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SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2379
Morphometric Study of Pinna in Relation to Age in Uttar Pradesh
Population
Vidit Pratap Dixit
1
, Pratishtha Potdar
2*
, Jagmohan Singh Dhakar
3
1
Senior Demonstrator, Department of Anatomy, Rama Medical College Hospital and Research Centre, Hapur, U.P, India
2
Associate Professor, Department of Anatomy, Santosh Medical College, Ghaziabad, U.P, India
3
Statistician cum Assistant Professor, Department of Community Medicine, Santosh Medical College, Ghaziabad, U.P,
India
*Address for Correspondence: Dr. Pratishtha Potdar, Associate Professor, Department of Anatomy, Santosh Medical
College, Ghaziabad, India
E-mail: drpratishthagupta@gmail.com
Received: 11 Apr 2019/ Revised: 09 Jul 2019/ Accepted: 27 Aug 2019
ABSTRACT
Background: Morphometric dimensions of ear plays a very important role in plastic surgery and prosthetics. This study aimed to
determine different morphometric parameters of both ear and to provide information regarding age related changes.
Methods: A study was conducted on 167 subjects including both males and females. The ear was measured using vernier caliper
with an accuracy of 0.001 and recorded in millimeters (mm) in a data sheet. The size of pinna and the lobule were measured on
both right and left side and were correlated with age of the individual. Readings were statistically analyzed in order to determine
the relationship between the size of the pinna and the age of the individual.
Results: In our study of age 18–30 ear length was 6.15 cm, lobule length and width was 1.87 cm and 1.99 cm and in age 31–40 ear
length was 6.32 mm, lobular length and width was 1.95 cm and 2.01 cm and in age 41–50 yrs ear length was 6.415 cm, lobular
length and width was 1.98 cm and 2.06 cm. This shows that in our study ear length were increasing significantly with age and
similarly Rt and Lt lobular length and Lt lobular width were also increasing significantly with the age. There was no significant
difference between the size of the right and left pinna.
Conclusion: The present study shows that the expansion of auricle with age was related to structural change in auricular cartilage.
The ear morphometry also helps in predicting ear anomalies and to reproduce anatomically corrected ear during its
reconstruction.
Key-words: Ear auricle, Ear length, Ear lobule, Ear width, Morphopometry
INTRODUCTION
Human ear is a complex, curved inter wined substructure
as compared to rest of human body, its shape is framed
in such a way to allow spatial localization of sounds [1]
.
Human ear convey sign of age and gender that are ill
defined [2]
. The anatomical structures of the external ear
are utilized for personal identification of living subjects in
relation to criminal activity.
How to cite this article
Dixit V, Potdar P, Dhakar JS. Morphometric Study of Pinna in
Relation to Age in Uttar Pradesh Population. SSR Inst. Int. J. Life
Sci., 2019; 5(5): 2379-2386.
Access this article online
https://iijls.com/
There have been claims in recent years that the external
ear may be utilized for personal identification of both
living and deceased individuals [3]
. So knowledge about
normal auricular dimensions is important in diagnosis of
congenital malformation syndromes and acquired
deformities. The size of human auricle continues to
enlarge until advanced age and it is well known that this
structural changes of auricular cartilage is associated
with morphological age changes of elastic fibers, which
was one of important cause of expansion of auricle
even after adulthood [4,5]
. Many studies have been done
on expansion of ear auricle by measuring the size of
auricle but mechanism of expansion is still not clear [6]
.
Although various studies on morphological changes of
auricular cartilage with age changes and few on fine
structure of auricular cartilage have been reported but
Research Article
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2380
no literature are available on ultra structural age changes
in human auricular cartilage [7,8]
. The human ear is
divided into external, middle and internal parts. The
pinna and the external acoustic meatus form the
external ear. The lateral surface of the pinna is irregularly
concave, faces slightly forward and displays numerous
eminences and depressions (Fig. 1). It was of great
interest that elastic fibers in auricular cartilage
undergoes structural changes with age similar to dermis
of skin as this elastic fibers play a fine role in extension
of auricular length after puberty [9,10]
. In the present
study, we measured various parameters of human and
also determined the relationship between ear sizes with
age of the adult North Indian population.
Fig. 1: External features of ear
MATERIALS AND METHODS
The present study was carried out on the North Indian
population, in the age group of 18–60 years including
167 Males and 33 Females. Written consent was
obtained from each participant in a prescribed format.
Each participant was informed about the nature of the
study before obtaining the consent. Healthy individuals
in an age group of 18–60 years, without any deformity or
abnormality and willingness to participate in the study
were included in this study. Age groups below 18 and
above 65 years of age, having any type of physical
deformity or ear injury were excluded. The subjects were
divided into four groups according to their age at
intervals of 5 years.
The measurements related to total ear length and ear
width and lobule length and width were taken with a
digital Vernier Caliper as shown in Fig. 2.
Fig. 2: Digital Vernier Caliper
The observations were made according to the methods
of Eboh [11]
. Each subject was made to sit in a natural
head position in a chair with a backrest and positioned in
such a way so that the eyes of the subject looks straight
forward with the lower border of the eye sockets in the
same horizontal plane as the external auditory meatus.
