Conclusion: Phases of Oxidative Phosphorylation Focus your attention on the two phases of oxidative phosphorylation in Focus Figure 24.8. Sort the events into the appropriate phase of oxidative phosphorylation. Events may be sorted to only one bin. Solution Oxidative phosphorylation is the process where energy is harnessed through a series of protein complexes embedded in the inner membrane of mitochondria to create ATP. NADH donates e-During breakdown of glucose ,a large amount of NADH and FADH2 are produced in glycolysis and citric acid cycle NADH transfers transfers its high energy molecules to protein complex1 and causes loss of electrons NADH -> NAD++H++2e- Generation of protongradient The process of transferring of electrons drives the pumping of protons and it generates proton gradient across the inner mitochondrial membrane Transfer of electrons Electrons transfers between specalized proteins embedded in the inner mitochondrial membrane. Generation of Water At the end of the electron transport chain ,electrons are transferred to molecular oxygen,which splits in half and takes up H+ to form water 1/2O2+2H++2e-->H2O Synthesis of ATP This proton pumping that is ultimately responsible for coupling the oxidation and reduction reaction to ATP synthesis from ADP and HPO42-.Phosphorylation of ADP and synthesis of ATP occurs Oxygen is the final electronacceptor Electrons move from one carrier to another and finally transferred to o2 Chemiosmosis The diffusion of hydrogen ions across the membrane via ATP synthase due to proton gradient that forms on the otherside of the membrane Flow of proton intomitochondrial martrix ATP synthetase allows H+ to diffuse back into matrix Phosphorylation of ADP ATP synthetase allows H+ ions to diffuse back into the matrix and uses the free energy released to synthesize ATP from ADP and HPO42- Oxidation of food fuels To make ATP,energy must be obsorbed it is supplied by the food we eat.One of the principal energy yielding nutrients in our diet is glucose.The complete breakdown of glucose into CO2 occurs in two process glycolysis and citric acid cyclePhase 1Phase2Neither NADH donates e-During breakdown of glucose ,a large amount of NADH and FADH2 are produced in glycolysis and citric acid cycle NADH transfers transfers its high energy molecules to protein complex1 and causes loss of electrons NADH -> NAD++H++2e- Generation of protongradient The process of transferring of electrons drives the pumping of protons and it generates proton gradient across the inner mitochondrial membrane Transfer of electrons Electrons transfers between specalized proteins embedded in the inner mitochondrial membrane. Generation of Water At the end of the electron transport chain ,electrons are transferred to molecular oxygen,which splits in half and takes up H+ to form water 1/2O2+2H++2e-->H2O Synthesis of ATP This proton pumping that is ultimately responsible for coupling the oxidation and reduction reaction to ATP synthesis from ADP .

1. Conclusion: Phases of Oxidative Phosphorylation Focus your attention on the two phases of
oxidative phosphorylation in Focus Figure 24.8. Sort the events into the appropriate phase of
oxidative phosphorylation. Events may be sorted to only one bin.
Solution
Oxidative phosphorylation is the process where energy is harnessed through a series of protein
complexes embedded in the inner membrane of mitochondria to create ATP.
NADH donates e-During breakdown of glucose ,a large amount of NADH and FADH2 are
produced in glycolysis and citric acid cycle
NADH transfers transfers its high energy molecules to protein complex1 and causes loss of
electrons
NADH -> NAD++H++2e-
Generation of protongradient
The process of transferring of electrons drives the pumping of protons and it generates proton
gradient across the inner mitochondrial membrane
Transfer of electrons
Electrons transfers between specalized proteins embedded in the inner mitochondrial membrane.
Generation of Water
At the end of the electron transport chain ,electrons are transferred to molecular oxygen,which
splits in half and takes up H+ to form water
1/2O2+2H++2e-->H2O
Synthesis of ATP
This proton pumping that is ultimately responsible for coupling the oxidation and reduction
reaction to ATP synthesis from ADP and HPO42-.Phosphorylation of ADP and synthesis of ATP
occurs
Oxygen is the final electronacceptor Electrons move from one carrier to another and finally
transferred to o2
Chemiosmosis
The diffusion of hydrogen ions across the membrane via ATP synthase due to proton gradient
that forms on the otherside of the membrane
Flow of proton intomitochondrial martrix
ATP synthetase allows H+ to diffuse back into matrix
Phosphorylation of ADP
ATP synthetase allows H+ ions to diffuse back into the matrix and uses the free energy released
to synthesize ATP from ADP and HPO42-

2. Oxidation of food fuels
To make ATP,energy must be obsorbed it is supplied by the food we eat.One of the principal
energy yielding nutrients in our diet is glucose.The complete breakdown of glucose into CO2
occurs in two process glycolysis and citric acid cyclePhase 1Phase2Neither
NADH donates e-During breakdown of glucose ,a large amount of NADH and FADH2 are
produced in glycolysis and citric acid cycle
NADH transfers transfers its high energy molecules to protein complex1 and causes loss of
electrons
NADH -> NAD++H++2e-
Generation of protongradient
The process of transferring of electrons drives the pumping of protons and it generates proton
gradient across the inner mitochondrial membrane
Transfer of electrons
Electrons transfers between specalized proteins embedded in the inner mitochondrial membrane.
Generation of Water
At the end of the electron transport chain ,electrons are transferred to molecular oxygen,which
splits in half and takes up H+ to form water
1/2O2+2H++2e-->H2O
Synthesis of ATP
This proton pumping that is ultimately responsible for coupling the oxidation and reduction
reaction to ATP synthesis from ADP and HPO42-.Phosphorylation of ADP and synthesis of
ATP occurs
Oxygen is the final electronacceptor Electrons move from one carrier to another and finally
transferred to o2
Chemiosmosis
The diffusion of hydrogen ions across the membrane via ATP synthase due to proton gradient
that forms on the otherside of the membrane
Flow of proton intomitochondrial martrix
ATP synthetase allows H+ to diffuse back into matrix
Phosphorylation of ADP
ATP synthetase allows H+ ions to diffuse back into the matrix and uses the free energy released
to synthesize ATP from ADP and HPO42-
Oxidation of food fuels
To make ATP,energy must be obsorbed it is supplied by the food we eat.One of the principal
energy yielding nutrients in our diet is glucose.The complete breakdown of glucose into CO2
occurs in two process glycolysis and citric acid cycle