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FSFCP, Sevilla '08




                   Initial Presentation of the Congress


Dear Protistologist,
On behalf of “Grupo Especializado de Protistología” and “Groupment de
Protistologues de Langue Française”, we have the pleasure to announce you
the conjoint celebration in 2008 of their respective meetings. The gathering,
called First Spanish-French Congress on Protistology, will be held in Seville,
from 4th to 6th June. The aim of this meeting is to promote Protistology in
Europe through scientific exchange. Official language is English. We shall
make up an attractive scientific program and encourage the active
participation of young researchers (pre-, post-docs); the city will provide
participants with nice spring weather, history, and charm.
The scientific program will be divided in three main axes, Evolution of
protists, Biology of free-living protists, and Biology of parasitic protists, with
oral presentations and posters. Topics should be set up according to received
abstracts. If financial support allows it, two granted talks, opening and closing
the meeting, each given by an outstanding protistologue during 60 minutes,
are foreseen. Oral communications will be held in morning and afternoon
sessions. Every session will include one talk, given by a senior researcher
during 45 minutes, five secondary talks, given by young researchers during 20
minutes. All submitted abstracts should be considered as potential for oral
presentations; selection will be carried out by the scientific committee of the
meeting. Non-selected abstracts should be presented as posters. A session for
poster discussion will be included also.




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FSFCP, Sevilla '08




                          Organizing Committee

Grupo Especializado     de     Protistología   (GEP),   Sociedad   Española   de
Microbiología (SEM).

Groupement des Protistologues de Langue Française (GPLF).

The Federation of European Protistological Societies (FEPS), a non-profit
association of national and cross-national European Societies of Protistology
and less formal national groups of Protistology of diverse European countries,
supports the meeting

President: Eduardo Villalobo. Departamento de Microbiología, Universidad de
Sevilla. SPAIN. GEP-SEM.

Vice-Presidents: Aurelio Serrano. Instituto de Bioquímica Vegetal y
Fotosíntesis, CSIC, Sevilla. SPAIN. GEP-SEM. Isabelle Desportes Museum
National d'Histoire Naturelle, Paris. FRANCE. GPLF.


                             Scientific Committee

-Dr. Antonio Torres, Universidad de Sevilla, Sevilla. SPAIN.
-Dr Gabriel Gutiérrez, Universidad de Sevilla, Sevilla. SPAIN.
-Dr. Federico Valverde, Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC,
Sevilla. SPAIN.
-Dr. Susana Serrano, Universidad Complutense de Madrid, Madrid. SPAIN.
-Dr. Juan Carlos Gutiérrez, Universidad Complutense de Madrid, Madrid.
SPAIN.
-Dr. Luis Miguel Ruiz Pérez, Instituto de Parasitología y Biomedicina López-
Neyra, CSIC, Granada. SPAIN.
-Dr. Loïc Morin, Université de Paris Sud, Orsay. FRANCE.
-Dr. Anne Baroin-Tourancheau, Université de Paris Sud, CNRS, Orsay.
FRANCE.
-Dr. Purificación López García, Université de Paris Sud, CNRS, Orsay. FRANCE.
-Dr. Nancy Guillén, Institut Pasteur, CNRS, Paris. FRANCE.




