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Complements of HydroTec Systems for further information call 815-624-6644 or e-mail us at info@purehts.com.

International Organization for Standardization specification for water for laboratory use ISO 3696: 1987
Parameter:

Grade 1

Grade 2

Grade 3

N/A

N/A

5.0 to 7.5

pH value at 25ºC inclusive range
Electrical conductivity µS/cm 25ºC, max.

0.1

1

5

Oxidizable matter Oxygen (O2) content mg/L max.

N/A

0.08

0.4

Absorbance at 254 nm and 1 cm optical path length, absorbance units, max.

0.001

0.01

Not
specified

Residue after evaporation on heating at 110ºC mg/kg, max.

N/A

1

2

Silica (SiO2) content mg/L, max.

0.01

0.02

Not
specified

American Society for Testing and Materials (ASTM) D1193-91

Type I*

Electrical Conductivity Max. (µS/cm @ 25ºC)

Type II **

Type III***

Type IV

0.056

1

0.25

5

18

1

4

0.2

Electrical Resistivity
Min. (MΩ-cm @ 25ºC)
pH @ 25ºC

-

TOC max. (µ g/L)

-

-

5.0 - 8.0

100

50

200

Sodium max (µg/L)

1

5

10

Silica max. (µg/L)

3

3

500

Chloride max. (µg/L)

1

5

10

Key:
*Requires the use of 0.2µm membrane filter
** Prepared by distillation
*** Requires the use of a 0.45µm membrane filter
bacterial levels need to be controlled, reagent grade types should be further classified as follows:
Type A
Total Bacterial Count max. CFU/100 ml
Endotoxin max. IU/ml

Type B
1
0.03

Type C
10
0.25

1000
-

National Committee for Clinical Laboratory Standards (NCCLS) (1988)

Type I

Type I I

Type III

Bacteria (CFU/ml)

< 10

< 1000

NA

pH

NA

NA

5.0 - 8.0

Resistivity (MΩ-cm @ 25ºC)

> 10*

>1

> 0.1

SiO2 mg/L

< 0.05

< 0.1

<1

Total Solids mg/L
Total Oxidizable Organic Carbon mg/L

0.1

1

< 0.05

< 0.2

5
<1

Type I water must be free of particulate matter larger than 0.2µm
* Resistivity of Type I must be measured in-line
Pharmacopoeia requirements for purity of 'purified water'
Properties

EP

USP

Nitrates

<0.2 ppm

-

Heavy metals

<0.1 ppm

-

TOC

<500 µg/L C

<500 µg/L C

Conductivity

<4.3 µS/cm at <1.3 µS/cm at 25ºC
20ºC

Bacteria (guideline)

<100 CFU/ml <100 CFU/ml

Additional Standards: Water For Injection -- Standard Endotoxins

Assume same 0.25 EU/mL
as USP

No limit
50
No limit
50

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Caldad del agua

  • 1. Complements of HydroTec Systems for further information call 815-624-6644 or e-mail us at info@purehts.com. International Organization for Standardization specification for water for laboratory use ISO 3696: 1987 Parameter: Grade 1 Grade 2 Grade 3 N/A N/A 5.0 to 7.5 pH value at 25ºC inclusive range Electrical conductivity µS/cm 25ºC, max. 0.1 1 5 Oxidizable matter Oxygen (O2) content mg/L max. N/A 0.08 0.4 Absorbance at 254 nm and 1 cm optical path length, absorbance units, max. 0.001 0.01 Not specified Residue after evaporation on heating at 110ºC mg/kg, max. N/A 1 2 Silica (SiO2) content mg/L, max. 0.01 0.02 Not specified American Society for Testing and Materials (ASTM) D1193-91 Type I* Electrical Conductivity Max. (µS/cm @ 25ºC) Type II ** Type III*** Type IV 0.056 1 0.25 5 18 1 4 0.2 Electrical Resistivity Min. (MΩ-cm @ 25ºC) pH @ 25ºC - TOC max. (µ g/L) - - 5.0 - 8.0 100 50 200 Sodium max (µg/L) 1 5 10 Silica max. (µg/L) 3 3 500 Chloride max. (µg/L) 1 5 10 Key: *Requires the use of 0.2µm membrane filter ** Prepared by distillation *** Requires the use of a 0.45µm membrane filter bacterial levels need to be controlled, reagent grade types should be further classified as follows: Type A Total Bacterial Count max. CFU/100 ml Endotoxin max. IU/ml Type B 1 0.03 Type C 10 0.25 1000 - National Committee for Clinical Laboratory Standards (NCCLS) (1988) Type I Type I I Type III Bacteria (CFU/ml) < 10 < 1000 NA pH NA NA 5.0 - 8.0 Resistivity (MΩ-cm @ 25ºC) > 10* >1 > 0.1 SiO2 mg/L < 0.05 < 0.1 <1 Total Solids mg/L Total Oxidizable Organic Carbon mg/L 0.1 1 < 0.05 < 0.2 5 <1 Type I water must be free of particulate matter larger than 0.2µm * Resistivity of Type I must be measured in-line Pharmacopoeia requirements for purity of 'purified water' Properties EP USP Nitrates <0.2 ppm - Heavy metals <0.1 ppm - TOC <500 µg/L C <500 µg/L C Conductivity <4.3 µS/cm at <1.3 µS/cm at 25ºC 20ºC Bacteria (guideline) <100 CFU/ml <100 CFU/ml Additional Standards: Water For Injection -- Standard Endotoxins Assume same 0.25 EU/mL as USP No limit 50 No limit 50