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OPTIMIZATION AND VALIDATION OF THE LIGHTSCANNER® ASSAY FOR THE MCAD DEFICIENCY DISEASE  Sadhaasivam Kamalanaadhan MST Program – Biotechnology Track u0569466
COMPANY BACKGROUND ,[object Object],[object Object],[object Object],[object Object]
INTERNSHIP OBJECTIVES ,[object Object],[object Object],[object Object]
PROJECT BACKGROUND ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
ASSAY WORKFLOW
TECHNICAL BACKGROUND ,[object Object],[object Object],[object Object],[object Object],Source: http://www.path.utah.edu/news/hi-res-dna-melting-analysis/image/image_view_fullscreen
Source:  http://en.wikipedia.org/wiki/File:STD_HRM_plot.JPG
OPTIMIZATION OF ASSAY – PCR CONDITIONS ,[object Object],[object Object],[object Object],[object Object]
AGAROSE GEL ELECTROPHORESIS
OPTIMIZATION OF ASSAY - LIGHTSCANNER ® ,[object Object],[object Object],[object Object],[object Object]
 
VALIDATION - PRECISION ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Within Between
Exons 4 & 5 – within runs
Problematic exons 8 &12 – Within Runs
Instrument Calls Operator Calls n – negative, p – positive, ?n – questionable negative
VALIDATION - ACCURACY ,[object Object],[object Object],[object Object]
SETUP OF ACCURACY RUNS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
OPERATOR ASSISTANCE REQUIRED
 
Instrument Scores Operator Scores
OVERALL – INSTRUMENT VS. OPERATOR ,[object Object],[object Object]
Exon Results
 
 
Can I ever become independent? Someday maybe you will! INSTRUMENT (DEJECTED) OPERATOR (REASSURING)
VALIDATION SUMMARY FOR EXONS
DISCUSSION AND RECOMMENDATIONS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
INTERNSHIP OBJECTIVES ,[object Object],[object Object],[object Object]
MUTATION DATABASE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
INTERNSHIP OBJECTIVES ,[object Object],[object Object],[object Object]
PRICING COMPONENTS
COST ACCOUNTING ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
COMPETITOR ANALYSIS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
SAMPLE COMPETITOR ANALYSIS REPORT
PRICING SPREADSHEET - EXAMPLE
CLIENT PRICING
CONCLUSION – THE STORY OF AN ASSAY
THANK YOU

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Editor's Notes

  1. Custom animation effects: faded zoom entrance and exit (Basic) To reproduce the picture effects on this slide, do the following: On the Home tab, in the Slides group, click Layout , and then click Blank . On the Insert tab , in the Illustrations group, click Picture . In the Insert Picture dialog box, select a picture, and then click Insert . On the slide, select the picture. Under Picture Tools , on the Format tab, in the bottom right corner of the Size group, click the Size and Position dialog box launcher. In the Size and Position dialog box, on the Size tab, resize or crop the picture as needed so that under Size and rotate , the Height box is set to 5.5” and the Width box is set to 6.5” . Resize the picture under Size and rotate by entering values into the Height and Width boxes. Crop the picture under Crop from by entering values into the Left , Right , Top , and Bottom boxes. Select the picture. Under Picture Tools , on the Format tab, in the Picture Styles group, click Picture Shape , and then under Rectangles click Rounded Rectangle (second option from the left). On the picture, drag the yellow diamond adjustment handle toward the corner to decrease the rounding on the corners of the rectangle. Under Picture Tools , on the Format tab, in the Picture Styles group, click Picture Effects , point to Shadow , and then under Outer click Offset Center (second row, second option from the left). To reproduce the animation effects on this slide, do the following: On the Animations tab, in the Animations group, click Custom Animation . On the slide, select the picture. In the Custom Animation task pane, click Add Effect , point to Entrance , and then click More Effects . In the Add Entrance Effect dialog box, under Subtle , click Faded Zoom , and then click OK . Under Modify: Faded Zoom , in the Start list, select With Previous . Under Modify: Faded Zoom , in the Speed list, select Medium . Also in the Custom Animation task pane, click Add Effect , point to Exit , and then click More Effects . In the Add Exit Effect dialog box, under Subtle , click Faded Zoom , and then click OK . Under Modify: Faded Zoom , in the Start list, select On Click . Under Modify: Faded Zoom , in the Speed list, select Medium . To duplicate the animated picture effects on this slide, do the following: Select the first picture. On the Home tab, in the Clipboard group, click the arrow under Paste , and then click Duplicate . Select the second picture. Under Picture Tools , on the Format tab, in the Adjust group, click Change Picture . In the Insert Picture dialog box, select a picture, and then click Insert . Select the second picture. Under Picture Tools , on the Format tab, in the bottom right corner of the Size group, click the Size and Position dialog box launcher. In the Size and Position dialog box, on the Size tab, resize or crop the picture as needed so that under Size and rotate , the Height box is set to 5.5” and the Width box is set to 6.5” . Resize the picture under Size and rotate by entering values into the Height and Width boxes. Crop the picture under Crop from by entering values into the Left , Right , Top , and Bottom boxes. Select the second picture. On the Home tab, in the Clipboard group, click the arrow under Paste , and then click Duplicate . Select the third picture. Under Picture Tools , on the Format tab, in the Adjust group, click Change Picture . In the Insert Picture dialog box, select a picture, and then click Insert . Select the third picture. Under Picture Tools , on the Format tab, in the bottom right corner of the Size group, click the Size and Position dialog box launcher. In the Size and Position dialog box, on the Size tab, resize or crop the picture as needed so that under Size and rotate , the Height box is set to 5.5” and the Width box is set to 6.5” . Resize the picture under Size and rotate by entering values into the Height and Width boxes. Crop the picture under Crop from by entering values into the Left , Right , Top , and Bottom boxes. Press and hold CTRL, and then select all three pictures. On the Home tab, in the Drawing group, click Arrange , point to Align , and then do the following: Click Align to Slide . Click Align Middle . Click Align Center . In the Custom Animation task pane, select the sixth animation effect (faded zoom exit effect for the third picture). Click the arrow to the right of the effect, and then click Remove . To reproduce the background effects on this slide, do the following: Right-click the slide background area, and then click Format Background . In the Format Background dialog box, click Fill in the left pane, select Gradient fill in the Fill pane, and then do the following: In the Type list, select Radial . Click the button next to Direction , and then click From Center (third option from the left). Under Gradient stops , click Add or Remove until two stops appear in the drop-down list. Also under Gradient stops , customize the gradient stops as follows: Select Stop 1 from the list, and then do the following: In the Stop position box, enter 0% . Click the button next to Color, and then under Theme Colors click Black, Text 1, Lighter 50% (second row, second option from the left). Select Stop 2 from the list, and then do the following: In the Stop position box, enter 100% . Click the button next to Color , and then under Theme Colors click Black, Text 1 (first row, second option from the left).