This document outlines numerous safety rules for chemistry laboratories, including requiring appropriate eye protection, closed-toe shoes, and protective clothing. It specifies safe practices such as adding acids to water, heating test tubes at an angle away from people, and knowing how to use safety equipment and respond to emergencies. Students are instructed to clean up after themselves and dispose of waste properly before leaving the lab.
Microorganisms are all around us as Pasteur pointed out a century ago they play vital role in ecology of life on earth
In addition some microorganisms provide important commercial benefits through their use in production of chemical antibiotics and certain food .Microorganisms are also major tools in basic research in biology
Finally as we all know some microorganisms cause diseases in human animals and plants
lab coat or apron must be worn all times in labprotect you and your clothes when leave labremove coat apron
Lunches coats books that are not required for this lab should be stored some out of the way place desk space must be reserved for essential equipment and ab manual
Wear gloves for touching blood and body fluid nonintact skin or handle items soiled with body fluidwash your hands after remove glovesWear mask and protective eye wearin procedure that are likely to generate droplet of blood or other body fluid
Scrub down the top of your table
Make it habit to keep your hand away from your mouth eye nose
Use mechanical pipetting devicesdon not use mouth pipetting
When bacterial culture accidently spilled on the floor Notify the instructor to ensure proper disinfection procedure can be assuredfailure to report spill is sufficient reason to prevent you from returning to the lab
spilled bacterial microbial culture with paper toweldisinfect the toFor spills in or near the eye ,use the eyewashwels leave for 20 minutesthen clean the spill
Wash your hand after every laboratory exercisebecause bar soap may become contaminateDon not touch broken glasswaree with your hands use broom and dustpan place broken glasswaretransfer to be autoclaved materiald use liquid or powdered soaps
Microorganisms are all around us as Pasteur pointed out a century ago they play vital role in ecology of life on earth
In addition some microorganisms provide important commercial benefits through their use in production of chemical antibiotics and certain food .Microorganisms are also major tools in basic research in biology
Finally as we all know some microorganisms cause diseases in human animals and plants
lab coat or apron must be worn all times in labprotect you and your clothes when leave labremove coat apron
Lunches coats books that are not required for this lab should be stored some out of the way place desk space must be reserved for essential equipment and ab manual
Wear gloves for touching blood and body fluid nonintact skin or handle items soiled with body fluidwash your hands after remove glovesWear mask and protective eye wearin procedure that are likely to generate droplet of blood or other body fluid
Scrub down the top of your table
Make it habit to keep your hand away from your mouth eye nose
Use mechanical pipetting devicesdon not use mouth pipetting
When bacterial culture accidently spilled on the floor Notify the instructor to ensure proper disinfection procedure can be assuredfailure to report spill is sufficient reason to prevent you from returning to the lab
spilled bacterial microbial culture with paper toweldisinfect the toFor spills in or near the eye ,use the eyewashwels leave for 20 minutesthen clean the spill
Wash your hand after every laboratory exercisebecause bar soap may become contaminateDon not touch broken glasswaree with your hands use broom and dustpan place broken glasswaretransfer to be autoclaved materiald use liquid or powdered soaps
3. Wear appropriate protective
clothing
• Your clothing should cover your legs to the
knees – shorts are not appropriate for the
laboratory
• Lab aprons can be used to protect good
clothing
• Loose clothing should not be worn because it
may dip into chemicals or fall into a flame and
catch fire
4. Wear shoes that cover your
feet.
• Sandals and open-
toed shoes do not
protect your feet
from broken glass
that is frequently
found in the lab
• Also, leather shoes
protect your feet
from chemical spills
– canvas shoes do
not.
5. Do not apply cosmetics, eat, or
drink in the lab.
• These activities are ways by which you
can accidentally ingest harmful
chemicals
15. Do not smell any chemicals
directly!
• If absoluteley
necessary to
smell, use your
hand to fan the
vapor to your
nose.
16. Do not pipet solutions by mouth!
• Use a rubber
suction bulb or
other device to
fill a pipet.
17. Wash your hands with soap and
water before leaving.
• This rule applies
even if you have
been wearing
gloves!
18. Know the hazards of the
materials being used.
• Read and reread
labels carefully
to make sure that
you are using the
right chemical.
• Know how to
interpret data
from a MSDS.
19. Tie Back Loose Hair
• Dangling hair can
fall into the
Bunsen burner
and catch fire or
can fall into a
chemical solution
• P.S. Burning hair
REALLY STINKS!
20. Know the safety equipment
• Eye wash fountain
• Safety shower
• Fire extinguisher
• Emergency exits
21. Know how to use the safety
equipment
• Eye wash Fountain
• Safety Shower
• Fire extinguisher
• Emergency Exit
22. Know how to respond to an
emergency
Clarkson University 268-6400
Emergensy Number (after working hours) 268-6439
Campus Safety 268-6666
Fire Dep 9-265-3311
Hospital 9-265-3300/3304/5720
Police 9-265-2121/2122
Rescue Squad 911
(numbers are located near the each lab exit door)
24. Don’t work alone in the lab
• In case of a
problem, you may
need another
person to prevent
injury or even
save your life!
25. Remember that the lab is a
place for serious work!
Careless behavior may endanger
yourself and others and will not be
tolerated!
26. Demonstrate safe behavior
• Obey all safety instructions.
• Clean up spills immediately; IF
you know how. If you are
uncertain how to clean up a
spill or if a large spill occurs,
notify persons in accordance
to the emergency procedure.
29. Demonstrate safe behavior
• Before leaving the lab:
– Return equipment and chemicals to
their proper places
– Be sure to replace the lids to all
containers
30. Demonstrate safe behavior
• Before leaving the lab:
– Return equipment and chemicals to
their proper places
– Be sure to replace the lids to all
containers
– Clean up your work area
31. Know how to dispose of waste
• Dispose of all waste materials
according to your instructional
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