2. ABOUT THE PLANT
Cajanus cajan, also called Pigeon Pea plant
Short-term perennial shrub
Belongs to the Fabaceae family of the Dicot
group.
Its rich in proteins, carbohydrates and
minerals
Medicinal applications
3. IMPORTANT INFORMATION
Excellent source of anti-bacterial materials
Possible antibacterial component inside the
leaf: Cajanuslactone
4. STREPTOCOCCUS MUTANS
Inhibits the human oral cavity
Makes 36% of the total bacteria inhibiting
human teeth
Heterotrophic organism
Reproduce asexually
Facultative anaerobe
5. OBJECTIVE
Prove if Cajanus cajan contain anti-bacterial
properties against S.mutans and if filtrating or
not the extract will affect in any way the
antibacterial potential of the extract.
Hypotesis
The Cajanus cajan leaf extract will inhibit the
growth of S.mutans and the filtered extract will
have a better inhibitory effect than the unfiltered
extract.
6. MATHERIALS AND METHODS
Obtain extract by
Grind the leaves. adding water and Vacuum Carry out
processing it in a filtration of the aseptic
blender extract. techniques.
Make a bacterial Place the steril filter paper disc.
lawn with S. mutans Incubate the Petri dishes.
8. Table 1: Inhibition zone measurements
Measurements in mm
Trial 1 Trial 2 Trial 3
Cajanus cajan leaf extract 0.0 0.0 0.0
(unfiltered)
Cajanus cajan leaf extract 1.3 1.8 4.3
(filtered)
Control 0.0 0.9 0.0
9. Figure 1: Inhibition zone measurements
5
4.5
Size of inhibition zones (mm)
4
3.5
3
2.5 Leaf extract (unfiltered)
2 Leaf extract (filtered)
1.5 Control
1
0.5
0
Trial 1 Trial 2 Trial 3
Experimentation Trails
10. REFERENCES
BioWeb [Internet]. 2002. (WI):U.S.A. University of
Wisconsin System universities and centers. [cited Oct 15
2011]. Available in:
http://bioweb.uwlax.edu/bio203/s2007/allmann_ambe/
Ezeifeka, G.O., Orji, M.U., Mbata, T.I., Patrick, A.O. 2004.
Antimicrobial Activities of Cajanus cajan , Garcinia Kola
and Xylopia aethiopica on Patogenic Microorganism.
Biotechnology [Internet]. [cited 2011 Oct 15]; 3(1): 41-43.
Available in. Google Academics.
Kong, Y., Fu, Y., Zu, Y., Chang, F., Chen, Y., Liu, X.,
Stelten, J., Schiebel, H. 2010. Cajanuslactone, a new
coumarin with anti-bacterial activity from pigeon pea
[Cajanus cajan (L.) Millsp.] leaves. Food Chem [Internet].
[cited 2011 Oct 15]; 121(4): 1150-1155. Available in:
11. Raintree Nutrition [Internet]. 2005. Carson City (NV):
Raintree Nutrition, Inc. [updated 2010 Mar 20; cited 2011
Oct 15]. Available in: http://www.rain-
tree.com/guandu.htm
United States Department of Agriculture [Internet]. 2011.
Washington (D.C): Natural Resources Conservation
Services.[ 2011 Oct 15]. Available in:
http://plants.usda.gov/java/profile?symbol=CACA27
Wu, N., Kong, Y., Fu, Y., Zu, Y., Yang, Z., Yand, M., Peng,
X., Efferth, T. 2010. In Vitro Antioxidant Properties, DNA
Damage Protective Activity, and Xanthine Oxidase
Inhibitory Effect of Cajaninstilbene Acid, a Stilbene
Compound Derived from Pigeon Pea [Cajanus cajan (L.)
Millsp.] Leaves. J. Agric. Food Chem. [Internet]. [cited
2011 Oct 15]; 59(1): 437-443. Available in: ACS Chemistry
12. Zu, Y., Liu, X., Fu, Y., Wu, N., Kong, Y., Wink, M. 2010.
Chemical composition of the SFE-CO2 extracts from
Cajanus cajan (L.) Huth and their antimicrobial activity in
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