How Same-Day Salmonella Quantification Guides Intervention Strategies and Lowers Costs
•
0 likes•130 views
Hygiena shares ways leading poultry processors are getting their products to market faster and saving costs with game-changing, same-day Salmonella quantification technology.
Recommended
More Related Content
Similar to How Same-Day Salmonella Quantification Guides Intervention Strategies and Lowers Costs
Similar to How Same-Day Salmonella Quantification Guides Intervention Strategies and Lowers Costs (20)
More from SafetyChain Software
More from SafetyChain Software (20)
Recently uploaded
Recently uploaded (20)
How Same-Day Salmonella Quantification Guides Intervention Strategies and Lowers Costs
- 1. 01 June 3, 2020 BEYOND COMPLIANCE Webinar and Podcast Series How Same-Day Salmonella Quantification Guides Intervention Strategies and Decreases Costs Presented by: David Luedeke, M.S. Hygiena June 3, 2020 CONFIDENTIAL AND PROPRIETARY
- 2. The Series 02 Webinar & Podcast Series BEYONDCOMPLIANCE Aaron Bolshaw VP of Marketing SafetyChain Software CONFIDENTIAL AND PROPRIETARY
- 3. Series Sponsor 03 REDUCE RISK Suppliers Receiving Pre-Op Processing OEE/SPC Shipping Audit LOAD ACCURACY INCREASE PRODUCTIVITY IMPROVE YIELD IMPROVE THROUGHPUT REDUCE HOLDS ENSURE COMPLIANCE Plant Management Platform CONFIDENTIAL AND PROPRIETARY
- 4. Before We Get Started 04 ✔ Informal but informational ✔ Ask questions (Q&A at end) ✔ Only panelists are displayed ✔ Recording link will be shared ✔ Audio issues Watch Previous Episodes Online CONFIDENTIAL AND PROPRIETARY
- 5. Our Session Sponsor 05 • ATP monitoring systems • PCR-based foodborne pathogen detection • DNA fingerprint molecular characterization systems • Mycotoxin and allergen tests • Environmental collection devices, and more CONFIDENTIAL AND PROPRIETARY
- 6. Our Presenter and Session Sponsor 06 • Former Key Account Manager with NSF International - managed retail food safety auditing programs • Former Researcher at Battelle Memorial Institute, worked with BSL3 organisms and Select Agents in the laboratory • Task Lead for the Food Model in the Department of Homeland Security’s Bioterrorism and Chemical Terrorism Risk Assessments • Received Masters Degree in Biology from UNC-Wilmington and BA in Biology from Asbury University J. David Luedeke, M.S. Hygiena Territory Manager for the Southeastern US Region David Luedeke CONFIDENTIAL AND PROPRIETARY
- 7. Topic: Same-Day Salmonella Quantification 07CONFIDENTIAL AND PROPRIETARY
- 8. SALMONELLA QUANTIFICATION CONFIDENTIAL AND PROPRIETARY BAX® System Real-Time Salmonella 08
- 9. •Regulations are driving the F&B industry to better understand their intervention effectiveness across the production chain •Currently available enumeration technologies do not provide a route for rapid, actional data to drive decisions in holistic food safety management systems •Providing a reliable, accurate, and precise enumeration method is vital to continue industry improvements in food safety The Need for New Technologies 09CONFIDENTIAL AND PROPRIETARY
- 10. Peace of Mind, Quantified Same Machine, Same Assay, Multiple Applications 010CONFIDENTIAL AND PROPRIETARY
- 11. CURRENT STATE OF TECHNOLOGY •Most Probable Number (MPN) •Agar Plates • Cost Increase per sample • Increase in working sample size • Labor intensive • Increased variability (human error) • Large bench space needed • 9 X Consumables • Slow time to results and calculation 011CONFIDENTIAL AND PROPRIETARY
- 12. •Most Probable Number (MPN) •Agar Plates 1 Lysate Tube 1 BAX® PCR Tube •BAX® System SalQuant CURRENT STATE OF TECHNOLOGY 012CONFIDENTIAL AND PROPRIETARY