Total ear length was measured as the distance from the
most superior point of the helix (A) to the most inferior
point of the ear lobule (B). Total ear width was
determined by measuring along the broadest part of the
pinna from the (D) to (C) as shown in Fig. 3.
Fig. 3: Left pinna showing the total length A to B and ear
width from C to D
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2381
Total lobule length was taken as the distance from the
tip of anti-tragus (E) to most inferior point of the lobule
(B) and the total lobule width was measured as the
horizontal distance of the lobule at the midpoint of the
lobule length (EB) as shown in Fig. 4.
Fig. 4: Left pinna lobule length E to B and lobule width
from F to G
Statistical Analysis- All the four parameters of right and
left ears were taken by digital venier caliper with an
accuracy of 0.001 and recorded in millimeters (mm) in a
data sheet. All subjects were divided into four groups
according to their age. The Pearson correlation is used to
establish the relationship between the size of the pinna
and the age of the individual and the data was analyzed
using SPSS version 23, p<0.05 was significant.
RESULTS
The pinna of 167 subjects were measured in the age
group of 18–57 years, which show that there was a
gradual increase in length of the pinna with advancing
age till 40–50 yrs and thereafter it is non-significant. The
results have been summarized as below.
Table 1 shows that there was a gradual increase in the
length of the pinna with advancing age till 40–50 yrs and
thereafter it was non-significant.
Table 1: Morphometric measurements of pinna in relation to age
Groups Age (Yrs)
Number of
subjects
Mean pinna length (mm) Mean ear width (mm)
REL LEL REW LEW
A 18-30 114 61.58±3.86 61.47±3.8 24.41±2.51 24.53±2.4
B 31-40 54 63.23±4.68 63.18±4.45 24.72±2.36 24.71±2.43
C 41-50 24 64.15±4.23 64.02±4.31 24.63±2.25 24.6±2.60
D 51-60 8 64.36±2.59 64.36±2.59 25.7±1.75 25.7±1.75
REL– Right ear length; LEL- Left ear length; REW- Right ear width; LEW- Left ear width
P-value (>0.005)= Non-significant
Table 2 shows that there was a gradual increase in length
and width of lobule from 18.79 and 19.57 mm at the age
of 18–30 yrs to 21.24 mm and 21.03 mm at the age of
51–60 with advancing age.
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2382
Fig. 5a: Relation between the lengths of pinna with age of individual
Series 1- Average length of right ear, Series 2- Average length left ear
Table 2: Morphometric measurements of lobule in relation to age
Groups Age (Yrs)
Number of
subjects
Mean lobule length (mm) Mean lobule width (mm)
RLL LLL RLW LLW
A 18-30 114 18.79±2.86 18.73±2.83 19.98±2.58 19.57±2.1
B 31-40 54 19.53±2.31 19.43±2.34 20.18±2.58 20.17±2.64
C 41-50 24 19.84±2.53 20.62±2.56 20.61±2.55 20.23±2.4
D 51-60 8 20.99±3.45 21.24±3.46 21.03±1.92 21.03±1.92
RLL-Right lobule length, LLL- LEFT lobule width, RLW- Right lobule width, LLW Left lobule width
Fig. 5 b: Relation between the lengths of lobule with age of individual
Series 1- Mean length of right lobule, Series 2- Mean length of left lobule
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2383
Table 3 shows that the right and left pinna length were
the same. Table 3 shows that right and left ear length
was 61.58 and 61.47 mm at the age of 18–30 yrs, 64.36
mm and 64.36 mm at the age of 51–60. This shows that
there was a non-significant difference between right and
left pinna length.
Table 3: Correlation between right and left pinna in relation to age
Groups Age (Yrs)
Number of
subjects
Mean pinna length (mm)
P- value
REL REL
A 18-30 114 61.58±3.86 61.47±3.80 0.82
B 31-40 54 63.23±4.68 63.18±4.45 0.95
C 41-50 24 64.15±4.23 64.02±4.31 0.91
D 51-60 8 64.36±2.59 64.36±2.59 1.00
P-value (>0.005)= Non-significant
Table 4 shows that right and left ear width was 24.41 and
24.53 mm at the age of 18–30 yrs, 25.7 mm and 25.7 mm
at the age of 51–60. This shows that there was non-
significant difference between right and left pinna width.
Table 4: Correlation of right and left pinna width in relation to age
Groups Age (Yrs)
Number of
subjects
Mean ear width (mm)
P-value
REW LEW
A 18-30 114 24.41±2.51 24.53±2.4 0.71
B 31-40 54 24.72±2.36 24.71±2.43 0.98
C 41-50 24 24.63±2.25 24.6±2.6 0.96
D 51-60 8 25.7±1.75 25.7±1.75 1.00
P-value (>0.005)= Non-significant
Table 5 shows that right and left lobular length was
18.79 and 18.73 mm at the age of 18–30 yrs, 20.99 mm
and 21.24 mm at the age of 51–60. This shows P-value
(>0.005), which means there was non-significant
difference between right and left lobular length.
