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1 outline the steps involved in synthesizing AT and NADPH for use in the Calvin cycle. 2 Outline the 3 phases of the Calvin Cycle. Solution 1) STEPS INVOLVED IN SYNTHESIZING ATP AND NADPH Synthesis of ATP and NADPH occurs during the light-dependent reactions in the first phase of photosynthesis. The first phase of photosynthesis is to convert solar energy into chemical energy in the form of ATP and NADPH which will be used further in the light-independent reactions (Calvin\'s cycle). NADPH and ATP are produced from the combined work of protein complexes and pigment molecules. PRODUCTION OF ATP & NADPH This process takes place in a multiprotein complex called photosystem. The thylakoid membrane of the chloroplast consists of two photosystems. They are the, Photosystem I (P680) and Photosystem II (P700). PHOTOSYSTEMS These are the structural and functional units of protein complexes that are found in the thylakoid membranes of chloroplasts that carry out the absorption of light and trasfer of energy and electrons during photosynthesis. Each photosystem consists of multiple antenna proteins that contain a mixture of 300–400 chlorophyll a and b molecules, and also pigments like carotenoids. Cytochrome b6f complex and ATP synthase are the major protein complexes in the thylakoid membrane that work with the photosystems to create ATP and NADPH. STEPS INVOLVED Chlorophyll in the photosystem absorbs light energy. In PS II light energy is used to split water into 2 electrons, 2 hydrogen atoms and one oxygen molecule. Chlorophyll a in the reaction centre of PS II absorbs a photon which excites the electron in the molecule to a higher level. As the state of the eletron is highly unstable, the electron is transferred to another molecule creating a chain of reactions called Electron Transport Chain (ETC). Electrons flow from PS II to cytochrome b6f to PS I. During this transistion they loose energy and has to re energized. Hence another photon is absorbed by the protein antenna and the energy is transmitted to the PS I reaction centre known as P700 which gets oxidized and sends a high energy electron to reduce NADP+ to NADPH and releases oxygen as a waste product. ATP is produced by Cytochrome b6f and ATP Synthase by a process called photophospyrlation. Two types of photophosphorylation takes place during ATP synthesis. They are Cyclic photophosphorylation This process involves photosystem I and P700. Energy is provided to produce a proton gradient across the membrane that is used to power ATP synthase during chemiosmosis (movement of ions from higher to lower concentration) which helps in the conversion of ADP to ATP. Hence the electrons travel in a cylic manner back to the PS I, this reaction is called as cyclic photophosphoryltaion. Non-cyclic photophosphorylation Photosystem I and II are used in a series in non-cyclic photophosphorylation. P680 is the reaction centre invovled in this process and the electrons travel in a non-cyclic manner. Photolysis .
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Most life on Earth depends on photosynthesis.The process is carried out by plants, algae, and some types of bacteria, which capture energy from sunlight to produce oxygen (O2) and chemical energy stored in glucose (a sugar). Herbivores then obtain this energy by eating plants, and carnivores obtain it by eating herbivores. The process During photosynthesis, plants take in carbon dioxide (CO2) and water (H2O) from the air and soil. Within the plant cell, the water is oxidized, meaning it loses electrons, while the carbon dioxide is reduced, meaning it gains electrons. This transforms the water into oxygen and the carbon dioxide into glucose. The plant then releases the oxygen back into the air, and stores energy within the glucose molecules. Chlorophyll Inside the plant cell are small organelles called chloroplasts, which store the energy of sunlight. Within the thylakoid membranes of the chloroplast is a light-absorbing pigment called chlorophyll, which is responsible for giving the plant its green color. During photosynthesis, chlorophyll absorbs energy from blue- and red-light waves, and reflects green-light waves, making the plant appear green. Light-dependent reactions vs. light-independent reactions While there are many steps behind the process of photosynthesis, it can be broken down into two major stages: light-dependent reactions and light-independent reactions. The light-dependent reaction takes place within the thylakoid membrane and requires a steady stream of sunlight, hence the name light-dependent reaction. The chlorophyll absorbs energy from the light waves, which is converted into chemical energy in the form of the molecules ATP and NADPH. The light-independent stage, also known as the Calvin Cycle, takes place in the stroma, the space between the thylakoid membranes and the chloroplast membranes, and does not require light, hence the name light-independent reaction. During this stage, energy from the ATP and NADPH molecules is used to assemble carbohydrate molecules, like glucose, from carbon dioxide. C3 and C4 photosynthesis Not all forms of photosynthesis are created equal, however. There are different types of photosynthesis, including C3 photosynthesis and C4 photosynthesis. C3 photosynthesis is used by the majority of plants. It involves producing a three-carbon compound called 3-phosphoglyceric acid during the Calvin Cycle, which goes on to become glucose. C4 photosynthesis, on the other hand, produces a four-carbon intermediate compound, which splits into carbon dioxide and a three-carbon compound during the Calvin Cycle. A benefit of C4 photosynthesis is that by producing higher levels of carbon, it allows plants to thrive in environments without much light or water.
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Photosynthesis 2
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Photosynthesis Reactions
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