2. Outline
1. Introduction
2. Ultrasound principle
3. Physical effect : acoustic cavitation
4. Working
5. Methods of Ultrasound application
6. Applications in food processing
7. Application in Food Preservation
8. Reference
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3. 1. Introduction
Modern food industry is always on the way looking for innovative technologies which
can produce
high-quality and safe products,
enhance the processing efficiency,
and reduce the energy consumption.
Power ultrasound is considered to be an emerging and promising technology for food
processing industry.
Ultrasound makes use of physical and chemical phenomena that are fundamentally
different compared with those applied in conventional processing or preservation
techniques.
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7. 5. Methods of Ultrasound application
Direct application to the product.
Coupling with the device.
Submergence in an ultrasonic bath.
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9. 6.1 Filtration
Conventional filtration have problem of clogging of solids in filter
membrane.
Ultrasound prevent solid deposition by using acoustic cavitation.
Ultrasonically filtration use vibration as a principle.
Ultrasound do not affect the intrinsic permeability of membrane.
10. 6.2 Defoaming
Foam is historically controlled by mechanical breaker, lowering
packaging temperature and the use of chemical anti-foams. (many
drawbacks)
High intensity ultrasound waves break the foam by acoustic
pressure, radiation pressure, bubble resonance or streaming and
liquid film cavitation
Effectively used in controlling the excess foams produce during
canning and bottling lines of beverages.
Two parameters are important viz. acoustic energy and treatment
time.
11. 6.3 Degassing
Degassing is the removal of dissolved gases from the liquid.
Mechanical agitators and boiling has some drawbacks.
Ultrasound processing can easily deaerate the liquid by bring bubbles to
the surface and finally burst with a small temperature change.
Used to degas carbonated beverages such as beer before bottling.
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12. 6.5 Demoulding
Industrial cooking of food leads to the adhesion of the product to the
cooking vessel and it is difficult to remove.
Moulds are fabricated with the coating of silicon or PTFE but these
have shorter self life and are also expensive.
Ultrasound can effectively use for demoulding by coupling the
source of ultrasound with mould.
It also ensure no residual material left in the cooking vessel.
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13. 6.6 Cutting
Ultrasonic food cutting equipment provide a new way to cut or slice a
variety of food products that minimizes product waste.
Ultrasound food cutting uses a knife slide blade attached through a shaft
to the ultrasonic source.
Ultrasound food cutting has advantage of hygienic cutting.
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14. Cutting difference in cutting slices of a bakery product
cut with and without ultrasound.
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15. 6.7 Freezing and Crystallization
The problems related to conventional freezing is non-uniform crystal development,
destruction of food material structure and loss in sensory food quality.
Sonication is thought to enhance both the nucleation rate and rate of crystal growth in a
saturated or super cooled medium.
With the influence of ultrasound, conventional cooling will be much faster.
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16. 6.8 Thawing
Thawing using conventional method by generating heat is a fast process
but there is always chance of loss of flavour, colour, etc.
Utilization of acoustic energy, thawing under the relaxation frequency
range is faster than the thawing using conductive heating only.
Acoustic thawing shorten the thawing time which result in the reduction
of drip loss and improve product quality.
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17. 6.9 Drying
Traditional method of drying takes longer time to eliminate the interior
moisture content and increase temperature cause change in colour, taste,
nutritional value of food.
Ultrasound field + hot gas stream = fast drying.
Ultrasonic osmotic dehydration technology uses lower temperature thus
saved colour, flavour and nutritional value of the food.
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18. 6.10 Emulsification
Acoustic emulsification has particle in sub-micro range.
Emulsions are more stable.
No use surfactant to stabilize the emulsion.
Energy used by acoustic waves is less than the conventional method.
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20. 7.1 Introduction
High-intensity ultrasonic waves can rupture cells and denature enzymes, and even
low-intensity ultrasound is able to modify the metabolism of cells.
In combination with heat, ultra-sonication can accelerate the rate of sterilization of
foods, thus lessening both the duration and intensity of thermal treatment and the
resultant damage.
The advantages of ultrasound over heat sterilization includes the minimizing of
flavour loss, greater homogeneity and significant energy savings.
The mechanism of microbial killing is mainly due to the thinning of cell membranes,
localized heating and production of free radicals.
