Order the steps to make monoclonal antibodies- Remove unfused cells- E.pdf
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Order the steps to make monoclonal antibodies. Remove unfused cells. Extract spleen cells. Grow each hybridoma on its own plate. Inject lab mice with the antigen of interest. Screen cell colonies to find the antibody of interest. Force mouse B-cells and myeloma cells together. Match the genetic engineering term to its description. Specialized plasmids or other genetic elements that will hold any piece of foreign DNA A short segment of DNA which contains many unique restriction sites close together Nucleotide sequences from two different sources are combined in vitro into the same DNA molecule Short nucleotide sequence added to the end of a DNA insert to create a sticky end A virus that uses bacteria as a host Inserting target DNA into a fragment of the LacZa gene.
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[22, 29, 50, 36, 12, 27, 25, 26, 23, 37, 45, 20, 14, 43, 48, 9, 18, 44, 20, 21, 50, 44, 6, 16], [50, 7,
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48, 26, 36, 38, 11, 15], [37, 21, 7, 22, 23, 43, 29, 24, 42, 16, 45, 16, 43, 5, 37, 12, 46, 9, 44, 22,
19, 34, 39, 13], [50, 39, 8, 48, 23, 34, 13, 46, 19, 42, 20, 17, 31, 34, 10, 22, 42, 48, 32, 21, 29, 47,
21, 44], [26, 32, 34, 24, 31, 32, 32, 36, 42, 22, 48, 8, 19, 38, 17, 36, 41, 14, 34, 44, 30, 19, 30,
50], [17, 28, 45, 49, 34, 21, 48, 38, 43, 31, 37, 21, 40, 22, 16, 39, 37, 29, 46, 25, 35, 27, 27, 49],
[11, 7, 45, 18, 49, 8, 11, 37, 36, 38, 32, 10, 41, 34, 32, 24, 23, 18, 43, 47, 16, 6, 28, 32], [42, 6,
42, 47, 30, 15, 5, 33, 17, 20, 5, 47, 20, 27, 22, 43, 14, 39, 6, 45, 21, 48, 27, 22], [47, 20, 24, 18,
21, 6, 34, 30, 33, 9, 43, 21, 23, 10, 35, 23, 35, 33, 29, 16, 38, 45, 40, 38], [31, 6, 8, 11, 36, 8, 25,
24, 47, 7, 30, 14, 22, 32, 45, 22, 21, 12, 16, 30, 17, 21, 36, 24], [40, 32, 10, 26, 18, 17, 35, 11, 47,
20, 36, 7, 16, 37, 30, 8, 40, 41, 13, 45, 13, 30, 29, 14], [22, 35, 42, 6, 26, 11, 7, 42, 6, 16, 6, 21,
32, 38, 21, 37, 39, 30, 13, 43, 49, 29, 21, 45], [33, 46, 44, 11, 15, 21, 34, 8, 46, 50, 33, 34, 9, 16,
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vessel walls Platelet aggregation and formation of fibrin network Activation of stem cells and
remodelling (cell growth, movement, death and phagocytosis) Recruitment of immune cells and
chondrocytes (collagen producing cells).
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ou have been assigned to a team that will be developing a system dinal Exam formation System
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diam. You have been your project Student. ID. mambers. A Member can be a Proft. A
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Draw a class diagram, showing the classes, their attributes, operations, and rel.
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ou are a hospital administrator responsible for staffing the ER
The following is a list of the number of people in the ER waiting room each hour
over a 24 hour period (i.e the first measure is at 12am, the second at 1am etc)
for 28 days starting on a Sunday (i.e the first list covers the 24 hours of Sunday,
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The hospital staffs on 3 shifts:
Shift #1: 12am - 8am
Shift #2: 8am - 4pm
Shift #3: 4pm - 12am
In this assignment you are to determine and output the following for each shift on each day of the
week:
average number of people in the waiting room
highest number of people in the waiting room
lowest number of people in the waiting room
range of the number of people in the waiting room
NB: You will output 82 values (7 days * 3 shifts * 4 measures). Your output should be
appropriated titled ie by shift number and day of the week.