Table 5: Correlation of right and left lobule length in relation to age
Groups Age (Yrs)
Number of
subjects
Mean lobule width (mm)
P- value
RLL LEL
A 18-30 114 18.79±2.86 18.73±2.83 0.83
B 31-40 54 19.53±2.31 19.43±2.34 0.82
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2384
C 41-50 24 19.84±2.53 20.62±2.56 0.29
D 51-60 8 20.99±3.45 21.24±3.46 0.88
P-value (>0.005)= Non-significant
Table 6 shows that right and left lobule width were 19.98
mm and 19.57 mm at the age of 18–30 yrs which was
increasing to 21.03 mm and 21.03 mm at the age of 51–
60 yrs, this shows that right and left lobular width have
the non-significant difference.
Table 6: Correlation of right and left lobule width in relation to age
Table 7 shows that the right ear length was increased
significantly with age, similarly right and left lobular
length and left lobular width were increasing significantly
with the age as p-value was <0.001.
Table 7: Correlation of ear dimensions with age
Total ear length (mm) Total ear width (mm)
Lobular length
(mm)
Lobular width (mm)
Right Left Right Left Right Left Right Left
Correlation
( r )
0.288 0.017 0.088 0.082 0.266 0.283 0.130 0.109
p-value 0.001 0.811 0.214 0.248 0.001 0.001 0.067 0.124
DISCUSSION
Morphometric data provides a valuable source of
information to ergonomists and designers, who
attempted to consider a range of body sizes and abilities
in the design of occupational environments and
products. Itoh et al. [12]
studied the morphological age
changes in adult human auricular cartilage in 1958
subjects and found that the histological changes of the
auricular cartilage containing elastic fibers may be one of
the reasons of ear length increases with age. Sharma et
al. [13]
in his morphometric study of ear lobule in 260
northwest Indians between the age of 1 to 80 years
observed that maximum length of the lobule increased
appreciably between 6–15 years and 41–80 years
similarly our study also shows that ear length and lobule
length increases from age 18 yrs to 60 yrs as 61.58 mm
on right ear and 61.47 on left ear at age of 18-30, which
was increased to 64.36 mm on right and 64.36 mm on
left ear at the age of 51–60 yrs.
Group Age (Yrs)
Number of
subjects
Mean lobule width in mm
P- value
Right Left
A 18-30 114 19.98±2.58 19.57±2.1 0.18
B 31-40 54 20.18±2.58 20.17±2.64 0.98
C 41-50 24 20.61±2.55 20.23±2.4 0.65
D 51-60 8 21.03±1.92 21.03±1.92 1.00
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2385
Liu [14]
incorporated anthropometric measurement on
200 hundred subjects with age 20-59 year, found that
pinna length was 52.8±8.7 mm in 20 year age group, in
30 year age group pinna length was 56.3±5.8 mm and 40
year age group pinna length was 56.6±6.90 mm, and in
50 year age group pinna length was 59.1±7.1 mm
showing increase in pinna length with age. Similarly in
our study pinna length was 61.58±3.86 mm at the age
18–30 yrs, 63.23±4.68 mm at the age 31–40 yrs,
64.15±4.32 at the age 41–50 yrs, 64.36±2.59 mm at the
age 51-60 yrs showing increase in pinna length with age.
Ekanem et al. [15]
did an anthropometric study of pinna
on 217 adult Nigerians in Maiduguri between 18 to 65
years and the subjects were divided into 11 groups
showed that the total ear length and total lobular length
are increasing significantly as same as our study.
Sullivan et al. [16]
shown in his morphometric study of the
external ear on 123 volunteers, 89 women and 34 men
with 18 to 65 years age. They divided all subjects into 3
groups with 15 year age intervals and measured total ear
length, lobule length and width. In a group of 15–30
years total ear length was 6.17 cm and lobule length and
width 1.78 cm and 2.10 cm similarly in our study of age
18–30 ear length was 6.15 cm, lobule length and width
was 1.87 cm and 1.99 cm. In 31–45 year age group, total
ear length was 6.27 cm, lobule length and width was
1.85, 1.96 cm respectively, similarly in our study of age
31–40 ear length was 6.32 mm, lobular length and width
was 1.95 cm and 2.01 cm. In 46–60 year age group total
ear length was 6.45 cm, lobule length and width was,
1.99 cm, 1.97 cm similarly in our study of age 41–50 yrs
ear length is 6.415 cm, lobular length and width was 1.98
cm was 2.06 cm this shows that ear length and lobular
length are increasing with age. Oludiran and Omotoso [17]
shown in the adult of 18 to 30 year, total ear length of
the left and the right side were 58.6±5.2 mm, 58.5±4.9
mm and width 33.3±4.8 mm, 34.0±4.9 mm. Total ear
lobular length of left and right side was15.8±3.3 mm,
15.9±3.6 mm and total ear lobule width 17.2±4.0 mm,
17.2±4.2 mm. There were no significant differences in
ear parameters of right and left side ear similar to our
study. Taura et al. [18]
reported that the correlation is
higher with height in right ear width, while in our study
ear length and lobular length and width were increased
significantly with the age. In our study we also explained
that increase in lobular length is not due to weight
effects of earing but due to ageing process. Deopa et al.
