Ques-1: What is the differential diagnosis of this patients infecting organism? Germ Tube Test is a screening diagnostic test. The differential diagnosis of this patient is obtain some urine samples for laboratory cultures in carbohydrate medium as \"pathogenic organism can reproduce by budding\" means it is \"pathogenic fungi\" infected at the surgical region of cervix. Therefore, it will produce germ tube with fungal spores upon cell cultures & culture will produce \"yellow precipitate\" when examined with I2-KI & NaOH (iodoform test) due to alcohol formation as a metabolic byproduct. The morphology of diagnostic samples with infected pathogen can be observed under the microscope. Ques-2. The organism was subsequently shown to form germ tubes. What is the organism? The organism was subsequently shown to form germ tubes depicting that the infected pathogen is “Candida albicans\", a fungal pathogen Ques-3. Is this organism part of the normal flora in humans? Yes, this species in humans mainly located normal commensual in the regions of mouth, gut and vagina. However, in the above surgical procedures of cervix, transition of the organism has occurred from commensalism to virulence by germ tube formation with hyperthermia (increased temperature). Solution Ques-1: What is the differential diagnosis of this patients infecting organism? Germ Tube Test is a screening diagnostic test. The differential diagnosis of this patient is obtain some urine samples for laboratory cultures in carbohydrate medium as \"pathogenic organism can reproduce by budding\" means it is \"pathogenic fungi\" infected at the surgical region of cervix. Therefore, it will produce germ tube with fungal spores upon cell cultures & culture will produce \"yellow precipitate\" when examined with I2-KI & NaOH (iodoform test) due to alcohol formation as a metabolic byproduct. The morphology of diagnostic samples with infected pathogen can be observed under the microscope. Ques-2. The organism was subsequently shown to form germ tubes. What is the organism? The organism was subsequently shown to form germ tubes depicting that the infected pathogen is “Candida albicans\", a fungal pathogen Ques-3. Is this organism part of the normal flora in humans? Yes, this species in humans mainly located normal commensual in the regions of mouth, gut and vagina. However, in the above surgical procedures of cervix, transition of the organism has occurred from commensalism to virulence by germ tube formation with hyperthermia (increased temperature)..