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21. 7.2 Principle
High temperature and shear stress can rupture the cell membrane.
In transient cavitation the growth and collapse of bubble are violent which led to
violent jet of liquid causes rupture of cell wall.
In stable cavitation, low amplitude and high frequency waves causes only
oscillation of bubbles and induce micro-streaming in liquid which causes stress in
microorganism.
Intercellular cavitation can disrupt cellular structure and functional components up
to the point of cell lysis.
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23. 7.4 Factors affect the microbial inactivation
Amplitude of ultrasound waves.
Frequency of ultrasound waves.
Exposure or contact time.
Volume of food processed.
Treatment temperature.
Type, shape or diameter of the micro-organisms.
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24. 7.5 Can ultrasound alone effective to inactivate micro-
organism?
Manosonication: combination of ultrasound and pressure (MS).
Thermosonication: combination of ultrasound and heat (TS).
Manothermosonication: combination of ultrasound, pressure and
heat (MTS).
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25. 7.6 Effect on Saccaromyces cerevisiae
Treatment of yeast in water at 45 C.
Using ultrasound treatment alone.
At a power of 5 KW, the ultrasound waves didn’t increase the sensitivity of
Saccaromyces cerevisiae cell to heat.
At a power of 180 KW sensitizing action due to ultrasound is visible but not so
much.
At a power of 100 KW, the sensitivity of cell to heat increase drastically.
25
26. 7.7 Values of decimal reduction time for the various
treatments of Saccharomyces cerevisiae
26
Treatment D (50 C) D (55 c)
control 16 min 4.3 min
Pact= 50 KW 16 min 4.3 min
Pact = 100 KW 9 min 1.6 min
Pact = 150 KW 15 min 2.4 min
27. 7.8 Effect of ultrasound with temperature change
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28. 8.0 References
A. Scotto, Device for demoulding industrial food products, Fr. Pat. FR 2
604 063, 1988.
Feng Hao, Barbosa-Cánovas Gustavo V., and Weiss Jochen, Ultrasound
Technologies for Food and Bioprocessing,(2010), 321-340
Chemat Farid , Zill-e-Huma, and Kamran Khan Muhammed, Journal of
Applications of ultrasound in food technology: Processing, preservation
and extraction.(2010)
Abbas Shabbar ,Hayat Khizar ,Karangwa Eric,Bashari Mohanad and
Zhang Xiaoming, Journal of An Overview of Ultrasound-Assisted Food-
Grade Nanoemulsions.(2013) 3/17/2018
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Ultrasound waves, when pass through a liquid medium; induce molecular motion through a series of compression and rarefaction cycles. Preexisting micro-bubbles of gas dissolved into the liquid medium grow in size due to
rectified diffusion with each succeeding expansion and rarefaction half-cycle until they become unstable and violently collapse [89–91] (Fig. 3). Rectified diffusion is phenomena of bubble growth under the influence of increased gas exchange area and larger inward flux during expansion [92]. Briefly, acoustic cavitation is a ‘‘growth and collapse of micro-bubbles under an ultrasonic field’’ [93] as quoted by Ashokkumar [89]. Negative acoustic pressure (Fig. 3) in the liquid medium induces formation of cavitation bubbles. Furthermore, vibration amplitude (see Fig. 3) directly influences the sonication intensity as an increment in the vibration amplitude leads to greater vibration intensity, consequently increasing the cavitation effects. Physical effects produced by acoustic cavitation, that is, shock waves, micro-jets, turbulence, shear forces, temperature, etc., can be employed for a number of applications, including cleaning, extraction, inactivation, modification and emulsification. Applications of ultrasound technology for food processing were a topic of several
reviews [62, 65, 94–102] and book chapters [74, 80, 86, 103, 104] published in the last few years
Like any sound wave, ultrasound is propagated via a series of compression and rarefaction waves induced in the molecules of the medium through which it passes.
At sufficiently high power the rarefaction cycle forms low pressure condition which forms the bubble.
At low frequencies of 20–30 kHz, a smaller number of cavitations with larger sizes and higher energy are generated. Much denser cavitations with moderate or lower energies are formed as frequency increases.
