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UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS
International General Certificate of Secondary Education

*5439306249*

0610/63

BIOLOGY
Paper 6 Alternative to Practical

May/June 2013
1 hour

Candidates answer on the Question Paper.
No Additional Materials are required.
READ THESE INSTRUCTIONS FIRST
Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use a pencil for any diagrams or graphs.
Do not use staples, paper clips, highlighters, glue or correction fluid.
DO NOT WRITE IN ANY BARCODES.
Answer all questions.
Electronic calculators may be used.
You may lose marks if you do not show your working or if you do not use appropriate units.
At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

This document consists of 13 printed pages and 3 blank pages.
IB13 06_0610_63/4RP
© UCLES 2013

[Turn over
2
1

(a) Some students investigated the effect of enzyme concentration on starch.
They were provided with a Petri dish containing a layer of starch agar jelly.
The students cut three small holes in the starch agar jelly and labelled them as shown
in Fig. 1.1.
Petri dish

starch agar jelly

P

Q

R

hole in the
starch agar jelly

Fig. 1.1
They placed the starch agar jelly that had been cut out of the holes on a white tile and
added two drops of dilute iodine solution to each piece.
The dilute iodine solution changed from yellow/brown to deep blue.
(i) State why the colour changed.

[1]
The students were given two different concentrations of the same enzyme,
solution 1 and solution 2.
•
•
•
•

© UCLES 2013

They put two drops of enzyme solution 1 into hole P.
They put two drops of enzyme solution 2 into hole Q.
They put two drops of water into hole R.
After 15 minutes dilute iodine solution was poured over the surface of the
starch agar jelly.

0610/63/M/J/13

For
Examiner's
Use
3
Fig. 1.2 shows the appearance of the surface of the starch agar jelly after dilute iodine
solution had been added. The Petri dish was placed on a piece of squared paper.

For
Examiner's
Use

P
key
deep blue
clear

R

hole in the
starch agar
jelly

Q

1 small square = 4mm 2
= 4mm 2

Fig. 1.2
(ii) Holes P, Q and R are the same size.
Use the grid and count the squares to estimate the area of hole R.

area of hole R

© UCLES 2013

0610/63/M/J/13

mm2

[1]

[Turn over
4
(iii) Describe the results shown in Fig. 1.2.

For
Examiner's
Use

[3]
(iv) Explain the results shown in Fig. 1.2.

[3]
(v) Suggest the name of the enzyme used in this investigation.
[1]
(vi) State why water was added to hole R.

[1]

© UCLES 2013

0610/63/M/J/13
5
(b) Germinating seeds produce enzymes that change stored food into soluble materials.
Suggest a method similar to that in (a) that you would use to find out if germinating pea
seeds produce the same enzyme as in enzyme solutions 1 and 2.

For
Examiner's
Use

[4]

© UCLES 2013

0610/63/M/J/13

[Turn over
6
(c) Fig. 1.3 shows a pea seedling.

For
Examiner's
Use

Fig. 1.3
Make a large, labelled drawing of the pea seedling in the space provided on page 7.

© UCLES 2013

0610/63/M/J/13
7
Draw the pea seedling in the space below.

[4]

© UCLES 2013

0610/63/M/J/13

[Turn over
8
(d) Fig. 1.4 shows pea seeds in a pod.
pea seeds

pod

Fig. 1.4
The number of pea seeds in a pod varies.
Two students picked a sample of 23 pods.
They opened the pods and counted the number of pea seeds.
Fig. 1.5 shows the students’ results.

number of pea seeds in each pod
8, 10, 11, 10, 9, 11, 9, 4,
10, 11, 12, 10, 10, 11, 8, 12,
10, 9, 11, 8, 10, 9, 12

Fig. 1.5

© UCLES 2013

0610/63/M/J/13

For
Examiner's
Use
9
(i) Complete Table 1.1 using the results from Fig. 1.5 to show how many pods there
were with each number of pea seeds.
Two rows have been completed for you.

For
Examiner's
Use

Table 1.1
number of pea seeds in
each pod

tally

number of pods

///

3

//// //

7

4
5
6
7
8
9
10
11
12
[2]
(ii) Draw a histogram on Fig. 1.6 to show the number of pods with each number of pea
seeds.

[4]

Fig. 1.6
(iii) Put an X in the bar on the graph which seems to be anomalous.

© UCLES 2013

0610/63/M/J/13

[1]

[Turn over
10
(iv) Most pods contained 10 or 11 pea seeds.

For
Examiner's
Use

Suggest a reason for some pods containing 8 or 12 pea seeds.

