Bio Sci D121          STEM CELL BIOLOGYLecture 1: Introduction to Stem Cell Biology                          4/3/10       ...
Instructor Information                  Leslie Lock, Ph.D.                  office: Gross Hall 3031                  e-mai...
Course InformationMeeting Time:      Tuesdays & Thursdays 9:30-10:45 am                   Regular attendance is expected a...
Course ObjectivesBy the end of this class you should be able to...Describe the sources, isolation, and characteristics of ...
Course RequirementsGrades will be based on the following:   Midterm exam                          30%         50% multiple...
Lecture Schedule
Lecture 1: Introduction to Stem Cell Biology                        3/29/11                Introduction to the course     ...
Week 2 Techniques Group Presentation
Week 4 Group Discussions
Week 9 Key Research Questions
Presenting your group’s research proposal           • Structure of talk           • Common problems in slides
Be Prepared•   Thoroughly understand information you are    presenting before the talk    – Ask someone• Practice the talk...
Giving a Presentation•   Start slowly to give the audience time to settle    down and focus on you•   Speak up!•   Talk to...
Frame Your Talk•   Tell people what you are going to tell them•   Tell it to them•   Tell them what you just told them    ...
Presenting your group’s research proposal           • Structure of talk           • Common problems in slides
Start and finish each slide• Have a title on each slide• Summarize the conclusions at the end of each slideDON’T USE ALL C...
Use a font that can be read from the back of the roomRemember, most of the faculty are old and cannot see well            ...
Too much text can be distractingThe problem with putting too much text on the slide is that people feelobligated to try an...
Bullet statementsProblem  • Too many words on slide  • Audience is reading instead of listening  • Audience is confused an...
Lack of labels can lead to confusion!                                        Donovan
Gene Expression in human ES Cells:            Microarray Analysis= overexpressed= underexpressed                          ...
Busy slides can be distracting                                 Donovan
Induced pluripotent stem cells:Induction, morphology and gene expression                B           C
Lecture 1: Introduction to Stem Cell Biology                         3/29/11                Introduction to the course    ...
Ancient Greeks    The first stem cell biologists?According to Greek myth:To punish Prometheus for giving fire back to the ...
The Dawn of the Nuclear AgeOn August 6, 1945, the nuclear     By the end of the year,weapon, Little Boy, was dropped    in...
The Remarkable Biology of Stem Cells              Stem Cell  The Niche
The Remarkable Biology of Stem Cells              Stem Cell  The Niche
Stem Cells Exist Throughout our Bodies The human body has 10 trillion cells, more than 250 different types Some tissues co...
Stem cells in modern medicine• Stem cells in bone marrow can reconstitute blood cells• First successful HPC transplants in...
What are the key properties of stem cells?  1. Ability to self renew  2. Ability to develop into different types of mature...
The Remarkable Biology of Stem Cells    Adult Stem CellPluripotent Stem Cell
Types of stem cellsOccurrence in development    • embryo    • adultLocation    • liver    • bone marrowVariety of speciali...
Totipotent cells         Able to form all lineages of the organismzygote            embryo        fetus                adult
Pluripotent Stem CellsAble to form all lineages in the body
Multipotent Stem CellsAble to form multiple cell types of one lineage    Hematopoietic      stem cells               Mesen...
Unipotent Stem Cells                   Able to form only one cell type.Spermatogonial stem cells                          ...
Key Points1.   Key properties of stem cells     •   Ability to self renew     •   Ability to differentiate into a variety ...
Lecture 1: Introduction to Stem Cell Biology                         3/29/11                Introduction to the course    ...
Group Discussion1. Name your group2. Prepare a ppt slide         - group name         - group picture         - names of p...
No tomorrow without stem cells
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  1. 1. Bio Sci D121 STEM CELL BIOLOGYLecture 1: Introduction to Stem Cell Biology 4/3/10 Introduction to the course Info about group presentations Introduction to stem cell biology Group discussion
  2. 2. Instructor Information Leslie Lock, Ph.D. office: Gross Hall 3031 e-mail: llock@uci.edu Peter Donovan, Ph.D. office: Gross Hall 3002 e-mail: pdonovan@uci.edu Alex Stover e-mail: astover@uci.edu Office HoursDr Lock: Wed 10:00 to 11am Gross Hall, 3031Dr Donovan: Fri 10:00 to 11am Gross Hall, 3002Alex Stover: Wed 1:00 to 2 pm BioSci III 2130 Class Website http://eee.uci.edu/12s/05370
  3. 3. Course InformationMeeting Time: Tuesdays & Thursdays 9:30-10:45 am Regular attendance is expected and advantageous, but not required.Meeting Place: Nat Sci II Rm 4201Prerequisites: BIOL 433 with grade of C or better.Format: Lecture / discussion. Group presentations & discussions of research papers.Text: None required.Recommended: Scott, CT, Stem Cell Now, Penguin Group, 2006 Goldstein, LSB & Schneider, M, Stem Cells for Dummies, 2010Reference texts: Lanza, R ed., Stem Cell Biology, 2nd ed. Elsevier Pub Co., 2009 Gilbert, S.F., Developmental Biology, 8th ed. Sinauer Pub Co., 2006Efforts will be made to accommodate special needs. It is the student’s responsibility tocommunicate the needs to DR. LOCK as soon as possible in order to make the necessaryarrangements.You are expected to uphold the universitys standards of academic honesty includingplagiarism on all exams.
