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- 1. Laboratory safety Tapeshwar Yadav (Lecturer) BMLT,DNHE, M.Sc. Medical Biochemistry
- 2. Introduction Laboratory safety rulesare a major aspect of every clinical lab. Each student in clinical laboratory must follow specific safety rules and procedures.
- 3. Why is LabSafety Important? Lab safety rules and symbols are needed so that students do not injure themselves or their classmates.
- 4.
- 5. Lab Safety Rules 1-Wearprotective clothing .
- 6. Protective clothes o Glovesare essential. o Lab coats are required. o Safety glasses ( goggles) may be required to avoid splashes.
- 7. 2-Laboratory personnel shouldnot wear sandals Lab Safety Rules
- 8. Lab Safety Rules DoNOT Wear: • Jewelry • Loose or Baggy clothing
- 9. 3-Avoid touching objects(e.g., pencils, cell phones, door handles) while wearing gloves. Lab Safety Rules
- 10. 4-Pencils, labels, orany other materials should never be placed in your mouth.
- 11. 5-Caution must betaken when using gas burners. Be sure gas burners are turned off when finished.
- 12. 6-Long hair mustbe tied back or covered to minimize fire hazard or contamination of experiments. Lab Safety Rules
- 13. Lab Safety Rules 7-Do not eat food or drink water in the lab. do not use lab glassware as food or water containers.
- 14. Lab Safety Rules 8-Protect your hands safety: - wash hands after every lab. - Handle glassware, sharp tools and heated containers carefully.
- 15. Lab Safety Rules 9-electrical safety: - unplug electrical equipment after use. - keep all electrical cords and wires away from water .
- 16. Lab Safety Rules 10-Chemical safety: -never touch, taste or smell a chemical unless instructed to do so. - never mix chemicals unless instructed to do so. -keep lids on chemical containers when not in use.
- 17. Lab Safety Rules 11-Donot take any cultures out of the lab for any reason. All cultures should be handled as potentially pathogenic. Liquid cultures must always be kept in a test tube rack.
- 18. Lab Safety Rules 12-Do not engage in practical jokes or horseplay in the lab.
- 19. Lab Safety Rules 13-Keepnonessential books and clothing far away from your work area.
- 20. Lab Safety Rules 14-Wipethe bench tops down with disinfectant both before you begin your work and after you have completed your work.
- 21. Lab Safety Rules 15-Dispose of waste products according to instructions.
- 22. 16-Report all accidents,no matter how minor, to your supervisor!! Lab Safety Rules
- 23.
- 24. Lab Safety Equipment Safetyshower Eye wash
- 25. Lab Safety Equipment FireBlanket Fire Extinguisher
- 26.
- 27. Lab Safety Symbols •They alert about the possible dangers in the lab.
- 28.
- 29. Biological Safety Levels 1.BSL1 2. BSL2 3. BSL3 4. BSL4
- 30. BSL-1 – Not knownto cause disease in healthy adult humans • Safety equipment – Minimal requirements • Facilities – Open bench top
- 31. BSL-2 – Associated withmild to moderate disease in humans • Safety equipment – Biological Safety Cabinet and personal protective equipment as needed. • Facilities – BSL-1 plus the availability of a mechanism for decontamination.
- 32. BSL-3 – Associated withserious or potentially lethal disease in humans • Safety equipment – Biological Safety Cabinet and personal protective equipment required. • Facilities – BSL-2 with self-closing double door access and single-pass negative directional airflow.
- 33. BSL-4 – Associated withhigh risk of life- threatening disease in humans and/or animals • Safety equipment – Biological Safety Cabinet – Full-body air-supplied, positive pressure personnel suit • Facilities – BSL-3 plus dedicated air and exhaust, decontamination procedures for exit, separate building, etc.