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1. Bio Sci D121
STEM CELL BIOLOGY
Lecture 1: Introduction to Stem Cell Biology
4/3/10
Introduction to the course
Info about group presentations
Introduction to stem cell biology
Group discussion
2. Instructor Information
Leslie Lock, Ph.D.
office: Gross Hall 3031
e-mail: llock@uci.edu
Peter Donovan, Ph.D.
office: Gross Hall 3002
e-mail: pdonovan@uci.edu
Alex Stover
e-mail: astover@uci.edu
Office Hours
Dr Lock: Wed 10:00 to 11am Gross Hall, 3031
Dr Donovan: Fri 10:00 to 11am Gross Hall, 3002
Alex Stover: Wed 1:00 to 2 pm BioSci III 2130
Class Website
http://eee.uci.edu/12s/05370
3. Course Information
Meeting Time: Tuesdays & Thursdays 9:30-10:45 am
Regular attendance is expected and advantageous, but not required.
Meeting Place: Nat Sci II Rm 4201
Prerequisites: BIOL 433 with grade of C or better.
Format: Lecture / discussion.
Group presentations & discussions of research papers.
Text: None required.
Recommended: Scott, CT, Stem Cell Now, Penguin Group, 2006
Goldstein, LSB & Schneider, M, Stem Cells for Dummies, 2010
Reference texts: Lanza, R ed., Stem Cell Biology, 2nd ed. Elsevier Pub Co., 2009
Gilbert, S.F., Developmental Biology, 8th ed. Sinauer Pub Co., 2006
Efforts will be made to accommodate special needs. It is the student’s responsibility to
communicate the needs to DR. LOCK as soon as possible in order to make the necessary
arrangements.
You are expected to uphold the university's standards of academic honesty including
plagiarism on all exams.
4. Course Objectives
By the end of this class you should be able to...
Describe the sources, isolation, and characteristics of stem cells.
Compare and contrast totipotent, pluripotent, multipotent, and unipotent stem cells.
Compare and contrast embryonic and tissue-specific (adult) stem cells.
Evaluate uses of stem cells for studying and treating human disease.
Interpret, evaluate, discuss, and present papers from the primary literature.
5. Course Requirements
Grades will be based on the following:
Midterm exam 30%
50% multiple choice
50% short answer
Final exam 40%
25% multiple choice
50% short answer
25% essay
Group Discussions 10%
Key Questions presentations 20%
7. Lecture 1: Introduction to Stem Cell Biology
3/29/11
Introduction to the course
Group discussions and presentations
Introduction to stem cell biology
Group discussion
12. Be Prepared
• Thoroughly understand information you are
presenting before the talk
– Ask someone
• Practice the talk, then practice again
∀ Χηεχκ ψουρ σλιδεσ βεφορε ψου ταλκ
(Check your slides before your talk)
• Know the equipment
• Check video files
Donovan
13. Giving a Presentation
• Start slowly to give the audience time to settle
down and focus on you
• Speak up!
• Talk to the audience not the screen
• Convey your excitement about your proposal
• Remember you are telling a story
• Sometimes less is more
Donovan
14. Frame Your Talk
• Tell people what you are going to tell them
• Tell it to them
• Tell them what you just told them
Donovan
16. Start and finish each slide
• Have a title on each slide
• Summarize the conclusions at the end of each slide
DON’T USE ALL CAPITAL LETTERS
• ALL CAPS IS DIFFICULT TO READ
Donovan
17. Use a font that can be read from the back of the room
Remember, most of the faculty are old and cannot see well
Thompson Med Biochemistry 8/8/2007 10:15 8/8/2007 12:15
Carbohydrates 2 Thompson Med Biochemistry 8/9/2007 10:15 8/9/2007 12:15
Carbohydrates 3 Thompson Med Biochemistry 8/10/2007 10:15 8/10/2007 12:15
Carbohydrates 4 Thompson Med Biochemistry 8/13/2007 9:00 8/13/2007 10:00
Carbohydrates 5 Thompson Med Biochemistry 8/13/2007 10:15 8/13/2007 12:15
Mitochondria Structure and Funtion 1 Wallace Med Biochemistry 8/14/2007 8:00 8/14/2007 10:00
Mitochondria Structure and Funtion 2 Wallace Med Biochemistry 8/14/2007 10:15 8/14/2007 12:15
Lipids 1 Kaiser Med Biochemistry 8/15/2007 8:00 8/15/2007 10:00
Lipids 2 Kaiser Med Biochemistry 8/15/2007 10:15 8/15/2007 12:15
Membranes 1 Kaiser Carbohydrates 1 8/16/2007 8:00 8/16/2007 10:00
Membranes 2 Kaiser Med Biochemistry 8/16/2007 10:15 8/16/2007 12:15
Membranes 3 Kaiser Med Biochemistry 8/17/2007 8:00 8/17/2007 10:00
Protein Biochemistry 1 Steele Med Biochemistry 8/21/2007 8:00 8/21/2007 10:00
Protein Biochemistry 2 Steele Med Biochemistry 8/22/2007 8:00 8/22/2007 10:00
Protein Biochemistry 3 Steele Med Biochemistry 8/22/2007 10:15 8/22/2007 12:15
Protein Biochemistry 4 Steele Med Biochemistry 8/23/2007 8:00 8/23/2007 10:00
Amino Acid Metabolism 1 Dai Med Biochemistry 8/23/2007 10:15 8/23/2007 12:15
Amino Acid Metabolism 2 Dai Med Biochemistry 8/24/2007 8:00 8/24/2007 10:00
Donovan
18. Too much text can be distracting
The problem with putting too much text on the slide is that people feel
obligated to try and read what you have written. Consequently, they try
and read and then don’t pay full attention to what you are actually
saying. In other words it is distracting. Often that means that no-one
manages to actually finish reading what you have written before you are
ready to move on to the next slide. Then you move on to the next slide
and they are left sitting there thinking “Oh, I must have missed
something, maybe I should have listened instead of reading. But now it
is too late. Oh my god what am I going to do!”
