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1. Parkin is an E3 ubiquitin ligase. Which of the following is/are parkin substrates?
a. aSp22 form of alpha-synuclein
b.Pael-R
c.Synphilin
d. Cyclin E
e. All of the above
2.a-synuclein can be found to migrate at 22 kDa while it's predicted molecular weight is 16
kDa.This is due to...
a.proteolytic processing
b.ubiquitination
c.phosphorylation
d.O-glycosylation
e. N-glycosylation
3. What are the minimal components needed for an in vitro ubiquitin ligase assay to poly-
ubiquitinate substrate?
a.E1, E2, IP-parkin, substrate
b.E1, E2, IP-parkin, parkin peptide, ubiquitin
c. E1, E2, IP-parkin, ubiquitin
d. E1, E2, IP-parkin

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1 Parkin is an E3 ubiquitin ligase Which of the following.pdf

  • 1. 1. Parkin is an E3 ubiquitin ligase. Which of the following is/are parkin substrates? a. aSp22 form of alpha-synuclein b.Pael-R c.Synphilin d. Cyclin E e. All of the above 2.a-synuclein can be found to migrate at 22 kDa while it's predicted molecular weight is 16 kDa.This is due to... a.proteolytic processing b.ubiquitination c.phosphorylation d.O-glycosylation e. N-glycosylation 3. What are the minimal components needed for an in vitro ubiquitin ligase assay to poly- ubiquitinate substrate? a.E1, E2, IP-parkin, substrate b.E1, E2, IP-parkin, parkin peptide, ubiquitin c. E1, E2, IP-parkin, ubiquitin d. E1, E2, IP-parkin