The ultrasonic cleaning illustration (Fig. 21.3) shows the generating cavitation through at least three steps: nucleation, growth and violent collapse, or implosion. The transient cavities (or vacuum bubbles or vapor voids), ranging from 50 to 150 μmindiameterat25kHz,areproducedduringthesoundwaves’halfcycles.During the rarefaction phase of the sound wave, the liquid molecules are extended outward against and beyond the liquid’s natural physical elasticity/bonding/attraction forces, generating vacuum nuclei that continue to grow to a maximum. Then, violent collapse occurs during the compression phase of the wave. It is believed that the latter phase is augmented by the enthalpy of the medium and the degree of mobility of the molecules, as well as the hydrostatic pressure of the medium.
Cavitations are generated in the order of microseconds. At 20 kHz frequency, it is estimated that the pressure is about 35–70 kPa and the transient localized temperatures are about 5,000◦C, with the velocity of micro-streaming around 400 km/h (Table 21.1). Several factors have great influence on the cavitation intensity and abundance in a given medium.
When the local pressure is decreased sufficiently below the vapour pressure during the rarefaction period of the sound wave, the static pressure and the cohesive forces are overcome and gas bubbles are formed. They will generally oscillate, grow, and then collapse violently [19, 20
A unique phenomenon takes place when high-energy ultrasonic waves [20 kHz to about500kHz(atabout0.3–1W/cm2)]travelinaliquidorinasolution.Thewaves interact with the liquid medium to generate a highly dynamic agitated solution. In the process, high-intensity ultrasonic waves create micro-vapor/vacuum bubbles in theliquidmedium,whichgrowtoamaximumsizeproportionaltotheappliedultrasonic frequency and then implode, releasing their energies. This phenomenon is known as cavitation implosion. The higher the frequency, the smaller the cavity size, with lower implosion energy. The high-intensity ultrasonics can grow cavities to a maximum in the course of a single cycle. At 20 kHz, the bubble size is roughly 170 μm in diameter (Fig. 21.1). At a higher frequency of 68 kHz, the total time from nucleation to implosion is estimated to be about one-third of that at 25 kHz. The vacuum bubble size becomes smaller at higher frequencies as a function of the wavelength. For example, at 132 kHz the bubble size is estimated to be about half of that generated at 68 kHz, and the size will be even much smaller at 200 kHz.
Meanwhile, at higher frequencies, the minimum amount of energy required to produceultrasoniccavitiesishigherandmustbeabovethecavitationthreshold.Inother words, the ultrasonic waves must have enough pressure amplitude to overcome the natural molecular bonding forces and the natural elasticity of the liquid medium in order to grow the cavities. For water, at ambient temperature, the minimum amount ofenergyneededtobeabovethethresholdwasfoundtobeabout0.3and0.5W/cm2 pertankradiatingsurfacefor20and40kHz,respectively. Theenergyreleasedfrom implosions is expressed in new forces, namely shock wave, micro-streaming, shear forces, and miniature eddy currents.
The transducers most commonly used for generating ultrasonic vibrations are piezoelectric, magnetostrictive, electromagnetic, pneumatic, and other mechanical devices. The piezoelectric transducer (PZT) assembly (Fig. 21.2) is the most widely used configuration in cleaning
Ultrasonic setup a batch-type ultrasonic setup, b components of batch-type ultrasonic setup, that is, generator, transducer, amplifiers and probe-types, c sequence of energy transformations at different levels of the operation
In 2nd =Compared with mechanical agitation, the ultrasonic method decreases the number of broken bottles and overflow of the bever-age .
In 3rd point ==Degas-sing in an ultrasonic field is a highly visible phenomenon when ultrasound, e.g. an ultrasonic cleaning bath, is used with regular tap-water inside. It occurs when the rapid vibration of gas bubbles brought them together by acoustic waves and bubbles grow to a size sufficiently large to allow them to rise up through the liquid, against gravity, until they reach the surface [23,24]. Several acous-tic cavitation structures generated in low-frequency ultrasound fields within the range (20–50 kHz) have been investigated and these have been summarized by Mettin [25].