[19]
observed that there were differences in
anthropometric data of people from different regions in
India similarly Jung [20]
also surveyed the dimensions and
characteristic of Korean ears and found that age, gender
and different ethnic population were determinants of
ear dimensions. According to Kalra and Kalra [21]
, the
breadth of ear lobule increased up to the age of 15 yrs
nearest to 0–1 mm and almost static between 16–40 yrs
increases again from 41 yrs onwards while in our study
ear length and ear width were increasing significantly.
When we compare our study with those of others we
find there was a difference in the value of ear
measurement and these discrepancies could be result of
various factors such as race, genetic variable, individual
constitution, environment, age and human error.
CONCLUSIONS
In the present study, we confirmed that the auricular
size increased significantly after reaching adulthood until
advanced age by morphometric parameters of the ear. It
thus appeared that elastic fibers and components of the
extracellular matrix in the auricular cartilage changed
structurally with ageing. Therefore, it is concluded that
further study was required to provide a different
formula for different sex with large population size. With
these appropriate normative data, it is hoped that better
and objective reference material would be provided for
the aesthetic plastic surgeon, forensic purpose, in
particular, those engaged in ear rejuvenation in the
country.
CONTRIBUTION OF AUTHORS
Research concept- Dr. Pratishtha Potdar
Research design- Dr. Vidit Pratap Dixit
Supervision- Dr. Pratishtha Potdar
Materials- Dr. Vidit Pratap Dixit
Data collection- Dr. Vidit Pratap Dixit
Data analysis and Interpretation- Dr. Jagmohan Singh
Dhakar
Literature search- Dr. Jagmohan Singh Dhakar
Writing article- Dr. Pratishtha Potdar
Critical review- Dr. Jagmohan Singh Dhakar
Article editing- Dr. Pratishtha Potdar
Final approval- Dr. Pratishtha Potdar
REFERENCES
[1] Bozkar MG, Karakas P, Yavuz M, Dere F.
Morphometric of External Ear in our Adult
SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732
Dixit et al., 2019
DOI:10.21276/SSR-IIJLS.2019.5.5.2
Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2386
Population. Aesthetic Plast. Surg., 2006; 30(8): 81-85.
[2] Brucker JM, Patel J, Sullivan PK. A morphometric
study of the external ear: age- sex related difference.
Plast. Reconstr. Surg., 2003; 112(2): 647-53.
[3] Abbas A, Rutty GN. Ear piercing affects ear prints,
The role of ear piercing in human identification. J.
Forensic Sci., 2005; 50(2): 386-92.
[4] Ito I, Imada M, Ikeda M, Sueno K, Arikuni T, et al. A
morphological study of age changes in adult human
auricular cartilage with special emphasis on elastic
fibers. Larygoscope, 2001; 111: 881-86.
[5] Fox TF, Hon HK, Wong E, Chang A. Auricular
anthropometry of Hongkong Chinese babies. Clin.
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[6] Feathers DJ, Paquet V, Drury CG. Measurement
consistency and three-dimensional electro
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[7] Coward TJ, Scott B, Watson RM, Richard R. Laser
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[8] Kostovic-Knezevic L, Bradamante Z, Svajger A.
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[11]Eboh DEO. Morphological changes of the human
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[12]Itoh M, Ikeda, K Sueno, et al. Anthropometric study
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[13]Sharma A, Sidhu NK, Sharma MK, et al.
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[14]Liu BS. Incorporating anthropometry into design of
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[15]Ekanem AU, Garba SH, et al. Anthropometric study
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doi: 10.3923/jms.2010.176.180.
[16]Sullivan PK, Micheal J, Patel J. A Morphometric study
of the external ear: age and sex related differences.
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[17]Oludiran OO, Omotoso DR. A morphometric study of
the external ears at Benin City. Nig. J. Plast. Surg.,
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[18]Taura MG, Adamu LH, Gudaji A, Modibbo MH. High
Prediction from external ear morphometry; A pilot
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[19]Deopa D, Thakkar HK, Prakash C, Nirajan R, Barua
MP. Anthropometric measurement of external ear of
medical students in Uttarakhand Region. J.
