Impact of Genetically Modified Food on Human Health

Middle East Journal of Applied Science & Technology (MEJAST)
(Peer Reviewed International Journal) Volume 2, Issue 3, Pages 75-86, July-September 2019
75 | P a g e ISSN (Online): 2582- 0974 Website: www.mejast.com
Impact of Genetically Modified Food on Human Health
Muhammad faisal1
, Muhammad Saeed1
, Misbah Aslam1
& Muhammad Asif Raheem1
*
1
*Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan-60880, Pakistan.
Article Received: 21 February 2019 Article Accepted: 15 July 2019 Article Published: 30 August 2019
INTRODUCTION
Abbreviation
GMOs: Genetically modified organisms
B.t.: Bacillus thuringiensis
CRISPR: Clustered regularly interspaced short palindromic repeats
FDA: Food and Drug Administration
INTRODUCTION
Genetically modified organisms (GMOs) are those organisms in which the genetic material has been altering which
are not already present in nature and genetically modified foods are those foods derived from animals and the plants
in which genes from particularly desired characteristics are added to an organism DNA. 1
GMOs are used in
various ways such as used in biological and medical research, production of pharmaceutical drugs, experimental
medicine, and agricultural field. By the gene alteration method, we are improving the needs of food and also it
improves the quality of the food.2
We can get our desired characters or goals in plants and animals (such as
resistance to disease, improve shelf life) more quickly as compared to using traditional methods. (Verma 2011) But
many ethical issues are also opened which describe the negative effect of the production of genetically modified
foods. It shows that the insertion of the gene of interest for desired characters in the food may be protective but may
cause high expression of such genes related to many diseases such as chronic.4
METHODS OF PRODUCTION OF GM FOODS
Several methods are known for the production of GMOs from which we get the food. The gene of interest which
inserted into the cell of microorganisms, plants or animals is called a transgene. It is consolidated into the genome
of the recipients which are called transgenic. The transgenes are genes with known traits or mutated variants of
known genes. There many direct and indirect methods of gene transformation are known these days. 1,5
ABSTRACT
Any nutritious substance that people or animals eat or drink or those plants absorb in order to maintain life and growth is called Food. With the huge
increasing population of the world, food production from natural resources could not meet their needs. So researchers move to produce more food
using molecular-level techniques. This type of food is called genetically modified food (GM food), whose genetic material has been altered which is
not present already in nature. GM food is made to increase nutrient content by alternation, has many advantages for humans as it increases the
nutritional content and formation of pest, drought, herbicide, and cold resistant plants. But at the same time, it has negative impacts also. It is
genetically unsafe, causing organ damage and allergic reactions in the digestive tract. The researchers are trying to do their best to produce crops with
their desired characteristics by using molecular-level techniques.
Keywords: Genetically modified organisms, Transformation, Food crops, Bacillus thuringiensis.

PHYSICAL METHODS
S.No
1. Microinjection 6. Laser-mediated
2. Macro-injection 7. Silica/Carbon fibers
3. Pressure 8. Electrofusion
4. Biolistic-gene gun 9.. Electroporation
5. Protoplast 10. Particle bombardment
CHEMICAL METHODS
S.No.
1. Polyethene glycol, 4. Protein
2. Liposome-mediated transfer 5. Artificial lipid
3. Dendrimers 6. Calcium phosphate
BIOLOGICAL METHODS
S.No.
1. Agrobacterium-mediated
2. Virus-mediated
6,7,16,8–15

