ppt

1. Fungi causing Subcutaneous Zygomycosis.
Zygomycos
is
Order
Mucorales
Order
Entomophtho
rales
Acute Chronic

2. 1. Zygomycosis (Mucorales).
Mucorales infections Definition:
•Angiotropic (blood vessel-invading)
•The most common genera causing disease are:
Rhizopus
Rhizomucor
Mucor
Absidia
•Fast growing non-septate molds

3. Geographical distribution & normal habitat
•World-wide
•Soil and decomposing organic matter
•Found in outdoor and indoor air
•Food and dust
Clinical forms
The infection typically involves the:
•Rhino-facial-cranial area
•Lungs, gastrointestinal tract or skin
•Other parts can also be affected

4. Risk factors
•The disease is associated with:
Diabetic ketoacidosis
Malnourished children
Severely burned patients
•It is also seen in patients with
Leukemia
Lymphoma
AIDS
In patients using corticosteroids

5. Laboratory diagnosis
•Specimens:
Aspirated material from sinuses
Sputum in pulmonary disease
Biopsy material
Management of mucormycosis
•The prognosis is bad
•Most cases of gastric and pelvic disease are diagnosed at
autopsy
•Cases occurred in patients with pulmonary disease, leukemia, or
with lymphomas, are usually fatal
•Control of the diabetes, aggressive surgical debridement of
involved tissue, and high doses of Amphotericin B are
recommended

6. 2- Zygomycosis
Order
Entomophthorales
Entomophthorales infections
•Causes subcutaneous zygomycosis
•Known as Entomophthoromycosis
•Tow genera are involved:
Conidiobolus
Basidiobolus
•Infections are:
Chronic
Slowly progressive
Restricted to the subcutaneous tissue

7. •Not like Mucorales:
No vascular invasion or infarction
Chronic inflammatory response
Basidiobolus infection
•Chronic inflammatory or granulomatous disease
•Subcutaneous tissue of the limbs, chest, back or buttocks
•Mostly in children (predominance in males)

8. Conidiobolus infection
•Chronic inflammatory or granulomatous disease
•Nasal submucosa
•Characterized by polyps or palpable subcutaneous masses
•Occur mainly in adult (80% of cases)
Laboratory diagnosis
•Specimens:
1.Aspirated material from sinuses
2.Biopsy material

9. The following steps are used for the both types of
Zygomycosis:
1.10 or 20% KOH:
1.Typically contain thick-walled aseptate hyphae
2.Swollen cells (up to 50 um) and distorted hyphae
may be present
2. Culture:
1.SDA without cycloheximide at 30°C
2. Rapid growth
3- A diagnosis can also be made by examining a biopsy
for granuloma formation and the presence of aseptate
hyphae, eosinophils, neutrophils, and fibroblasts. Tissue
for culture should be send in a dry sterile container, and
biopsies should be fixed and send in formal saline.

10. Sporangium containing sporangiospores

11. Spherical, brown zygospores formed
between opposite suspensors. Also,
present sporangiola.

12. Typical colony on Sabouraud glucose agar
at 25C

13. Hyphae in lung tissue

14. Hyaline sparsely septate hyphae biopsy
specimen of palate.

15. A peach, which has been colonized
by Rhizopus sp

16. A gastric proliferative mycosis in a chicken
caused by Rhyzopus arrhizus

17. Wide, sparsely-septate hyphae and
striated sporangiospores

18. Non-septate (coenocytic) hyphal strand
characteristic of the fungi belonging to the
Zygomycetes