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[20]Jung SH. Surveying the dimensions and
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[21] Kalra D, Kalra A. Anthrometric measurement of
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Open Access Policy:
Authors/Contributors are responsible for originality, contents, correct references, and ethical issues. SSR-IIJLS publishes all articles under Creative
Commons Attribution- Non-Commercial 4.0 International License (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/legalcode

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Morphometric_Study_Pinna_Relation_Age_Uttar_Pradesh_Population.pdf

  • 1. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2379 Morphometric Study of Pinna in Relation to Age in Uttar Pradesh Population Vidit Pratap Dixit 1 , Pratishtha Potdar 2* , Jagmohan Singh Dhakar 3 1 Senior Demonstrator, Department of Anatomy, Rama Medical College Hospital and Research Centre, Hapur, U.P, India 2 Associate Professor, Department of Anatomy, Santosh Medical College, Ghaziabad, U.P, India 3 Statistician cum Assistant Professor, Department of Community Medicine, Santosh Medical College, Ghaziabad, U.P, India *Address for Correspondence: Dr. Pratishtha Potdar, Associate Professor, Department of Anatomy, Santosh Medical College, Ghaziabad, India E-mail: drpratishthagupta@gmail.com Received: 11 Apr 2019/ Revised: 09 Jul 2019/ Accepted: 27 Aug 2019 ABSTRACT Background: Morphometric dimensions of ear plays a very important role in plastic surgery and prosthetics. This study aimed to determine different morphometric parameters of both ear and to provide information regarding age related changes. Methods: A study was conducted on 167 subjects including both males and females. The ear was measured using vernier caliper with an accuracy of 0.001 and recorded in millimeters (mm) in a data sheet. The size of pinna and the lobule were measured on both right and left side and were correlated with age of the individual. Readings were statistically analyzed in order to determine the relationship between the size of the pinna and the age of the individual. Results: In our study of age 18–30 ear length was 6.15 cm, lobule length and width was 1.87 cm and 1.99 cm and in age 31–40 ear length was 6.32 mm, lobular length and width was 1.95 cm and 2.01 cm and in age 41–50 yrs ear length was 6.415 cm, lobular length and width was 1.98 cm and 2.06 cm. This shows that in our study ear length were increasing significantly with age and similarly Rt and Lt lobular length and Lt lobular width were also increasing significantly with the age. There was no significant difference between the size of the right and left pinna. Conclusion: The present study shows that the expansion of auricle with age was related to structural change in auricular cartilage. The ear morphometry also helps in predicting ear anomalies and to reproduce anatomically corrected ear during its reconstruction. Key-words: Ear auricle, Ear length, Ear lobule, Ear width, Morphopometry INTRODUCTION Human ear is a complex, curved inter wined substructure as compared to rest of human body, its shape is framed in such a way to allow spatial localization of sounds [1] . Human ear convey sign of age and gender that are ill defined [2] . The anatomical structures of the external ear are utilized for personal identification of living subjects in relation to criminal activity. How to cite this article Dixit V, Potdar P, Dhakar JS. Morphometric Study of Pinna in Relation to Age in Uttar Pradesh Population. SSR Inst. Int. J. Life Sci., 2019; 5(5): 2379-2386. Access this article online https://iijls.com/ There have been claims in recent years that the external ear may be utilized for personal identification of both living and deceased individuals [3] . So knowledge about normal auricular dimensions is important in diagnosis of congenital malformation syndromes and acquired deformities. The size of human auricle continues to enlarge until advanced age and it is well known that this structural changes of auricular cartilage is associated with morphological age changes of elastic fibers, which was one of important cause of expansion of auricle even after adulthood [4,5] . Many studies have been done on expansion of ear auricle by measuring the size of auricle but mechanism of expansion is still not clear [6] . Although various studies on morphological changes of auricular cartilage with age changes and few on fine structure of auricular cartilage have been reported but Research Article
  • 2. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2380 no literature are available on ultra structural age changes in human auricular cartilage [7,8] . The human ear is divided into external, middle and internal parts. The pinna and the external acoustic meatus form the external ear. The lateral surface of the pinna is irregularly concave, faces slightly forward and displays numerous eminences and depressions (Fig. 1). It was of great interest that elastic fibers in auricular cartilage undergoes structural changes with age similar to dermis of skin as this elastic fibers play a fine role in extension of auricular length after puberty [9,10] . In the present study, we measured various parameters of human and also determined the relationship between ear sizes with age of the adult North Indian population. Fig. 1: External features of ear MATERIALS AND METHODS The present study was carried out on the North Indian population, in the age group of 18–60 years including 167 Males and 33 Females. Written consent was obtained from each participant in a prescribed format. Each participant was informed about the nature of the study before obtaining the consent. Healthy individuals in an age group of 18–60 years, without any deformity or abnormality and willingness to participate in the study were included in this study. Age groups below 18 and above 65 years of age, having any type of physical deformity or ear injury were excluded. The subjects were divided into four groups according to their age at intervals of 5 years. The measurements related to total ear length and ear width and lobule length and width were taken with a digital Vernier Caliper as shown in Fig. 2. Fig. 2: Digital Vernier Caliper The observations were made according to the methods of Eboh [11] . Each subject was made to sit in a natural head position in a chair with a backrest and positioned in such a way so that the eyes of the subject looks straight forward with the lower border of the eye sockets in the same horizontal plane as the external auditory meatus. Total ear length was measured as the distance from the most superior point of the helix (A) to the most inferior point of the ear lobule (B). Total ear width was determined by measuring along the broadest part of the pinna from the (D) to (C) as shown in Fig. 3. Fig. 3: Left pinna showing the total length A to B and ear width from C to D
  • 3. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2381 Total lobule length was taken as the distance from the tip of anti-tragus (E) to most inferior point of the lobule (B) and the total lobule width was measured as the horizontal distance of the lobule at the midpoint of the lobule length (EB) as shown in Fig. 4. Fig. 4: Left pinna lobule length E to B and lobule width from F to G Statistical Analysis- All the four parameters of right and left ears were taken by digital venier caliper with an accuracy of 0.001 and recorded in millimeters (mm) in a data sheet. All subjects were divided into four groups according to their age. The Pearson correlation is used to establish the relationship between the size of the pinna and the age of the individual and the data was analyzed using SPSS version 23, p<0.05 was significant. RESULTS The pinna of 167 subjects were measured in the age group of 18–57 years, which show that there was a gradual increase in length of the pinna with advancing age till 40–50 yrs and thereafter it is non-significant. The results have been summarized as below. Table 1 shows that there was a gradual increase in the length of the pinna with advancing age till 40–50 yrs and thereafter it was non-significant. Table 1: Morphometric measurements of pinna in relation to age Groups Age (Yrs) Number of subjects Mean pinna length (mm) Mean ear width (mm) REL LEL REW LEW A 18-30 114 61.58±3.86 61.47±3.8 24.41±2.51 24.53±2.4 B 31-40 54 63.23±4.68 63.18±4.45 24.72±2.36 24.71±2.43 C 41-50 24 64.15±4.23 64.02±4.31 24.63±2.25 24.6±2.60 D 51-60 8 64.36±2.59 64.36±2.59 25.7±1.75 25.7±1.75 REL– Right ear length; LEL- Left ear length; REW- Right ear width; LEW- Left ear width P-value (>0.005)= Non-significant Table 2 shows that there was a gradual increase in length and width of lobule from 18.79 and 19.57 mm at the age of 18–30 yrs to 21.24 mm and 21.03 mm at the age of 51–60 with advancing age.
