit area
MPs' needs
*MPs in organisms are often less than 1 µm in size, making them difficult to measure.

4. Size
~100 μｍ
(Macro)
100~5 μm
(Micro)
5~1 μｍ
(Micro)
JASCO's
proposal
Hardware
• Macro FTIR
Software
• Advanced
Spectrum
Search (ADSS)
• KnowItAll
Hardware
• Sampling Kits
• Microscopic FTIR
• Microscopic Raman
Software
• ADSS
• KnowItAll
• Jasco Particle Analysis
Hardware
• Sampling Kits
• Microscopic Raman
Software
• ADSS
• KnowItAll
• Jasco Particle Analysis
JASCO’s solution for sample of different size

5. Sampling, Observation, Measurement
◆ FT/IR-4X + ATR PRO 4X VIEW Unidentified MPs
1.1 mm
0.8
mm
Macro
Observed image
◆ Advanced spectrum search, KnowItAll Sample source ：
e-kagaku
the Association of the Global Science Education
-Biwako Environment Project 2021-
https://global-science.or.jp/eng/
https://e-kagaku.com/
Unidentified MPs
Polyester
Wavenumber [cm-1]
Wavenumber [cm-1]
Classification : Polyester
Wavenumber [cm-1]
Measurement spectrum
Wavenumber [cm-1]
Spectrum classify
Polyester

6. Application
◆ FT/IR-4X + ATR PRO 4X VIEW
Unidentified MPs
Macro
1 mm
0.2 mm
1 mm
0.2 mm
Measurement
spectrum
Measurement
spectrum
Database : Polyvinyl chloride, PVC
Database : Polypropylene resin
Wavenumber [cm-1]
Wavenumber [cm-1]
Wavenumber [cm-1]
Wavenumber [cm-1]
Abs
Abs
Abs
Abs

7. Sampling
◆ Sampling Kits
• Suction filtration kits for φ25 mm membrane filters
• Membrane filter lineup with different materials and pore size
No. Material Pore size
1 PTFE 10 μm
2 PTFE 5 μm
3 PTFE 1 μm
4 Alumina 0.2 μm
Membrane filter lineup Suction filtration kit
Membrane filters
Micro

8. Observation, Measurement
◆ IR: FT/IR-4X + IRT-5200, 7200
Micro
Observation
(about 100～5 µm sample)

9. The following measurement methods are selectable for both
microscopic IR/Raman
Observation, Measurement
Point
measurement
Multipoint
measurement
Imaging
(Mapping)
Measurement
Micro

10. Observation, Measurement, Qualitative
Advanced Search NAV
(Multipoint)
Automatic registration
Real-time display Display of results
Search NAV
(Multipoint)
S1
S2
S3
S4
S5
S6
TiO2
TiO2
PMMA
PET
PS
PS ●PS
●PET
●PMMA
●TiO2
◆ IR: IRT-5200, 7200
◆ Raman: NRS-4500
◆ ADSS
Micro

11. Particle size analysis (Imaging Measurement)
■ PS ■ PE
■ PP ■ PVC
■ PET
Measurement
Results (Color map)
Stacked Histogram of each component
Data source：
TOSOH Analysis and Research Center Co., Ltd.
size [µm]
counts
total
Micro
◆ JASCO Particle Analysis

12. Observation, Measurement
◆ IR: FT/IR-4X + IRT-5200, 7200
Micro
◆ Accessory: IQ Frame
◆ Raman: NRS-4500

13. FTIR: material composition (PE)
Raman: pigments of PE
Observation, Measurement, Qualitative
Pigment (blue)
Spectra
Measurement Results
(Color map)
Observed image
4000 1000
2000
3000
Abs
Wavenumber [cm-1]
3800 100
1000
2000
3000
Int.
Raman Shift [cm-1]
IR
Raman
PE
Pigment (yellow)
teflon
Micro
◆ ADSS, KnowItAll

14. Analysis of food packaging films
Micro
◆ IQ Frame, ADSS, KnowItAll
4000 1000
2000
3000
Wavenumber [cm-1]
Abs.
cellulose
PP
PE
PET
PP
PE
PET
TiO2
Int.
500
1000
2000
3000
Raman Shift [cm-1]
Observed image
Microscopic FTIR Microscopic Raman
Foreign matter

15. Summary
• Sample size is one indicator of instrument
selection.
• Tools for sampling, measurement, qualitative
analysis and particle size analysis are available.
• FTIR and Raman have a complementary
relationship.

16. Thank you very much
for your attention!
Mascot: JASCO-kun
Global site
JASCO’s HP
If you have any questions, please contact :
shingo.norimoto@jasco.co.jp
d1s1@jascoint.co.jp