Complete the table
WHAT QUESTION DOES EACH ANSWER... WHAT IS THE GOAL FOR EACH...
Methods
Definition
Question that answers
Draw it
RNA seq
RNA seq or RNA sequencing is the examination of the RNA in a sample using next-generation
sequencing by analyzing the cellular transcriptome of RNA-encoded gene expression patterns.
MeDIP-Seq
MeDIP-Seq is an abbreviation that stands for Methylated DNA Immunoprecipitation
Sequencing. It is a technique used to examine patterns of DNA methylation in the genome.
Utilizing an antibody that binds specifically to methylated DNA, the DNA is then isolated and
sequenced. This enables researchers to identify methylated regions of the genome and investigate
their function. MeDIP-Seq can be used to answer queries such as how DNA methylation
influences gene expression, how it varies between cell types or tissues, and how it responds to
the environment.
HM450K
HM450K stands for HumanMethylation450 BeadChip by Illumina. It is a microarray-based
technique used to examine patterns of DNA methylation in the human genome. The device
contains probes that target over 450,000 CpG sites throughout the genome, enabling researchers
to identify methylated or unmethylated regions of the genome. HM450K can be used to answer
such queries as how DNA methylation patterns differ between healthy and diseased tissues, how
they change over time, and how they are influenced by the environment.
Mass Spec
The technique of mass spectrometry (MS) is used to analyze molecules based on their mass-to-
charge ratio. Utilized frequently in proteomics research to identify and quantify proteins in
biological samples. The method entails ionizing the sample molecules, separating them
according to their mass-to-charge ratio, and detecting them with a mass spectrometer. MS can be
used to determine which proteins are present in a biological sample, how much of each is
present, and how they interact.
EMSA
Electrophoretic Mobility Shift Assay stands for EMSA. It is a technique utilized to investigate
protein-DNA interactions. A labeled DNA probe is incubated with a protein extract from cells or
tissues, followed by electrophoresis on a polyacrylamide gel. If the protein binds to the DNA
probe, its electrophoretic mobility on the gel will change. EMSA can be used to determine which
proteins bind to a particular DNA sequence, as well as to investigate the kinetics and specificity
of protein-DNA interactions. This technique is frequently employed in the field of transcriptional
regulation, where it can be used to investigate how transcription factors bind to DNA and
regulate gene expression. Other protein-DNA interactions, such as those involved in DNA repair
or replication, can also be studied using EMSA. Overall, EMSA is an effective method for
investigating protein-DNA interactions and their function in cellular processes.
MNAse-Seq
MNase-Seq is an acronym that stands for Micrococcal Nuclease Sequencing. It is a technique
used to examine the structure and acce.
Complete the table WHAT QUESTION DOES EACH ANSWER... WHAT IS THE G.pdf
1. Complete the table
WHAT QUESTION DOES EACH ANSWER... WHAT IS THE GOAL FOR EACH...
Methods
Definition
Question that answers
Draw it
RNA seq
RNA seq or RNA sequencing is the examination of the RNA in a sample using next-generation
sequencing by analyzing the cellular transcriptome of RNA-encoded gene expression patterns.
MeDIP-Seq
MeDIP-Seq is an abbreviation that stands for Methylated DNA Immunoprecipitation
Sequencing. It is a technique used to examine patterns of DNA methylation in the genome.
Utilizing an antibody that binds specifically to methylated DNA, the DNA is then isolated and
sequenced. This enables researchers to identify methylated regions of the genome and investigate
their function. MeDIP-Seq can be used to answer queries such as how DNA methylation
influences gene expression, how it varies between cell types or tissues, and how it responds to
the environment.
HM450K
HM450K stands for HumanMethylation450 BeadChip by Illumina. It is a microarray-based
technique used to examine patterns of DNA methylation in the human genome. The device
contains probes that target over 450,000 CpG sites throughout the genome, enabling researchers
to identify methylated or unmethylated regions of the genome. HM450K can be used to answer
such queries as how DNA methylation patterns differ between healthy and diseased tissues, how
they change over time, and how they are influenced by the environment.
Mass Spec
2. The technique of mass spectrometry (MS) is used to analyze molecules based on their mass-to-
charge ratio. Utilized frequently in proteomics research to identify and quantify proteins in
biological samples. The method entails ionizing the sample molecules, separating them
according to their mass-to-charge ratio, and detecting them with a mass spectrometer. MS can be
used to determine which proteins are present in a biological sample, how much of each is
present, and how they interact.
EMSA
Electrophoretic Mobility Shift Assay stands for EMSA. It is a technique utilized to investigate
protein-DNA interactions. A labeled DNA probe is incubated with a protein extract from cells or
tissues, followed by electrophoresis on a polyacrylamide gel. If the protein binds to the DNA
probe, its electrophoretic mobility on the gel will change. EMSA can be used to determine which
proteins bind to a particular DNA sequence, as well as to investigate the kinetics and specificity
of protein-DNA interactions. This technique is frequently employed in the field of transcriptional
regulation, where it can be used to investigate how transcription factors bind to DNA and
regulate gene expression. Other protein-DNA interactions, such as those involved in DNA repair
or replication, can also be studied using EMSA. Overall, EMSA is an effective method for
investigating protein-DNA interactions and their function in cellular processes.
