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A culture of yeast was grown in a media containing sucrose labeled by 14C at 2nd position of
fructose. Determine the localization of the label in palmitic acid synthesized by the yeast.
Solution
GLYCOLYSIS result in synthesis of acetyl CoA
acetyl CoA formed from fructose will be labeled by 14C as 2nd position of fructose is labeled by
14C
this will result in sub cellular localisation of pamitic acid synthesis......by acetyl CoA which is
labeled by 14C

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