- 13. Rinsate Example MPN 1. Serially dilute 1 mL to 0.1 and 0.01 mL 2. Transfer 1 mL into 3 – 9 mL tubes 3. Transfer 1 mL from each dilution tubes into 3 – 9 mL tubes 4. Incubate 9 tubes for 18 – 24 h @ 37°C 5. Transfer incubated solution into selective broths 6. Incubate for 18 – 24 h 7. Plate 1 mL onto selective agar 8. Incubate for 18 – 24 h 9. Confirm typical colonies via PCR or agglutination 10. Utilize confirmed tubes in MLG MPN tables to calculate MPN/mL SALQUANT 1. Aliquot transfer (30 mL) 2. Add Quant media (30 mL) 3. Incubate for 6 h @ 42°C 4. One BAX Run (75 min) 5. Utilize CT value to calculate CFU/mL 3–4Days SameDay WORKFLOW COMPARISON 013CONFIDENTIAL AND PROPRIETARY
- 14. • BAX® Q7 • Real-Time Salmonella Assay • Lysate Prep • Run Time (75 min) SALQUANT • Wide enumerable range • Specific CFU per sample • Positive CT value utilized to calculate LIMITS • Designated upper threshold • No Specific CFU per sample • Positive result indicates exceeding threshold BAX® SYSTEM QUANTIFICATION APPLICATIONS 014CONFIDENTIAL AND PROPRIETARY
- 15. • Over 25,000 samples ran to date on various matrices to generate curves • Hygiena has collaborated with Texas Tech University to develop curves for several of the top poultry producers in the US • Industry- and regulatory agency-driven matrices and research for quantification • Effective statistical parameters for improved accuracy and precision • Meeting and exceeding estimations from current conventional quantification methods BAX® CHANGING THE GOLD STANDARD 015CONFIDENTIAL AND PROPRIETARY
- 16. STEP 1 STEP 2 STEP 3 STEP 4 THE DETAILS 016CONFIDENTIAL AND PROPRIETARY
- 17. LIVE PRODUCTION Boot Swabs Ceca Whole Viscera Packs Feed Samples PROCESSING Whole Bird Rinsates Parts Rinsates Chicken Skin FINAL PRODUCT Ground Poultry CONFIDENTIAL AND PROPRIETARY INDUSTRIAL APPLICATIONS 017CONFIDENTIAL AND PROPRIETARY
- 18. PROBLEM Unknown amount of Salmonella entering processing chain from live birds SOLUTION Utilize SalQuant to quantify boot swabs or ceca to provide internal potential load of Salmonella in flock: apply pre-harvest intervention or adjust/monitor interventions during processing INDUSTRY APPLICATIONS: FARM 018CONFIDENTIAL AND PROPRIETARY
- 19. EXPERIMENTAL DESIGN • 50 Positive Chicken Ceca samples • 3 × 8 mMPN per ceca ENRICHMENT PARAMETERS • 1 ceca + 10 mL BPW • 1 mL slurry in 9 mL of BAX MP + Antibiotic • 8 h incubation OUTCOME • Equivalency between methods • Rapid results to implement pre-harvest interventions and evaluating on-farm protocols INDUSTRY APPLICATIONS: FARM 019CONFIDENTIAL AND PROPRIETARY
- 20. EXPERIMENTAL DESIGN • 5 samples per processing location • Prevalence and SalQuant ENRICHMENT PARAMETERS • 30 mL Rinsate • 30 mL BAX MP + Antibiotic • 6 h incubation OUTCOME • Increased prevalence rates from Rehang to Post-Evis, however, Salmonella loads reduced from Rehang to Post-Evis • Increased resolution into processing chain INDUSTRY APPLICATIONS: PROCESSING 020CONFIDENTIAL AND PROPRIETARY
- 21. • Over 25,000 samples ran to date on various matrices to generate curves • Continuous growth of available matrices across processing chain • Effective statistical parameters for improved accuracy and precision • Meeting and exceeding estimations compared to conventional quantification methods • Rapid, actional data to drive decisions in holistic food safety management systems • Providing a reliable, accurate, and precise enumeration method to continue industry improvements in food safety KEY TAKEAWAYS 021CONFIDENTIAL AND PROPRIETARY
- 22. www.hygiena.com/salquant WANT TO STAY INFORMED ABOUT FUTURE RESEARCH? 022CONFIDENTIAL AND PROPRIETARY
- 23. Q & A 023CONFIDENTIAL AND PROPRIETARY
- 24. Resources White Papers & Surveys Webinars & Videos Product & Partner Info Solution Consultation info@safetychain.com SafetyChain.com