  4. 4. Course ObjectivesBy the end of this class you should be able to...Describe the sources, isolation, and characteristics of stem cells.Compare and contrast totipotent, pluripotent, multipotent, and unipotent stem cells.Compare and contrast embryonic and tissue-specific (adult) stem cells.Evaluate uses of stem cells for studying and treating human disease.Interpret, evaluate, discuss, and present papers from the primary literature.
  5. 5. Course RequirementsGrades will be based on the following: Midterm exam 30% 50% multiple choice 50% short answer Final exam 40% 25% multiple choice 50% short answer 25% essay Group Discussions 10% Key Questions presentations 20%
  6. 6. Lecture Schedule
  7. 7. Lecture 1: Introduction to Stem Cell Biology 3/29/11 Introduction to the course Group discussions and presentations Introduction to stem cell biology Group discussion
  8. 8. Week 2 Techniques Group Presentation
  9. 9. Week 4 Group Discussions
  10. 10. Week 9 Key Research Questions
  11. 11. Presenting your group’s research proposal • Structure of talk • Common problems in slides
  12. 12. Be Prepared• Thoroughly understand information you are presenting before the talk – Ask someone• Practice the talk, then practice again∀ Χηεχκ ψουρ σλιδεσ βεφορε ψου ταλκ (Check your slides before your talk)• Know the equipment• Check video files Donovan
  13. 13. Giving a Presentation• Start slowly to give the audience time to settle down and focus on you• Speak up!• Talk to the audience not the screen• Convey your excitement about your proposal• Remember you are telling a story• Sometimes less is more Donovan
  14. 14. Frame Your Talk• Tell people what you are going to tell them• Tell it to them• Tell them what you just told them Donovan
  15. 15. Presenting your group’s research proposal • Structure of talk • Common problems in slides
  16. 16. Start and finish each slide• Have a title on each slide• Summarize the conclusions at the end of each slideDON’T USE ALL CAPITAL LETTERS • ALL CAPS IS DIFFICULT TO READ Donovan
  17. 17. Use a font that can be read from the back of the roomRemember, most of the faculty are old and cannot see well Thompson Med Biochemistry 8/8/2007 10:15 8/8/2007 12:15 Carbohydrates 2 Thompson Med Biochemistry 8/9/2007 10:15 8/9/2007 12:15 Carbohydrates 3 Thompson Med Biochemistry 8/10/2007 10:15 8/10/2007 12:15 Carbohydrates 4 Thompson Med Biochemistry 8/13/2007 9:00 8/13/2007 10:00 Carbohydrates 5 Thompson Med Biochemistry 8/13/2007 10:15 8/13/2007 12:15 Mitochondria Structure and Funtion 1 Wallace Med Biochemistry 8/14/2007 8:00 8/14/2007 10:00 Mitochondria Structure and Funtion 2 Wallace Med Biochemistry 8/14/2007 10:15 8/14/2007 12:15 Lipids 1 Kaiser Med Biochemistry 8/15/2007 8:00 8/15/2007 10:00 Lipids 2 Kaiser Med Biochemistry 8/15/2007 10:15 8/15/2007 12:15 Membranes 1 Kaiser Carbohydrates 1 8/16/2007 8:00 8/16/2007 10:00 Membranes 2 Kaiser Med Biochemistry 8/16/2007 10:15 8/16/2007 12:15 Membranes 3 Kaiser Med Biochemistry 8/17/2007 8:00 8/17/2007 10:00 Protein Biochemistry 1 Steele Med Biochemistry 8/21/2007 8:00 8/21/2007 10:00 Protein Biochemistry 2 Steele Med Biochemistry 8/22/2007 8:00 8/22/2007 10:00 Protein Biochemistry 3 Steele Med Biochemistry 8/22/2007 10:15 8/22/2007 12:15 Protein Biochemistry 4 Steele Med Biochemistry 8/23/2007 8:00 8/23/2007 10:00 Amino Acid Metabolism 1 Dai Med Biochemistry 8/23/2007 10:15 8/23/2007 12:15 Amino Acid Metabolism 2 Dai Med Biochemistry 8/24/2007 8:00 8/24/2007 10:00 Donovan
