This is a folging of two news about DNA replication. i hope you like it

1. DNA REPLICATION
Laura Restrepo Carvajal
Molecular Biology
Teacher: Lina Martínez
Medicine
UPB-Medellin
August 13/2012

4. In the replication one strand is synthesized
continuously and is called the leading strand,
the other is synthesizes discontinue from small
fragments called Okasaki fragments and RNA
using primers
the origin of replication site is a short repeated
sequences that is recognized by proteins
binding to sites of origin
DNA polymerases synthesize DNA only in
the direction 5 to 3, adding a dNTP to the
group 3 hydroxyl a emerging chain

5. Protein involved in DNA replication centrosome
regulation liked to Dwafism and small brain size

6. Protein involved in DNA replication centrosome
regulation linked to Dwafism and small brain size
Other roles for ORC, includes centrosome
regulation and in that secuence , ORC can be
a regulator of the cromosomic division , cause
centrosome ensures that the duplicated
chromosomes are segregated evenly to the
daughter cells.

7. Protein involved in DNA replication centrosome
regulation linked to Dwafism and small brain size
MGS patients often have
mutations in the gene encoding
the ORC therefore does not
prevent the centrosome
duplication several times.

8. Protein involved in DNA replication centrosome
regulation linked to Dwafism and small brain size
The centrosome also plays
a role in establishing cell
outgrowths, such as axons
in brain cells and cilia in
many other cells of the
body.

9. Observation
Early detection of mutation of genes encoding the ORC
could give more possibilities of treatment to people
suffering from the MGS.

10. New England Biolabs develop novel polymerase
with ultra –high fidelity and minimal GS bias

11. New England Biolabs develop novel polymerase
with ultra –high fidelity and minimal GS bias
High fidelity amplification is
essential for experiments whose
outcome depends upon the
correct DNA sequence, such as
sequencing, SNP detection,
protein expression and gene
function studies.

12. New England Biolabs develop novel polymerase
with ultra –high fidelity and minimal GS bias
Q5 High-Fidelity DNA Polymerase sets a new standard for both
fidelity and robust performance. With the highest fidelity
amplification available (>50 times higher than Taq) that is frequently
used in polymerase chain reaction (PCR)

13. New England Biolabs develop novel polymerase
with ultra –high fidelity and minimal GS bias
A hot start version of the
Q5 polymerase, which
utilizes a synthetic aptamer
for room-temperature set
up, is also available.

14. Observation
the uniform amplification of sequences, with robustness, coverage, high
speed , and a great amplicon length allows the efficiency of the PCR
technique

15. Medical Utility

16. Medical Utility
Survival of an organism depends
on the accurate replication of its
genome, an error in the synthesis
generates a permanent mutation
and the possible elimination of
the cell line

17. Medical Utility
This research has significant implications
for processes that control body and brain
size; how tissues come to be the size that
they are is a little understood area of
biology.
Studying how mutations in centrosome
biology affect tissue and body size may
lead to insights into this regulation,
Stillman suggests

18. Medical Utility
These new types of PCR award according to
their characteristics, greater certainty to the
diagnosis of malignant diseases such
as leukemia and lymphomas ,as will be made
much easier and faster identification of virus
or bacteria causing a disease like SIDA,
identify persons (bodies) or do scientific
research on DNA amplified

19. Bibliography
• MARTINEZ SANCHEZ, Lina María. Biología molecular.7.ed.Medellín: UPB.Fac.de
Medicina,2012.74-84p
• New England Biolabs Inc. (2012, 07). neb.com. Retrieved 08 09, 2012, from
http://www.neb.com:
http://www.neb.com/nebecomm/tech_reference/polymerases/Q5.asp#.UCbY0Z3v-
TI
• Biosciencetechnology. (2012, 08 1). Retrieved 08 2, 2012, from
biosciencetechnology.com: www.biosciencetechnology.com/News/2012/08/Protein-
Involved-in-DNA-Replication,-Centrosome-Regulation-Linked-to-Dwarfism,-Small-
Brain-Size/

20. “LIFE IS WHAT HAPPENS TO
YOU WHILE YOU'RE BUSY
MAKING OTHER PLANS." .
John Lennon