  • 4. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2382 Fig. 5a: Relation between the lengths of pinna with age of individual Series 1- Average length of right ear, Series 2- Average length left ear Table 2: Morphometric measurements of lobule in relation to age Groups Age (Yrs) Number of subjects Mean lobule length (mm) Mean lobule width (mm) RLL LLL RLW LLW A 18-30 114 18.79±2.86 18.73±2.83 19.98±2.58 19.57±2.1 B 31-40 54 19.53±2.31 19.43±2.34 20.18±2.58 20.17±2.64 C 41-50 24 19.84±2.53 20.62±2.56 20.61±2.55 20.23±2.4 D 51-60 8 20.99±3.45 21.24±3.46 21.03±1.92 21.03±1.92 RLL-Right lobule length, LLL- LEFT lobule width, RLW- Right lobule width, LLW Left lobule width Fig. 5 b: Relation between the lengths of lobule with age of individual Series 1- Mean length of right lobule, Series 2- Mean length of left lobule
  • 5. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2383 Table 3 shows that the right and left pinna length were the same. Table 3 shows that right and left ear length was 61.58 and 61.47 mm at the age of 18–30 yrs, 64.36 mm and 64.36 mm at the age of 51–60. This shows that there was a non-significant difference between right and left pinna length. Table 3: Correlation between right and left pinna in relation to age Groups Age (Yrs) Number of subjects Mean pinna length (mm) P- value REL REL A 18-30 114 61.58±3.86 61.47±3.80 0.82 B 31-40 54 63.23±4.68 63.18±4.45 0.95 C 41-50 24 64.15±4.23 64.02±4.31 0.91 D 51-60 8 64.36±2.59 64.36±2.59 1.00 P-value (>0.005)= Non-significant Table 4 shows that right and left ear width was 24.41 and 24.53 mm at the age of 18–30 yrs, 25.7 mm and 25.7 mm at the age of 51–60. This shows that there was non- significant difference between right and left pinna width. Table 4: Correlation of right and left pinna width in relation to age Groups Age (Yrs) Number of subjects Mean ear width (mm) P-value REW LEW A 18-30 114 24.41±2.51 24.53±2.4 0.71 B 31-40 54 24.72±2.36 24.71±2.43 0.98 C 41-50 24 24.63±2.25 24.6±2.6 0.96 D 51-60 8 25.7±1.75 25.7±1.75 1.00 P-value (>0.005)= Non-significant Table 5 shows that right and left lobular length was 18.79 and 18.73 mm at the age of 18–30 yrs, 20.99 mm and 21.24 mm at the age of 51–60. This shows P-value (>0.005), which means there was non-significant difference between right and left lobular length. Table 5: Correlation of right and left lobule length in relation to age Groups Age (Yrs) Number of subjects Mean lobule width (mm) P- value RLL LEL A 18-30 114 18.79±2.86 18.73±2.83 0.83 B 31-40 54 19.53±2.31 19.43±2.34 0.82
  • 6. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2384 C 41-50 24 19.84±2.53 20.62±2.56 0.29 D 51-60 8 20.99±3.45 21.24±3.46 0.88 P-value (>0.005)= Non-significant Table 6 shows that right and left lobule width were 19.98 mm and 19.57 mm at the age of 18–30 yrs which was increasing to 21.03 mm and 21.03 mm at the age of 51– 60 yrs, this shows that right and left lobular width have the non-significant difference. Table 6: Correlation of right and left lobule width in relation to age Table 7 shows that the right ear length was increased significantly with age, similarly right and left lobular length and left lobular width were increasing significantly with the age as p-value was <0.001. Table 7: Correlation of ear dimensions with age Total ear length (mm) Total ear width (mm) Lobular length (mm) Lobular width (mm) Right Left Right Left Right Left Right Left Correlation ( r ) 0.288 0.017 0.088 0.082 0.266 0.283 0.130 0.109 p-value 0.001 0.811 0.214 0.248 0.001 0.001 0.067 0.124 DISCUSSION Morphometric data provides a valuable source of information to ergonomists and designers, who attempted to consider a range of body sizes and abilities in the design of occupational environments and products. Itoh et al. [12] studied the morphological age changes in adult human auricular cartilage in 1958 subjects and found that the histological changes of the auricular cartilage containing elastic fibers may be one of the reasons of ear length increases with age. Sharma et al. [13] in his morphometric study of ear lobule in 260 northwest Indians between the age of 1 to 80 years observed that maximum length of the lobule increased appreciably between 6–15 years and 41–80 years similarly our study also shows that ear length and lobule length increases from age 18 yrs to 60 yrs as 61.58 mm on right ear and 61.47 on left ear at age of 18-30, which was increased to 64.36 mm on right and 64.36 mm on left ear at the age of 51–60 yrs. Group Age (Yrs) Number of subjects Mean lobule width in mm P- value Right Left A 18-30 114 19.98±2.58 19.57±2.1 0.18 B 31-40 54 20.18±2.58 20.17±2.64 0.98 C 41-50 24 20.61±2.55 20.23±2.4 0.65 D 51-60 8 21.03±1.92 21.03±1.92 1.00
