It is a research paper which talks about Mimicking the melanin production to help in coloring the human hair. The paper has been published in ACS journal(2017).
2. COLOR OF HUMAN HAIR
■ Pigmentation of hair follicles - MELANIN.
■ Types of pigmentation –
1. Eumelanin (Responsible for dark shades)
2. Pheomelanin (Responsible for lighter shades)
4. BIRTH OF HAIR DYES!!!
■ PPDA and related diamine based monomeric compounds.
■ Oxidising agent – Hydrogen peroxide
Before coloring Lift due to Ammonia Follicle ready for coloring polymerisation
5. ■ PPDA - allergic and carcinogenic in nature.
■ It has a MW less than 500 Da
■ Hydrogen peroxide bleaches our hair
■ Ammonia – Damages the outer part of the hair
eumelanin
6. What are the safest dyeing agents for human grey hair?
How about ammonia free
or PPDA free products???
How about natural
products like Henna??
How about melanin ???
Melanin aggregates
9. Structural difference
• The presence of catechol
group in Dopamine
makes it more more
adhesive in nature.
• The approach used here
is ‘bottom-up’ manner
MELANIN
EUMELANIN
DOPAMINE
CHELATIONHYDROGEN BONDING
10. ■ Iron(III) sulfate n-hydrate
■ Iron(II) sulfate heptahydrate
■ Copper(II) chloride
■ Aluminum(III) potassium sulfate
■ Dopamine hydrochloride
0.4g of dopamine HCL into tris-HCL buffer
(pH 8.1)
Incubation for 6hrs for prepolymerization
soaked in a volume of 6 mL of the 6 h
incubated solution for 1 hr
0.33 g of iron(II)
sulfate heptahydrate is
added
The dyed hair was washed with tap water
And dried
Conventional colorimeter
The dyed hair was washed with SDS
12. A. Color difference value of virgin
& dyed hair (before SDS wash)
B. Color difference value of dyed
hair with SDS
C. SEM pictures before SDS wash
D. Photographical images of
virgin and dyed hairs before
SDS treatments.
13. • Different shades can be
obtained by chelating
polydopamine with
different metals
14. A) Color difference value of dyed hair with ferrous ion during different incubation time.
B) SEM images at different incubation time.
C) IR spectra –comparison at different incubation time.
A B
C
15. A) Fe content of hair.
B) EPR spectra revelling the presence of Fe-complex.
A B
16. TOXICITY TEST ON
MICE
The low concentration of PPDA and
PD+Fe have been subjected to 5 mice
each and the high concentrations where
subjected to 7 mice each
The death rate of PPDA subjected mice
has been found to be more.
17. CONCLUSION
■ Novel method of dyeing hair that are safer and more eco-friendly.
■ Treatment was accomplished within a commercially acceptable period of time.
■ Wide range of hue can be produced by varying metal ions.
■ Good color retension.