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3. Let's suppose the A600 of your 1:100 diluted culture is 0.8 . If you want to inoculate 1108 cells /
ml on a plate, how much volume would you take from your original culture tube and how much LB
media would you take to get the final volume of 1ml in a new tube. (2.5 points) A. 2.5l of original
culture into 997.5l of LB media B. 250l of original culture into 750l of LB media C. 25l of original
culture into 975l of LB media D. 0.25l of original culture into 999.75l of LB media

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  • 1. 3. Let's suppose the A600 of your 1:100 diluted culture is 0.8 . If you want to inoculate 1108 cells / ml on a plate, how much volume would you take from your original culture tube and how much LB media would you take to get the final volume of 1ml in a new tube. (2.5 points) A. 2.5l of original culture into 997.5l of LB media B. 250l of original culture into 750l of LB media C. 25l of original culture into 975l of LB media D. 0.25l of original culture into 999.75l of LB media