But don’t worry there are solutions to this problem. Better to put bullets
of what you want to talk about and have the audience listen to you than
force them to have to read several entire paragraphs of writing in a small
font that they can hardly see from the back of the room.
Anyway, those are my thoughts on the matter. I don’t know what do you
think? You’re sitting there at the back of the room squinting and trying to
read all this crap. Do you have an opinion?
Donovan
19. Bullet statements
Problem
• Too many words on slide
• Audience is reading instead of listening
• Audience is confused and panicky!!!
Solution
• Use bullet statements
24. Lecture 1: Introduction to Stem Cell Biology
3/29/11
Introduction to the course
Presenting a research paper
Introduction to stem cell biology
Group discussion
25. Ancient Greeks
The first stem cell biologists?
According to Greek myth:
To punish Prometheus for giving fire back to the humans,
Zeus chained Prometheus to a boulder and had a
giant bird eat his liver.
At dusk each day, the bird flew away, Prometheus’ liver grew back,
the bird returned the following day to feast again.
26. The Dawn of the Nuclear Age
On August 6, 1945, the nuclear By the end of the year,
weapon, Little Boy, was dropped injury and radiation brought
on Hiroshima directly killing an total casualties to
estimated 70,000 people. 90,000-140,000 people.
29. Stem Cells Exist Throughout our Bodies
The human body has 10 trillion cells, more than 250 different types
Some tissues continually renew themselves from adult stem cells
Neuronal stem cell
Skin stem cell
Bone marrow stem cell Intestinal stem cell
30. Stem cells in modern medicine
• Stem cells in bone marrow can reconstitute blood cells
• First successful HPC transplants in 1960s
• Treatment for immunodeficiency disorders and leukemia
31. What are the key properties of stem cells?
1. Ability to self renew
2. Ability to develop into different types of mature cells
Nervous system
Heart
Muscle
Bone marrow
Pancreas
36. Multipotent Stem Cells
Able to form multiple cell types of one lineage
Hematopoietic
stem cells
Mesenchymal
stem cells
37. Unipotent Stem Cells
Able to form only one cell type.
Spermatogonial stem cells Sperm
38. Key Points
1. Key properties of stem cells
• Ability to self renew
• Ability to differentiate into a variety of specialized cells
2. Different types of stem cells
• Embryonic versus adult
• Tissue type
• Extent of ability to differentiate
– Totipotent
– Pluripotent
– Multipotent
– Unipotent
39. Lecture 1: Introduction to Stem Cell Biology
3/29/11
Introduction to the course
Presenting a research paper
Introduction to stem cell biology
Group discussion
40. Group Discussion
1. Name your group
2. Prepare a ppt slide
- group name
- group picture
- names of people in the group
3. Upload ppt to dropbox on class website
This greek myth may have been one of the first demonstrations of the existence of stem cells in adults. According to the story, P was punished for giving fire back to the humans by being chained to a rock and having a bird eat his liver each day. P was able to survive this punishment because the bird left at dusk and P liver grew back duriing the night. This myth points out the ability of the liver to regenerate a process that depends on the presence of liver stem cells.
The existence and importance of stem cells was also demonstrated in WWII. A nuclear bomb was dropped on Hiroshima. It immediately caused widespread destruction and the deaths of 70K people. Many additional deaths followed in the next months and year as a result of radiation exposure. Many of the deaths resulted from the radiation killing stem cells in the body. Normally these stem cells divide and differentiate to replace lost cells. They are present in organs such as the liver, gut, skin, etc.
This slide shows the function of stem cells in the skin. Normally,……
This slide shows the function of stem cells in the skin. High radiation kills diff cells progenitors and stem cells…so skin cannot be repaired…
A third well known example of the existence and importance of stem cells was first demonstrated in the 1960s. It was shown that bone marrow could be transplanted to treat certain blood disorders. This depended on the presence of blood stem cells in the bone marrow.