The ultrasonic cleaning illustration (Fig. 21.3) shows the generating cavitation through at least three steps: nucleation, growth and violent collapse, or implosion. The transient cavities (or vacuum bubbles or vapor voids), ranging from 50 to 150 μmindiameterat25kHz,areproducedduringthesoundwaves’halfcycles.During the rarefaction phase of the sound wave, the liquid molecules are extended outward against and beyond the liquid’s natural physical elasticity/bonding/attraction forces, generating vacuum nuclei that continue to grow to a maximum. Then, violent collapse occurs during the compression phase of the wave. It is believed that the latter phase is augmented by the enthalpy of the medium and the degree of mobility of the molecules, as well as the hydrostatic pressure of the medium.
Cavitations are generated in the order of microseconds. At 20 kHz frequency, it is estimated that the pressure is about 35–70 kPa and the transient localized temperatures are about 5,000◦C, with the velocity of micro-streaming around 400 km/h (Table 21.1). Several factors have great influence on the cavitation intensity and abundance in a given medium.
Frequencies of 20–35 kHz are more appropriate for cleaning heavy and large size components
The energy released from an implosion in close vicinity to the surface collides with andfragmentsordisintegratesthecontaminants
. The implosion also produces dynamic pressure waves, which carry the fragments away from the surface. The implosion is also accompanied by high-speed micro-streaming currents of the liquid molecules. The cumulative effect of millions of continuous tiny implosions in a liquid medium providesthenecessarymechanicalenergytobreakphysicallybondedcontaminants,
Ultrasonic waves generate and evenly distribute cavitation implosions in a liquid medium. The released energies reach and penetrate deep into crevices, blind holes, and areas that are inaccessible to other cleaning methods (Fuchs, 1997; O’Donoghue, 1984). The removal of contaminants is consistent and uniform, regardless of the complexity and the geometry of the substrates
The general ultrasonic power requirements for almost all cleaning applications, expressed in terms of electrical-input wattage to the transducers, is in the range of 7.15–9.15 W/cm2 of transducer radiating surface.
Freezing and crystallization are linked in that both processes in-volve initial nucleation followed by crystallization [54]. Sonication is thought to enhance both the nucleation rate and rate of crystal growth in a saturated or supercooled medium by producing a large number of nucleation sites in the medium throughout the ultra-sonic exposure. This may be due to cavitation bubbles acting as nu-clei for crystal growth and/or by the disruption of seeds or crystals already present within the medium thus increasing the number of nucleation sites.
As is well known, the rate of freezing largely affects the size and distribution of ice crystals; high freezing rates lead to the production of small crystals evenly distributed throughout the tissue, while slow freezing rates generally cause large ice crystals to form exclusively in extracellular areas
Power ultrasound, in particular, has proved to be extremely useful in crystallization processes (Mason, 1998). Controlled crystallization of sugar solutions, hardening of fats, and the manufacture of chocolate and margarine are examples of food processes that can be improved by the application of power ultrasound (Leadley and Williams, 2006). Although the potential of power ultrasound to assist food freezing is promising,
Larger cells are more sensitive than the small ones. This is probably due to their larger surface area. Gram-positive bacteria are known to be more resistant than gram-negative ones, possibly because of their thicker cell wall which provides them a better protection against ultrasound effects .
Concerning the shape of the micro-organisms, cocci are more resistant than bacilli due to the relationship of cell surface and volume. Finally, spores are very hard to destroy compared to vegeta-tive cells which are in phase of growth
main difference between cocci and bacilli is that cocci are spherical or oval-shaped bacteria whereas bacilli are rod-shaped bacteria.
At a power of 5 KW, the intensity of ultrasonic wave is too low the suspension is too low to allow a good development of cavitation. This leads to the formation of a smaller number of bubbles, and to vibrations of smaller amplitudes. The level of damage of the yeast is not sufficient to increase the sensi-
Here the ultrasound didn’t break the cells into fragments but cause the puncturing of cell wall and cell membrane which causes more sensitivity of micro organism towards heat.
No decrease in microbial population.
Damage cell wall, cytoplasmic membrane but no cell rupture.
Also, solid particles placed in a turbulent field are sur- rounded by a layer where streaming is stationary. This layer is a barrier to heat transfer during heat treatment. Micros- treaming generated by acoustic cavitation affects this layer which becomes thinner, and thus heat transfer from the sus- pension to the centre of the cell is accelerated, resulting in faster cellular deactivation and destruction. Such a phenom- enon also explains the more pronounced destructive effect of ultrasound at higher temperatures.