  • 7. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2385 Liu [14] incorporated anthropometric measurement on 200 hundred subjects with age 20-59 year, found that pinna length was 52.8±8.7 mm in 20 year age group, in 30 year age group pinna length was 56.3±5.8 mm and 40 year age group pinna length was 56.6±6.90 mm, and in 50 year age group pinna length was 59.1±7.1 mm showing increase in pinna length with age. Similarly in our study pinna length was 61.58±3.86 mm at the age 18–30 yrs, 63.23±4.68 mm at the age 31–40 yrs, 64.15±4.32 at the age 41–50 yrs, 64.36±2.59 mm at the age 51-60 yrs showing increase in pinna length with age. Ekanem et al. [15] did an anthropometric study of pinna on 217 adult Nigerians in Maiduguri between 18 to 65 years and the subjects were divided into 11 groups showed that the total ear length and total lobular length are increasing significantly as same as our study. Sullivan et al. [16] shown in his morphometric study of the external ear on 123 volunteers, 89 women and 34 men with 18 to 65 years age. They divided all subjects into 3 groups with 15 year age intervals and measured total ear length, lobule length and width. In a group of 15–30 years total ear length was 6.17 cm and lobule length and width 1.78 cm and 2.10 cm similarly in our study of age 18–30 ear length was 6.15 cm, lobule length and width was 1.87 cm and 1.99 cm. In 31–45 year age group, total ear length was 6.27 cm, lobule length and width was 1.85, 1.96 cm respectively, similarly in our study of age 31–40 ear length was 6.32 mm, lobular length and width was 1.95 cm and 2.01 cm. In 46–60 year age group total ear length was 6.45 cm, lobule length and width was, 1.99 cm, 1.97 cm similarly in our study of age 41–50 yrs ear length is 6.415 cm, lobular length and width was 1.98 cm was 2.06 cm this shows that ear length and lobular length are increasing with age. Oludiran and Omotoso [17] shown in the adult of 18 to 30 year, total ear length of the left and the right side were 58.6±5.2 mm, 58.5±4.9 mm and width 33.3±4.8 mm, 34.0±4.9 mm. Total ear lobular length of left and right side was15.8±3.3 mm, 15.9±3.6 mm and total ear lobule width 17.2±4.0 mm, 17.2±4.2 mm. There were no significant differences in ear parameters of right and left side ear similar to our study. Taura et al. [18] reported that the correlation is higher with height in right ear width, while in our study ear length and lobular length and width were increased significantly with the age. In our study we also explained that increase in lobular length is not due to weight effects of earing but due to ageing process. Deopa et al. [19] observed that there were differences in anthropometric data of people from different regions in India similarly Jung [20] also surveyed the dimensions and characteristic of Korean ears and found that age, gender and different ethnic population were determinants of ear dimensions. According to Kalra and Kalra [21] , the breadth of ear lobule increased up to the age of 15 yrs nearest to 0–1 mm and almost static between 16–40 yrs increases again from 41 yrs onwards while in our study ear length and ear width were increasing significantly. When we compare our study with those of others we find there was a difference in the value of ear measurement and these discrepancies could be result of various factors such as race, genetic variable, individual constitution, environment, age and human error. CONCLUSIONS In the present study, we confirmed that the auricular size increased significantly after reaching adulthood until advanced age by morphometric parameters of the ear. It thus appeared that elastic fibers and components of the extracellular matrix in the auricular cartilage changed structurally with ageing. Therefore, it is concluded that further study was required to provide a different formula for different sex with large population size. With these appropriate normative data, it is hoped that better and objective reference material would be provided for the aesthetic plastic surgeon, forensic purpose, in particular, those engaged in ear rejuvenation in the country. CONTRIBUTION OF AUTHORS Research concept- Dr. Pratishtha Potdar Research design- Dr. Vidit Pratap Dixit Supervision- Dr. Pratishtha Potdar Materials- Dr. Vidit Pratap Dixit Data collection- Dr. Vidit Pratap Dixit Data analysis and Interpretation- Dr. Jagmohan Singh Dhakar Literature search- Dr. Jagmohan Singh Dhakar Writing article- Dr. Pratishtha Potdar Critical review- Dr. Jagmohan Singh Dhakar Article editing- Dr. Pratishtha Potdar Final approval- Dr. Pratishtha Potdar REFERENCES [1] Bozkar MG, Karakas P, Yavuz M, Dere F. Morphometric of External Ear in our Adult