ELECTROPORATION
Electroporation is a physical phenomenon in which tiny pores are formed in the cell membrane by applying a
sufficiently high electric field. (Bennett et al. 2014; Delalande et al. 2015) These pores are enough for the transport
for a broad range of molecules such as DNA into and out of the cell. It is a reversible and irreversible method. This
occurs in the lipid bilayer of the membrane of all prokaryotes and eukaryotic cells. This method is first reported for
excitable cells in 1958 and no excitable cells in 1967, for planer lipid bilayers in 1979, and for lipid vesicles in
1981. 6,19
The preparation of soymilk (when soybean washed and soaked overnight and blended with water) is an
example of electroporation in which cell culture exposed to an electric field. 20
MICROINJECTION
Microinjection is a physical cell transfer method in which foreign genetic material (gene of interest) directly inject
into cell parts. 5,21–23
Nowadays, we developed an automated micropipette based quantitative microinjection
technology. By this technology precise amount of material delivered into the cell. 24
Other several microinjection
systems are also introduced which based on robotic technology to enable automated injection which high
transfection efficiency. 20,25,26
AGROBACTERIUM-MEDIATED TRANSFORMATION
It is a biological method of transfer the gene of interest into the plant genome. Agrobacterium tumefaciens is gram
negative soil bacteria which cause disease in the plant (many woody and some herbaceous plant) called “Crown
gall disease” through its Ti plasmid which causes plant tumor. A complex process is involved in the integration of
T-DNA (Ti plasmid). 8,27–30
By replacing the Ti plasmid which causes infection we can solve the problem of the
tumor in the plant. The 1st
transgenic cotton has obtained by this method. 31,32
WHY GM FOOD NEEDED?
The word GM food is used for such crops which are formed by genetic engineering. Such plants have been altered
in the laboratory to improving many interesting characteristics such as, disease resistance or to improve nutritional
value. Genetic engineering helps to produce such plant with exact desire trait rapidly. (Verma 2011) For example
by this technique, extract a gene which contains drought tolerance trait and inserts that gene into the different
plants. The new plant will be genetically modified drought tolerance. By increasing the population food demand
also increases 33
. So with the help of GM food, we can increase the quantity of food by using short land but high
productivity is cultivated.34
As the food was deficient many essential nutrients, (vitamins and minerals) for
example, deficiency of Vitamin A causing many diseases such as (liver disorders, eye disorders)? Not only we can
transfer the gene from plant to plant but we can transfer the gene from non-plant to the plants. The best example is
B.t. cotton in which we take the gene from bacterial species, Bacillus thuringiensis and transfer it into the plants
such as corn, cotton, etc.35
SOME GENETICALLY MODIFIED FOOD CROPS
Soybeans, Maize, Sugarcane, Canola, Cotton, Corn, Brinjal, Rice, Banana, Potato, Tomato36,37

SOME GENETICALLY MODIFIED FOOD ANIMALS
Sheep, Goats, Mice, Cow, Fish35
ADVANTAGES OF GM ORGANISMS
1. PEST RESISTANCE
Farmer uses chemical pesticides in a lot of amounts which causes much fetal disease. The consumer never likes to
eat such dangerous food which shows health hazard results. Excessive use of these pesticides and fertilizers causes
water toxicity. By genetic engineering, GM foods grow such as B.t. (Bacillus thuringiensis) corn can help to
eliminate the application of chemical pesticides. 3,38
It’s naturally occurring bacteria which cause the death of the
insect larvae by producing certain types of proteins. The gene of this protein has been transferred into crops which
becomes pest resistance. 39,40
2. HERBICIDE TOLERANCE
Crop plants are genetically engendered to the herbicides which are damaging and reducing the number of crops. For
example, a roundup product has been genetically modified by Monsanto. The 2010 study has found that roundup
formulation causing metabolic disruption in leporinus obtusidens due to long term exposition.41
So by the
production of herbicides tolerance crop, it reduces the production cost, limits the danger application of wheat killer
pesticides. 42,43
Another example is maize which is genetically modified for herbicide tolerant as well as insect
resistance. (Naegeli et al. 2018)
3. DISEASE RESISTANCE
There are many Microorganisms like (viruses, fungi, and bacteria) that cause plant disease. With the help of genetic
engineering, we are trying to create plants that carry the gene that shows tolerance against the above
microorganisms. 3,45
4. COLD RESISTANCE
Due to the cold temperature, the yield of many crops is reduced. By using the genes from cold water fish and
introduce into plants such as potatoes and tobacco. By these genes, plants become cold resistance. 3,46
Barely genes
are used to produce many crops cold resistance as well as all other abiotic stresses and disease resistance.47
5. VIRUS RESISTANCE
Due to the attack of the virus on crops production of the crops reduced. Therefore, genetically modified crops are
produced in a different way to increase production and prevent the attack of viruses. RNA-mediated gene silencing
technique is used to degrade the viral RNA and inactivated the viral DNA by methylation. 42,48
CRISPR/CAS9
technique also use to produce virus resistance crops.49,50
6. DROUGHT TOLERANCE/SALINITY TOLERANCE