The data you are to use in your analysis is:
data = [[41, 12, 43, 25, 39, 16, 13, 20, 10, 22, 34, 44, 40, 12, 14, 20, 10, 21, 28, 44, 5, 30, 7, 18],
[22, 29, 50, 36, 12, 27, 25, 26, 23, 37, 45, 20, 14, 43, 48, 9, 18, 44, 20, 21, 50, 44, 6, 16], [50, 7,
37, 36, 5, 12, 23, 15, 13, 9, 33, 21, 22, 24, 45, 29, 44, 31, 44, 21, 29, 33, 31, 21], [38, 14, 7, 50,
14, 31, 32, 32, 41, 7, 50, 50, 32, 18, 19, 26, 17, 48, 20, 41, 40, 47, 33, 27], [30, 47, 17, 34, 35, 29,
20, 29, 41, 45, 23, 18, 10, 35, 17, 26, 19, 15, 15, 35, 6, 24, 48, 34], [11, 38, 48, 8, 44, 14, 19, 46,
50, 21, 31, 48, 28, 38, 33, 41, 26, 43, 50, 34, 20, 8, 20, 5], [43, 34, 22, 20, 37, 29, 40, 28, 17, 32,
32, 7, 29, 18, 31, 6, 22, 14, 9, 33, 5, 36, 40, 16], [15, 13, 46, 36, 35, 8, 23, 15, 27, 35, 17, 33, 40,
26, 45, 9, 32, 37, 25, 26, 41, 47, 27, 22], [43, 35, 38, 34, 21, 18, 13, 6, 6, 47, 13, 41, 43, 46, 40, 5,
17, 10, 10, 10, 36, 19, 24, 12], [38, 43, 40, 12, 45, 17, 34, 26, 16, 30, 12, 46, 33, 37, 5, 18, 48, 29,
48, 26, 36, 38, 11, 15], [37, 21, 7, 22, 23, 43, 29, 24, 42, 16, 45, 16, 43, 5, 37, 12, 46, 9, 44, 22,
19, 34, 39, 13], [50, 39, 8, 48, 23, 34, 13, 46, 19, 42, 20, 17, 31, 34, 10, 22, 42, 48, 32, 21, 29, 47,
21, 44], [26, 32, 34, 24, 31, 32, 32, 36, 42, 22, 48, 8, 19, 38, 17, 36, 41, 14, 34, 44, 30, 19, 30,
50], [17, 28, 45, 49, 34, 21, 48, 38, 43, 31, 37, 21, 40, 22, 16, 39, 37, 29, 46, 25, 35, 27, 27, 49],
[11, 7, 45, 18, 49, 8, 11, 37, 36, 38, 32, 10, 41, 34, 32, 24, 23, 18, 43, 47, 16, 6, 28, 32], [42, 6,
42, 47, 30, 15, 5, 33, 17, 20, 5, 47, 20, 27, 22, 43, 14, 39, 6, 45, 21, 48, 27, 22], [47, 20, 24, 18,
21, 6, 34, 30, 33, 9, 43, 21, 23, 10, 35, 23, 35, 33, 29, 16, 38, 45, 40, 38], [31, 6, 8, 11, 36, 8, 25,
24, 47, 7, 30, 14, 22, 32, 45, 22, 21, 12, 16, 30, 17, 21, 36, 24], [40, 32, 10, 26, 18, 17, 35, 11, 47,
20, 36, 7, 16, 37, 30, 8, 40, 41, 13, 45, 13, 30, 29, 14], [22, 35, 42, 6, 26, 11, 7, 42, 6, 16, 6, 21,
32, 38, 21, 37, 39, 30, 13, 43, 49, 29, 21, 45], [33, 46, 44, 11, 15, 21, 34, 8, 46, 50, 33, 34, 9, 16,
42, 50, 31, 19, 29, 14, 17, 27, 42, 10], [11, 40, 24, 47, 8, 37, 21, 12, 21, 25, 11, 37, 24, 22, 37, .
Otvidends per share Sandpiper Company has 10-000 shares of cumulative.pdf
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Options- 1) undercounting double crossover events 2) each two point di.pdf
Options:
1) undercounting double crossover events
2) each two point distance is estimated from independent cross
3) both 1 and 2
4) none of the above.
Please answer and explain.
What is/are likely contributors to the issue that internal distances from a set of 2 pt crosses do not
often sum to the distance estimated directly for the pair of outer genes?.
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