  • 8. SSR Inst. Int. J. Life Sci. ISSN (O): 2581-8740 | ISSN (P): 2581-8732 Dixit et al., 2019 DOI:10.21276/SSR-IIJLS.2019.5.5.2 Copyright © 2015 - 2019| SSR-IIJLS by Society for Scientific Research under a CC BY-NC 4.0 International License Volume 05 | Issue 05 | Page 2386 Population. Aesthetic Plast. Surg., 2006; 30(8): 81-85. [2] Brucker JM, Patel J, Sullivan PK. A morphometric study of the external ear: age- sex related difference. Plast. Reconstr. Surg., 2003; 112(2): 647-53. [3] Abbas A, Rutty GN. Ear piercing affects ear prints, The role of ear piercing in human identification. J. Forensic Sci., 2005; 50(2): 386-92. [4] Ito I, Imada M, Ikeda M, Sueno K, Arikuni T, et al. A morphological study of age changes in adult human auricular cartilage with special emphasis on elastic fibers. Larygoscope, 2001; 111: 881-86. [5] Fox TF, Hon HK, Wong E, Chang A. Auricular anthropometry of Hongkong Chinese babies. Clin. Ortho. Dont. Res., 2004; 7: 10-14. [6] Feathers DJ, Paquet V, Drury CG. Measurement consistency and three-dimensional electro mechanical anthropometry. Int. J. Industrial Ergonomics, 2004; 33: 181-90. [7] Coward TJ, Scott B, Watson RM, Richard R. Laser scanning of ear identifying the shape and position in subjects with normal facial symmetry. Int. J. Oral Maxillofac. Surg., 2000; 29: 18-23. [8] Kostovic-Knezevic L, Bradamante Z, Svajger A. Ultrastructure of elastic cartilage in rat external ear. Cell Tissue Res., 2001; 218(1): 149-60. [9] De carvalho Filho ET, De Souzo RR, et al. Age related changes in elastic fibers of human heart. Gerentology, 2003; 42; 211-17. [10]Brucker MJ, Patel J, Sullivan PK. A morphometric study of the external ear; Age and sex related differences. Plast. Reconstr. Surg., 2003; 112(2); 647- 52. [11]Eboh DEO. Morphological changes of the human pinna in relation to age and gender of Urhobo people in Southern Nigeria. J. Exp. Clin. Anat., 2013; 12: 68- 74. [12]Itoh M, Ikeda, K Sueno, et al. Anthropometric study on normal human auricle in Japan. 2001; 104(2): 165-74. doi: 10.3950/jibiinkoka.104.165. [13]Sharma A, Sidhu NK, Sharma MK, et al. Morphometric study of ear lobule in northwest Indian male subjects. Anat. Sci. Int., 2007; 82(2): 98- 104. doi: 10.1111/j.1447-073X.2007.00166.x. [14]Liu BS. Incorporating anthropometry into design of ear-related products. Appl. Ergon. 2008; 39(1):115- 21. [15]Ekanem AU, Garba SH, et al. Anthropometric study of the pinna (auricle) among adult Nigerian resident in Maiduguri metropolis. J. Med. Sci., 2010; 176-80. doi: 10.3923/jms.2010.176.180. [16]Sullivan PK, Micheal J, Patel J. A Morphometric study of the external ear: age and sex related differences. J. Plastic Surg., 2010; 401-06. [17]Oludiran OO, Omotoso DR. A morphometric study of the external ears at Benin City. Nig. J. Plast. Surg., 2012; 8(1): 1-5. [18]Taura MG, Adamu LH, Gudaji A, Modibbo MH. High Prediction from external ear morphometry; A pilot study. Int. J. Res. Heal. Sci., 2016; 4(1): 15-19. [19]Deopa D, Thakkar HK, Prakash C, Nirajan R, Barua MP. Anthropometric measurement of external ear of medical students in Uttarakhand Region. J. Anatomical Society India, 62, 2013; 79-83. [20]Jung SH. Surveying the dimensions and characteristics of Korean ears for the ergonomics design of ear-related products. Int. J. Ind. Ergon., 2003; 31: 361-73. [21] Kalra D, Kalra A. Anthrometric measurement of external ear. Int. J. Enhanced Res. Med. Dental care, 2015; 2(3): 10-16. Open Access Policy: Authors/Contributors are responsible for originality, contents, correct references, and ethical issues. SSR-IIJLS publishes all articles under Creative Commons Attribution- Non-Commercial 4.0 International License (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/legalcode