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Order the steps to make monoclonal antibodies- Remove unfused cells- E.pdf

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lonkarhrishikesh

Order the steps to make monoclonal antibodies. Remove unfused cells. Extract spleen cells. Grow each hybridoma on its own plate. Inject lab mice with the antigen of interest. Screen cell colonies to find the antibody of interest. Force mouse B-cells and myeloma cells together. Match the genetic engineering term to its description. Specialized plasmids or other genetic elements that will hold any piece of foreign DNA A short segment of DNA which contains many unique restriction sites close together Nucleotide sequences from two different sources are combined in vitro into the same DNA molecule Short nucleotide sequence added to the end of a DNA insert to create a sticky end A virus that uses bacteria as a host Inserting target DNA into a fragment of the LacZa gene.

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Order the steps to make monoclonal antibodies. Remove unfused cells. Extract spleen cells. Grow each hybridoma on its own plate. Inject lab mice with the antigen of interest. Screen cell colonies to find the antibody of interest. Force mouse B-cells and myeloma cells together. Match the genetic engineering term to its description. Specialized plasmids or other genetic elements that will hold any piece of foreign DNA A short segment of DNA which contains many unique restriction sites close together Nucleotide sequences from two different sources are combined in vitro into the same DNA molecule Short nucleotide sequence added to the end of a DNA insert to create a sticky end A virus that uses bacteria as a host Inserting target DNA into a fragment of the LacZa gene

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