  18. 18. Too much text can be distractingThe problem with putting too much text on the slide is that people feelobligated to try and read what you have written. Consequently, they tryand read and then don’t pay full attention to what you are actuallysaying. In other words it is distracting. Often that means that no-onemanages to actually finish reading what you have written before you areready to move on to the next slide. Then you move on to the next slideand they are left sitting there thinking “Oh, I must have missedsomething, maybe I should have listened instead of reading. But now itis too late. Oh my god what am I going to do!”But don’t worry there are solutions to this problem. Better to put bulletsof what you want to talk about and have the audience listen to you thanforce them to have to read several entire paragraphs of writing in a smallfont that they can hardly see from the back of the room.Anyway, those are my thoughts on the matter. I don’t know what do youthink? You’re sitting there at the back of the room squinting and trying toread all this crap. Do you have an opinion? Donovan
  19. 19. Bullet statementsProblem • Too many words on slide • Audience is reading instead of listening • Audience is confused and panicky!!!Solution • Use bullet statements
  20. 20. Lack of labels can lead to confusion! Donovan
  21. 21. Gene Expression in human ES Cells: Microarray Analysis= overexpressed= underexpressed Donovan
  22. 22. Busy slides can be distracting Donovan
  23. 23. Induced pluripotent stem cells:Induction, morphology and gene expression B C
  24. 24. Lecture 1: Introduction to Stem Cell Biology 3/29/11 Introduction to the course Presenting a research paper Introduction to stem cell biology Group discussion
  25. 25. Ancient Greeks The first stem cell biologists?According to Greek myth:To punish Prometheus for giving fire back to the humans, Zeus chained Prometheus to a boulder and had a giant bird eat his liver.At dusk each day, the bird flew away, Prometheus’ liver grew back, the bird returned the following day to feast again.
  26. 26. The Dawn of the Nuclear AgeOn August 6, 1945, the nuclear By the end of the year,weapon, Little Boy, was dropped injury and radiation broughton Hiroshima directly killing an total casualties toestimated 70,000 people. 90,000-140,000 people.
  27. 27. The Remarkable Biology of Stem Cells Stem Cell The Niche
  28. 28. The Remarkable Biology of Stem Cells Stem Cell The Niche
  29. 29. Stem Cells Exist Throughout our Bodies The human body has 10 trillion cells, more than 250 different types Some tissues continually renew themselves from adult stem cells Neuronal stem cell Skin stem cellBone marrow stem cell Intestinal stem cell
  30. 30. Stem cells in modern medicine• Stem cells in bone marrow can reconstitute blood cells• First successful HPC transplants in 1960s• Treatment for immunodeficiency disorders and leukemia
  31. 31. What are the key properties of stem cells? 1. Ability to self renew 2. Ability to develop into different types of mature cells Nervous system Heart Muscle Bone marrow Pancreas
  32. 32. The Remarkable Biology of Stem Cells Adult Stem CellPluripotent Stem Cell
  33. 33. Types of stem cellsOccurrence in development • embryo • adultLocation • liver • bone marrowVariety of specialized cells formed • totipotent • pluripotent • multipotent • unipotent
  34. 34. Totipotent cells Able to form all lineages of the organismzygote embryo fetus adult
  35. 35. Pluripotent Stem CellsAble to form all lineages in the body
  36. 36. Multipotent Stem CellsAble to form multiple cell types of one lineage Hematopoietic stem cells Mesenchymal stem cells
  37. 37. Unipotent Stem Cells Able to form only one cell type.Spermatogonial stem cells Sperm
  38. 38. Key Points1. Key properties of stem cells • Ability to self renew • Ability to differentiate into a variety of specialized cells2. Different types of stem cells • Embryonic versus adult • Tissue type • Extent of ability to differentiate – Totipotent – Pluripotent – Multipotent – Unipotent
  39. 39. Lecture 1: Introduction to Stem Cell Biology 3/29/11 Introduction to the course Presenting a research paper Introduction to stem cell biology Group discussion
  40. 40. Group Discussion1. Name your group2. Prepare a ppt slide - group name - group picture - names of people in the group3. Upload ppt to dropbox on class website
  41. 41. No tomorrow without stem cells

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