Match the Statements below with bacteriological tests. Eosin-methyle.pdf

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Many non-scientists have focused their criticism of evolution on the fact that even scientists have criticized Darwin’s theory. Is this true? In what respect did Darwin get it wrong, or maybe in what way has Darwin’s theory been modified? Solution The \"Origin of Species\" of Darwin described about the heritable change in an organism over a period of time due to natural selection. Scientists also criticized of Danwin\'s opinion on use and disuse theory. At the time of Origin of species, Darwin do not know about the existed of microorganisms and his theory of tree of life became wrong. Pangenesis theory of Darwin also became wrong as he do not know anything about genetics..

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Match the Statements below with bacteriological tests. Eosin-methylene blue Kovac's reagent
reaction H_2O_2 is used Contains mannitol sugar Metabolism of tryptophan Ammonia causes
color change Contains high concentration of sodium chloride Staphylococcus aureus changes it
to yellow The main carbohydrate for these tests is lactose Sulfanilic acid and
dimethylnaphthalamine are reagents Contains lactose, sucrose, glucose Green metallic sheen
with E. coli Hydrogen sulfide is produced Acid slant, acid butt is the reaction Contains Bile
salts Anaerobic organisms This medium is used to measure hemolysis Zinc is a reagent in this
test Vacomycin, colisting and nystatin are inhibitors in this medium Crystan violet and lactose
Detects cytochromes in bacteria Urease test TSI MSA ENB SIM Catalase test MacConkey
agar Simmon citrate Oxidase test Blood agar Chocolate agar Nitrate test Thioghlycolate
medium
Solution
Answer:
(i) d
Eosin methylene blue agar is abbreviated as EMB.
(ii) e
Kovac's reagent is used in indole test on SIM (Sulfide, Indole, Motility) medium.
(iii) f
Presence of catalase is detected using hydrogen peroxide.
(iv) c
Mannitol salt agar is a selective and differential growth media containing mannitol.
(v) e
SIM media is a differential media which contains tryptophan. If the test organism contains
tryptophanase, tryptophan gets degraded.
(vi) a
Urease converts urea to ammonia to bicarbonate in urease test.
(vii) c
MSA contains 75.0 g/L sodium chloride.
(viii) c
Staphylococcus ferments mannitol and changes medium turns yellow.
(ix) g
MacConkey agar turns ping if the organism ferments lactose.
(x) l
Nitrate test is used to test for reduction of nitrate (NO3–) to nitrite (NO2–) by adding sulfanilic

acid reagent and diphenylnaphthylamine.
(xi) b
TSI slant contains agar, phenol red, lactose, sucrose, glucose.
(xii) d
If E. coli grows on EMB, it will give a distinctive metallic green sheen.
(xiii) b
Hydrogen sulphide is produced in TSI slant when sugars get fermented.
(xiv) b
Acid slant, acid butt reaction is observed in TSI slant.
(xv) g
MacConkey agar contains bile salt to inhibit gram positive bacteria.
(xvi) i
Oxidase test is used to detect cytochrome system in bacteria. Anaerobic organisms lack
cytochrome systems.
(xvii) j
Blood agar is used to detect hemolytic enzymes a bacteria possesses.
(xviii) l
Zinc powder is uded in nitrate test to detect the nitrate reduction enzyme in bacteria on the basis
of colour.
(xix) k
Modified Thayer Martin agar is based on Chocolate II agar for the selective isolation and growth
of Neisseria species.
(xx) g
MacConkey agar contains lactose and crystal violet dye to selectively isolate gram negative and
enteric bacilli.
(xxi) f
Catalase is present in bacteria with cytochromes, while it lacks in bacteria where cytochrome is
absent. Bacteria lacking cytochrome system cannot breakdown hydrogen peroxide due to
absence of catalase.

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