MNAse-Seq
MNase-Seq is an acronym that stands for Micrococcal Nuclease Sequencing. It is a technique
used to examine the structure and accessibility of chromatin in the genome. The technique
consists of digesting chromatin with micrococcal nuclease (MNase), an enzyme that cleaves
DNA in regions that are not firmly bound to histones. The fragments are then sequenced,
enabling researchers to identify regions of the genome that are more or less susceptible to MNase
digestion. MNase-Seq can be used to answer questions such as how chromatin structure differs
between cell types or tissues, how it alters during development or in response to the
environment, and how it influences gene expression.
3. FISH
FISH is an acronym that refers for Fluorescence in Situ Hybridization. It is a technique used to
visualize specific DNA or RNA sequences in cells or tissues. The technique entails hybridizing
probes that are fluorescently labeled and complementary to the target sequence, followed by
fluorescence microscopy imaging. FISH can be used to determine the location of specific genes
within the genome, how they are organized within the nucleus, and how they are expressed in
various cell types or tissues. FISH is a valuable diagnostic instrument because it can also detect
chromosomal abnormalities or gene fusions in cancer cells.
Methods
Definition
Question that answers
Draw it
RNA seq
RNA seq or RNA sequencing is the examination of the RNA in a sample using next-generation
sequencing by analyzing the cellular transcriptome of RNA-encoded gene expression patterns.
4. MeDIP-Seq
MeDIP-Seq is an abbreviation that stands for Methylated DNA Immunoprecipitation
Sequencing. It is a technique used to examine patterns of DNA methylation in the genome.
Utilizing an antibody that binds specifically to methylated DNA, the DNA is then isolated and
sequenced. This enables researchers to identify methylated regions of the genome and investigate
their function. MeDIP-Seq can be used to answer queries such as how DNA methylation
influences gene expression, how it varies between cell types or tissues, and how it responds to
the environment.
HM450K
HM450K stands for HumanMethylation450 BeadChip by Illumina. It is a microarray-based
technique used to examine patterns of DNA methylation in the human genome. The device
contains probes that target over 450,000 CpG sites throughout the genome, enabling researchers
to identify methylated or unmethylated regions of the genome. HM450K can be used to answer
such queries as how DNA methylation patterns differ between healthy and diseased tissues, how
they change over time, and how they are influenced by the environment.
Mass Spec
The technique of mass spectrometry (MS) is used to analyze molecules based on their mass-to-
charge ratio. Utilized frequently in proteomics research to identify and quantify proteins in
biological samples. The method entails ionizing the sample molecules, separating them
according to their mass-to-charge ratio, and detecting them with a mass spectrometer. MS can be
used to determine which proteins are present in a biological sample, how much of each is
present, and how they interact.
EMSA
Electrophoretic Mobility Shift Assay stands for EMSA. It is a technique utilized to investigate
protein-DNA interactions. A labeled DNA probe is incubated with a protein extract from cells or
tissues, followed by electrophoresis on a polyacrylamide gel. If the protein binds to the DNA
5. probe, its electrophoretic mobility on the gel will change. EMSA can be used to determine which
proteins bind to a particular DNA sequence, as well as to investigate the kinetics and specificity
of protein-DNA interactions. This technique is frequently employed in the field of transcriptional
regulation, where it can be used to investigate how transcription factors bind to DNA and
regulate gene expression. Other protein-DNA interactions, such as those involved in DNA repair
or replication, can also be studied using EMSA. Overall, EMSA is an effective method for
investigating protein-DNA interactions and their function in cellular processes.
MNAse-Seq
MNase-Seq is an acronym that stands for Micrococcal Nuclease Sequencing. It is a technique
used to examine the structure and accessibility of chromatin in the genome. The technique
consists of digesting chromatin with micrococcal nuclease (MNase), an enzyme that cleaves
DNA in regions that are not firmly bound to histones. The fragments are then sequenced,
enabling researchers to identify regions of the genome that are more or less susceptible to MNase
digestion. MNase-Seq can be used to answer questions such as how chromatin structure differs
between cell types or tissues, how it alters during development or in response to the
environment, and how it influences gene expression.
FISH
FISH is an acronym that refers for Fluorescence in Situ Hybridization. It is a technique used to
visualize specific DNA or RNA sequences in cells or tissues. The technique entails hybridizing
probes that are fluorescently labeled and complementary to the target sequence, followed by
fluorescence microscopy imaging. FISH can be used to determine the location of specific genes
within the genome, how they are organized within the nucleus, and how they are expressed in
various cell types or tissues. FISH is a valuable diagnostic instrument because it can also detect
chromosomal abnormalities or gene fusions in cancer cells.