1. DINESH KUMAR
08882170241 use
Dineshsinghrajpoot11@gmail.com
Dineshsinghrajpoot1@yahoo.com
POST APPLIED :- Diagnostic speacilist / application speacilist Flowcytomery & Cancer Research
/ Sr.Lab Tech Flowcytometry and hematology
Instument- BD FACS Canto 11 ( 10 color panel used )
CAREER OBJECTIVE:
 To work as a qualified and successful Diagnostic speacilist of flowcytometry in a
motivating and competitive environment where I can apply my skills and learn newer
techniques to upgrade myself to provide effectiveness in the relevant field.
 flow cytometry services for clinical laboratory support, beginning with cytometer
maintenance, troubleshooting and FACS DiVa database management. Maintained
large inventory of CAP-compliant 8-color cocktails for surface and intracellular
staining, with targets including cytoplasmic kappa and lambda, ZAP-70, and TdT.
Performed re-compensations where indicated following voltage adjustments or
tandem dye breakdown. Acquired titration data using DiVa batch processes.
Analyzed performance data for new equipment purchases, using multispectral
beads. Coordinated change of platform for flow assays, from 8-color BD Cantos to
10-color , Managed QC documentation for cytometer performance on Gallios and
Cantos. Authored new reporting templates in FCS Express for comparison of new
antibodies and all other laboratory developed tests. Wrote SOPs and trained
technical staff. Services were provided to a top-five clinical reference lab, with
high-throughput case loads and <24 hour turn-around times
Practical session cover-:
operate, maintain and troubleshoot the instrument
setup the instrument by selecting relevant controls for performance, compensation and
gating
design multicolor experiments up to 10 colors
adapt default sorting settings to optimize speed, viability, purity and efficiency
perform optimized sorts on different applications including various samples and rare
cell populations
Date management and data acquisition using the BD FACS Sortware software
Multicolor gating and compensation
Standard operation and maintenance procedures
Multicolor data acquisition and sorting strategies
Data collection, gating and compensation with FACS CANTO / FACD DIVA ware software
Tips for troubleshooting, sample preparation and handling
Tips for troubleshooting, sample-preparation and
Overview on fluorescent dyes & compensation

2. Overview on fluorescent dyes, spectral spill over & compensation
Set up and Quality Control of BD Flow Cytometers
“Fluorogame” to memorize the different fluorochrome characteristics
Compensation
Data recording and analysis of eight-color experiments
Multicolor Panel design exercises
Instrument Quality control (QC) using CS&T
Sample preparation & optimization
Acquisition and analysis
Set up and Quality Control of BD Flow Cytometers for optimized multicolor experiments
Compensation theory for multiparameter analysis
Optimizing choice of antibody/fluorochrome when performing staining of both surface
markers and intracellular proteins
an analysis tool
Discussion and of results
Theoretical presentation include-:
Set up and Quality Control of BD Flow Cytometers
Overview on fluorescent dyes, spectral spill over & compensation
Characteristics and combination of fluorochromes
Prevention of artefacts in Muticolor Flow cytometry: Compensation related artefacts
Multicolor panel design
Multicolor experiments for intra-cellular staining
Negative controls
Tips for troubleshooting, sample-preparation and
Set up and Quality Control of BD Flow Cytometers for optimized multicolor experiments
Compensation theory for multiparameter analysis
Optimizing choice of antibody/fluorochrome when performing staining of both surface
markers and intracellular proteins
an analysis tool
Discussion and of results
Basic concepts of flow cytometry and cell sorting
Main parts and operational modes
Instrument specific optics, fluidics and maintance
Overview on fluorescent dyes, spectral spillover & compensation
PROFESSIONAL QUALIFICATION:
 BSc. in ( Bsc.Pathology ) from Combined Institute of Medical Science and Research
Centre (CIMS), affiliated to HNB Garhwal University, Uttarakhand,UK India.

3. CLINICAL INTERNSHIP:
 Presently working as a senior flowcytometry & Hematology tech in action cancer hospital
pachim vihar new delhi 21 april 2015 to till date
 Completrly new set up validation and statnratation & opening of flowcytometry self based
 worked as a Diagnostic speacilist of flowcytometry and cancers research in Dabur
OncQuest Laboratories Ltd. (CAP, NABL Certified Lab ) in New Delhi From 14th Nov
2010 . To 25 april 2015
 Six months Clinical Internship at MAX Super specialty hospital (under Devki Foundation),
Saket, Delhi & flow cytometry immunophenotype
FAMILIAR MACHINES:
 Sysmex-KX21
 Sysmex XN-1000
 Flocytometry Analyzer
Standard operation and maintenance procedures
Multicolor data acquisition and sorting strategies
Data collection, gating and compensation with FACS CANTO / FACD DIVA software
Tips for troubleshooting, sample preparation and handling
Tips for troubleshooting, sample-preparation and
Overview on fluorescent dyes & compensation
Overview on fluorescent dyes, spectral spill over & compensation
Set up and Quality Control of BD Flow Cytometers
 BD FACSCanto ‫ןן‬
JOB RESPONSIBILITIES :
 Processing acquisation & validation of all Leukemia/Lymphoma, CD3/CD4/CD8,CD34
Enumeration and DNA ploidy samples.
 Acquisition and analysis & validation of Leukemia/ Lymphoma Sample.
 Acquisition and analysis & validation of ALL MRD
 Acquisition and analysis & validation of PNH on granulocytes and RBCs.
 Acquisition and analysis DNA Ploidy (Solid Tumor Tissue, Blood )
 Acquisition and analysis & validation of CD34 enumeration
 Acquisition and analysis & validation of multipal myeloma
 To verify that non-conforming investigation are properly evaluated and take preventive
action to eliminate them.
 To maintain documented procedures (SOP) and records for the inspection and
verification.
 To ensure appropriate storage, preservation of reagent chemicals and supervise the
release of reports.
 To ensure that calibration of the equipments is true and accurate.
 To ensure that material of specified qualities are procured.
 To maintain the QC and Stock Management.

4. ACADEMIC QUALIFICATION:
DEGREE INSTITUTE/BOARD YEAR PERFORMANCE
Bsc.Pathology Combined Institute of Medical
Science and Research Centre
(CIMS), affiliated to HNB Garhwal
University, Uttarakhand, India.
2010 66%
10+2 UP Board 2006 54%
10th
UP Board 2004 63%
CONFERENCE ATTENDED:
 Chandigarh Chapter & Scientific Seminar on World Biomedical Laboratory Science Day
0n 15th April 2009 at PGIMER, Chandigarh, India.
 Chandigarh Chapter & Scientific Seminar on World Biomedical Laboratory Science Day
0n 15th April 2010 at PGIMER, Chandigarh, India.
PERSONAL DOSSIER:
Dinesh Kumar
S/O Shri Fateh Singh
DOB:04-06-1988
Status: Male
Linguistic skills: Hindi, English
Status- Marrird
Address
Village-Bas Jharna
Post- Dhirpura (Tundla)
Dist- Firozabad
State- Utter Pradesh
Pin-283204
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