[1]
[Total: 26]
2

Fig. 2.1 shows an arthropod.

S

T

× 2.5

Fig. 2.1
(a) You are going to calculate the actual length of the part of the leg that is marked ST in
Fig. 2.1.
Measure the length of line ST.

length of line ST

mm

Calculate the actual length of the part of the leg that is marked ST.
Show your working.

actual length of leg

© UCLES 2013

0610/63/M/J/13

mm

[3]
11
(b) Use features, visible in Fig. 2.1, to identify the group of arthropods to which this animal
belongs.

For
Examiner's
Use

Give two reasons for your answer.
Group
reason 1

reason 2
[3]
[Total: 6]

© UCLES 2013

0610/63/M/J/13

[Turn over
12
3

(a) Fig. 3.1 shows a section of a dicotyledonous root as seen with a microscope.

For
Examiner's
Use

Fig. 3.1
On Fig. 3.1:
draw a line to a root hair cell and label it;
draw a line to a cortex cell and label it.

© UCLES 2013

0610/63/M/J/13

[2]
13
(b) When stems have just been cut, drops of liquid often appear on the cut surface of the
stem.

For
Examiner's
Use

A dicotyledonous stem was cut and the liquid was collected and tested for:
•
•
•
•

water;
reducing sugar;
protein;
fat.

The results are shown in Table 3.1.
Complete Table 3.1 to show the reagents and final colours.
Table 3.1
results
substance

reagent
initial colour

water

cobalt chloride

final colour

positive or
negative
( or )

blue

reducing sugar

blue

protein

blue

fat

ethanol + water

colourless
[6]
[Total: 8]

© UCLES 2013

0610/63/M/J/13

[Turn over
14
BLANK PAGE

© UCLES 2013

0610/63/M/J/13
15
BLANK PAGE

© UCLES 2013

0610/63/M/J/13
16
BLANK PAGE

Copyright Acknowledgements:
Question 3 Fig. 3.1

© Ref: C003 / 4134; Broad bean root, light micrograph; Dr Keith Wheeler, Science Photo Library.

Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every
reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the
publisher will be pleased to make amends at the earliest possible opportunity.
Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge
Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge.