By increasing the population of the world, the need for food also increases. Therefore farmers will grow more crops
even in those places where the land is saline or drought. So we are trying to grow drought and saline tolerance crop
plants which can withstand a long period of drought or high salt tolerant content in the soil. 46,51
Nowadays, GM Tomato (Solanum lycopersicum) are produced which are drought and salinity tolerance as well as
confer fast growth and chilling. 52,53
By root engineering, the root size of barley is increased for the capture of
essential nutrients which are important for barley. Due to this drought tolerant of barley increased.54
7. NUTRITION
Malnutrition is common in the 3rd
world countries which are dependent on the single crop as rice. Only rice does not
contain all types of nutritional elements (deficiency of Vitamin A). By the deficiency of the vitamin, blindness is
caused which is a big problem in the 3rd
world countries. If we prepare rice crop which contains additional vitamins
and minerals the problem of malnutrition can be solved. Researchers at the Swiss Federal Institute for plant science
have a created of GOLDEN RICE which contains a high quantity of beta-carotene (vitamin A). By this way, we can
solve the problem of malnutrition in other food crops. 3
Sorghum is another staple food of many countries. Due to
genetic diversity, protein contents in sorghum grain reduced. By the help of Genetic engineering, digestibility and
protein content are increased. (Liu et al. 2019) Potato is another staple food of many countries. By the help of
genetic engineering, many varieties developed in which nutritional components (proteins, lipids, vitamins,
carbohydrates, etc.) are increased. (Bagri et al. 2018) Another many staple food crops are genetically modified.57
And in the orange, beta-carotene increased. (Pons et al. 2014)
A human requires many micro and macronutrient elements for the activity of the body. Due to the deficiency of
even one nutrient, it will result in many diseases (sickness, poor health, immune deficiency in children). Some
essential nutrients are in proteins (Histidine, Lysine, Valine, and Methionine) in lipids (Linoleic acid and Linolenic
acid) in macro-elements (Na, k, Ca, Mg, S, P, and Cl) in micro-elements (Fe, Zn, Mg, As, etc.) in vitamins (A, D, E,
K, C, B1, B2, B3, Niacin, etc.).Genetic engineering is used to improve all these nutrients in a crop. 8,59,60
8. PHARMACEUTICAL
Vaccines are used for the prevention of many infectious diseases. There many problems in the production of the
vaccine, distribution, and delivery. To solve these entire problems, scientist produced such type of vaccine which is
called edible vaccine61
. Food containing health-giving additives called functional food. Researchers working to
develop edible vaccines in fruits like tomato and banana. These edible vaccines are easy to administer than another
injectable vaccine. 42
9. PHYTOREMEDIATION
Many heavy metals (Cu, Pb, and Zn) which are mainly produced by human activities cause the death of plants as
well as essential microorganisms in the soil. 62
With the help of genetic engineering, researchers are trying to design
such plants used in bioremediation (removal of contaminants by using living organisms) such as poplar trees. 63,64

DISADVANTAGES OF GENETICALLY MODIFIED ORGANISMS
The drawback of GM foods includes many environmental problems, human health problems, economical
expensive, unusual taste, trade issue, antibiotic efficiency decrease and maybe cause new disease.65–68
Some other
problems are the following.
1. GMOS ARE INHERENTLY UNSAFE
There are many reasons which show that the GM plants contain unique dangers. By these techniques, GM foods
are prepared by alteration of genes which cause mutations. The genetic material in soybean that makes herbicide
tolerant transformed into the human gut bacteria and continue the function which shows that the eating of GM
crops cause foreign GM proteins to express inside our gut. 3
2. GM DIET CAUSE A CARCINOGENIC REACTION IN THE DIGESTIVE TRACT
The 1st
crop submitted to FDA (food and drug administration) voluntary tomato shows evidence of toxin. Out of 20
female rats that fed GM tomato the seven developed stomach lesion. The type of stomach lesion linked to tomatoes
can lead to life-endangering hemorrhage in a person who uses aspirin to prevent blood clotting. 3
3. GM FOOD CAUSE LIVER DAMAGE
Rats that fed on GM potato had smaller and partially atrophied liver. Rats show liver lesions and other indication
of toxicity when they are fed by GM corn. 69
4. GM FOOD CAUSE ANIMALS DEATH AND ORGAN DAMAGE
The mice fed with roundup ready soy had produce significantly less digestive enzymes and cancer. In rats that fed
with GM potato, there, pancreases were enlarged. GM-fed animals show lesions toxicity altered enzymes
production or inflammation. 370
5. GM CROPS CAUSE AN IMMUNE REACTION AND ALLERGIES
Allergic reactions produced when any foreign particle is detected and interprets with the immune system. All the
GM foods according to their definition have foreign particles that are inserted into the genome. So they provoke
allergic reactions when inter the body. 66,71,72
GM peas provoked and inflammatory response in mice showing that
it may cause a deadly allergic reaction in the people. Scientists found that new protein in GM soybean was able to
bind with IgE antibodies and provoked many dangerous allergic reactions. 73
The B.t. toxins produced by GM
crops (B.t. maize and cotton) are showing a much dangerous toxic reaction than natural verities. 74
APPLICATION OF GM FOOD IN DIFFERENT COUNTRIES
According to a survey of unites states journal, a list of different countries utilizing GM food and their percentage
are given below:

CONCLUSION
The GM food cause beneficial as well as harmful effects on the environment. But everyone somehow is using GM
food in life. It is more preferred because it is using to meet the huge population of the world with healthy and
nutritional food. So it has dominated its harmful impacts and it is used all over the world. It affects human beings
more beneficially than its harms so we try to form more GM food to increase the desired characteristics in the food
by using a gene alteration mechanism. Several laws are present which allow the safe and healthy use of GM food
and limit its harmful effect on the environment.
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