this PPT is about Agrobacterium, its role in infection and used as a tool for gene transfer in case of plants. the significance of agrobacterium is also included in this presentation.

1. Induction of Hairy Roots by Agrobacterium
rhizogenes
Wounded plant cells
Signal Molecules
Recognition by
Agrobacterium
Attachemnt of
Agrobacterium With plant cells
Transfer of Ri plasmid to
wounded plant cells
Co-Cultivation
Integration of Ri plasmid
into plant genome
Hairy Root Induction
Transfer of
Ti/Ri Plasmind
in plant cell
/rhizogenes

2. Hairy root culture

3. Hairy root cultures are characterized by a high
growth rate and are able to synthesize root derived
secondary metabolites.
Normally,root cultures need an exogenous
phytohormone supply and grow very slowly,
resulting in poor or negligible secondary metabolite
synthesis.
However, the use of hairy root cultures has
revolutionized the role of plant tissue culture for
secondary metabolite synthesis.
These hairy roots are unique in their genetic and
biosynthetic stability.
More characteristic of Hairy roots

4. Their fast growth, low doubling time, ease of
maintenance, and ability to synthesize a range of
chemical compounds offers an additional
advantage as a continuous source for the
production of valuable secondary metabolites.
To obtain ahigh-density culture of roots, the
culture conditions should be maintained at the
optimum level.
Hairy root cultures follow a definite growth
pattern, however, the metabolite production may
not be growth related.