© UCLES 2013

0610/63/M/J/13

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0610 s13 qp_63

  • 1. UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS International General Certificate of Secondary Education *5439306249* 0610/63 BIOLOGY Paper 6 Alternative to Practical May/June 2013 1 hour Candidates answer on the Question Paper. No Additional Materials are required. READ THESE INSTRUCTIONS FIRST Write your Centre number, candidate number and name on all the work you hand in. Write in dark blue or black pen. You may use a pencil for any diagrams or graphs. Do not use staples, paper clips, highlighters, glue or correction fluid. DO NOT WRITE IN ANY BARCODES. Answer all questions. Electronic calculators may be used. You may lose marks if you do not show your working or if you do not use appropriate units. At the end of the examination, fasten all your work securely together. The number of marks is given in brackets [ ] at the end of each question or part question. This document consists of 13 printed pages and 3 blank pages. IB13 06_0610_63/4RP © UCLES 2013 [Turn over
  • 2. 2 1 (a) Some students investigated the effect of enzyme concentration on starch. They were provided with a Petri dish containing a layer of starch agar jelly. The students cut three small holes in the starch agar jelly and labelled them as shown in Fig. 1.1. Petri dish starch agar jelly P Q R hole in the starch agar jelly Fig. 1.1 They placed the starch agar jelly that had been cut out of the holes on a white tile and added two drops of dilute iodine solution to each piece. The dilute iodine solution changed from yellow/brown to deep blue. (i) State why the colour changed. [1] The students were given two different concentrations of the same enzyme, solution 1 and solution 2. • • • • © UCLES 2013 They put two drops of enzyme solution 1 into hole P. They put two drops of enzyme solution 2 into hole Q. They put two drops of water into hole R. After 15 minutes dilute iodine solution was poured over the surface of the starch agar jelly. 0610/63/M/J/13 For Examiner's Use
  • 3. 3 Fig. 1.2 shows the appearance of the surface of the starch agar jelly after dilute iodine solution had been added. The Petri dish was placed on a piece of squared paper. For Examiner's Use P key deep blue clear R hole in the starch agar jelly Q 1 small square = 4mm 2 = 4mm 2 Fig. 1.2 (ii) Holes P, Q and R are the same size. Use the grid and count the squares to estimate the area of hole R. area of hole R © UCLES 2013 0610/63/M/J/13 mm2 [1] [Turn over
  • 4. 4 (iii) Describe the results shown in Fig. 1.2. For Examiner's Use [3] (iv) Explain the results shown in Fig. 1.2. [3] (v) Suggest the name of the enzyme used in this investigation. [1] (vi) State why water was added to hole R. [1] © UCLES 2013 0610/63/M/J/13
  • 5. 5 (b) Germinating seeds produce enzymes that change stored food into soluble materials. Suggest a method similar to that in (a) that you would use to find out if germinating pea seeds produce the same enzyme as in enzyme solutions 1 and 2. For Examiner's Use [4] © UCLES 2013 0610/63/M/J/13 [Turn over
  • 6. 6 (c) Fig. 1.3 shows a pea seedling. For Examiner's Use Fig. 1.3 Make a large, labelled drawing of the pea seedling in the space provided on page 7. © UCLES 2013 0610/63/M/J/13
  • 7. 7 Draw the pea seedling in the space below. [4] © UCLES 2013 0610/63/M/J/13 [Turn over
  • 8. 8 (d) Fig. 1.4 shows pea seeds in a pod. pea seeds pod Fig. 1.4 The number of pea seeds in a pod varies. Two students picked a sample of 23 pods. They opened the pods and counted the number of pea seeds. Fig. 1.5 shows the students’ results. number of pea seeds in each pod 8, 10, 11, 10, 9, 11, 9, 4, 10, 11, 12, 10, 10, 11, 8, 12, 10, 9, 11, 8, 10, 9, 12 Fig. 1.5 © UCLES 2013 0610/63/M/J/13 For Examiner's Use
  • 9. 9 (i) Complete Table 1.1 using the results from Fig. 1.5 to show how many pods there were with each number of pea seeds. Two rows have been completed for you. For Examiner's Use Table 1.1 number of pea seeds in each pod tally number of pods /// 3 //// // 7 4 5 6 7 8 9 10 11 12 [2] (ii) Draw a histogram on Fig. 1.6 to show the number of pods with each number of pea seeds. [4] Fig. 1.6 (iii) Put an X in the bar on the graph which seems to be anomalous. © UCLES 2013 0610/63/M/J/13 [1] [Turn over
  • 10. 10 (iv) Most pods contained 10 or 11 pea seeds. For Examiner's Use Suggest a reason for some pods containing 8 or 12 pea seeds. [1] [Total: 26] 2 Fig. 2.1 shows an arthropod. S T × 2.5 Fig. 2.1 (a) You are going to calculate the actual length of the part of the leg that is marked ST in Fig. 2.1. Measure the length of line ST. length of line ST mm Calculate the actual length of the part of the leg that is marked ST. Show your working. actual length of leg © UCLES 2013 0610/63/M/J/13 mm [3]
  • 11. 11 (b) Use features, visible in Fig. 2.1, to identify the group of arthropods to which this animal belongs. For Examiner's Use Give two reasons for your answer. Group reason 1 reason 2 [3] [Total: 6] © UCLES 2013 0610/63/M/J/13 [Turn over
  • 12. 12 3 (a) Fig. 3.1 shows a section of a dicotyledonous root as seen with a microscope. For Examiner's Use Fig. 3.1 On Fig. 3.1: draw a line to a root hair cell and label it; draw a line to a cortex cell and label it. © UCLES 2013 0610/63/M/J/13 [2]
  • 13. 13 (b) When stems have just been cut, drops of liquid often appear on the cut surface of the stem. For Examiner's Use A dicotyledonous stem was cut and the liquid was collected and tested for: • • • • water; reducing sugar; protein; fat. The results are shown in Table 3.1. Complete Table 3.1 to show the reagents and final colours. Table 3.1 results substance reagent initial colour water cobalt chloride final colour positive or negative ( or ) blue reducing sugar blue protein blue fat ethanol + water colourless [6] [Total: 8] © UCLES 2013 0610/63/M/J/13 [Turn over
  • 14. 14 BLANK PAGE © UCLES 2013 0610/63/M/J/13
  • 15. 15 BLANK PAGE © UCLES 2013 0610/63/M/J/13
  • 16. 16 BLANK PAGE Copyright Acknowledgements: Question 3 Fig. 3.1 © Ref: C003 / 4134; Broad bean root, light micrograph; Dr Keith Wheeler, Science Photo Library. Permission to reproduce items where third-party owned material protected by copyright is included has been sought and cleared where possible. Every reasonable effort has been made by the publisher (UCLES) to trace copyright holders, but if any items requiring clearance have unwittingly been included, the publisher will be pleased to make amends at the earliest possible opportunity. Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of Cambridge Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge. © UCLES 2013 0610/63/M/J/13