                                        3
FSFCP, Sevilla '08




                      Abbreviated Scientific Program

Wednesday, 4th June
-12:00-14:00 h. Registration.
-15:00-15:15 h. Welcome talk by Dr. Eduardo Villalobo (President of the Congress),
Dr. Loïc Morin (President of GPLF), and Dr. Aurelio Serrano (President of GEP-SEM).
-15:15-16:15 h. Opening lecture by Dr. Andrés Aguilera.
AFTERNOON, SESSION I. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF
PARASITIC PROTISTS. Chairperson: Dr. Luis Miguel Ruiz Pérez
-16:30-18:00 h. Oral presentations and discussions.
-18:00-18:30 h. Poster pasting.
-20:00-22:00 h. Welcome cocktail at Patio del Rectorado, Universidad de Sevilla.
Thursday, 5th June.
MORNING, SESSION II. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF FREE-
LIVING PROTISTS. Chairperson: Dr. Geneviéve Bricheux
-09:00-10:00 h. Main talk by Dr Philippe Bouchard.
-10:00-10:30 h. Coffee break.
-10:30-12:30 h. Oral presentations and discussions.
-12:30-13:30 h. Posters discussion session.
I Jornadas Internacionales de Tratamiento y Reutilización de Aguas Residuales: El
Papel de los Protistas (only in spanish). Dr. Susana Serrano and Dr. Blanca Pérez
Uz. Moderadora: Pilar Calvo
-13:30-15:00 h. Lunch and coffee.
AFTERNOON, SESSION III. ECOLOGY AND BIOTECHNOLOGY OF FREE-LIVING PROTISTS.
Chairperson: Dr. Juan Carlos Gutiérrez
-15:00-17:30 h. Conferences on the Role of Protists on Wastewater Treatment and
Reuse.
Dr. Purificación López García, Dr. António Luís Pereira do Amaral and Dr. Lucía
Arregui.
-17:30-19:00 h. Oral presentations and discussions.
-20:30 h. “Tapas tour” in the city centre.
Friday, 6th June
MORNING, SESSION IV. BIOMEDICINE AND BIOTECHNOLOGY OF PARASITIC PROTISTS.
Chairperson: Dr. Federico Valverde
-09:00-10:00 h. Main talk by Dr. Naveed Ahmed Khan.
-10:00-10:30 h. Coffee break.
-10:30-12:00 h. Oral presentations and discussions.
-12:00-13:30 h. Posters discussion session.
-13:30-15:00 h. Lunch and coffee.
AFTERNOON, SESSION V. PROTIST EVOLUTION. Chairperson: Dr. Loïc Morin
-15:00-15:15. Closing talk by Dr. Ernesto García López (Vice-president Sociedad
Española de Microbiología)
-15:15-16:00 h. Main talk by Béatrice Lecroq.
-16:00-18:00 h. Oral presentations and discussions.
-18:00-19:00 h. Closing lecture by Dr. David Moreira.
-20:15-21:15 h Visit to Reales Alcázares.




                                        4
FSFCP, Sevilla '08




                Congress Venue

The Congress will be held in Seville (SW, Spain) at
"ESCUELA    TÉCNICA    SUPERIOR    DE   INGENIERÍA
INFORMÁTICA (ETSII), UNIVERSIDAD DE SEVILLA",
situated in "Campus Reina Mercedes" (Avenida Reina
Mercedes, 41012 Sevilla). We will meet in the "Salón
de Actos" of the "Escuela", with capacity for more
than 300 people, provided with audiovisual media,
and located in the first floor of the building.




           5
FSFCP, Sevilla '08




                       INDEX OF ABSTRACTS BY AUTHORS
OPENING LECTURE
Aguilera, Andrés                                                     9
CLOSING LECTURE
Moreira, David                                                      11
SESSION I. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF
PARASITIC PROTISTS. Chairperson: Luis Miguel Ruiz Pérez
Kohl, Linda                                                         13
López Farfán, Diana                                                 14
Aranguren, Raquel                                                   15
Arroyo, Francisco T.                                                16
Marques, Adam                                                       17
Marques, Adam                                                       18
Weber, Christian                                                    19
SESSION II. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF
FREE-LIVING PROTISTS. Chairperson: Geneviève Bricheux
Bouchard, Philippe                                                  21
Baroin-Tourancheau, Anne                                            22
Ruger Herreros, Carmen                                              23
Damaj, Raghida                                                      24
Serrano, Aurelio                                                  25-26
Siddiqui, Ruqaiyyah                                                 27
Aubusson-Fleury, Anne                                               28
Bricheux, Geneviève                                                 29
Calvo, Purificación                                                 30
Gallego, Andrea                                                     31
Palomino Gutiérrez, Carmen                                          32
SESSION III. ECOLOGY AND BIOTECHNOLOGY OF FREE-LIVING PROTISTS
Conferences on the Role of Protists on Wastewater Treatment and
Reuse. Chairperson: Juan Carlos Gutiérrez
López García, Purificación                                          34
Pereira do Amaral, António Luís                                     35




                                    6
FSFCP, Sevilla '08




                       INDEX OF ABSTRACTS BY AUTHORS
Arregui, Lucía                                                        36
Amaro, Francisco                                                      37
Fajon, Céline                                                         38
Aguilar, María                                                        39
Canals, Oriol                                                         40
Chatzinotas, Antonis                                                  41
Conty, Ana                                                            42
Serrano, Susana                                                       43
SESSION IV. BIOMEDICINE AND BIOTECHNOLOGY          OF   PARASITIC
PROTISTS. Chairperson: Federico Valverde
Khan, Naveed Ahmed                                                  45-46
Guillen, Nancy                                                        47
Siddiqui, Ruqaiyyah                                                   48
Moktar, Norhayati                                                     49
Gestal, Camino                                                        50
Maíllo, Pedro Andrés                                                51-52
Umar, Nor Aini                                                        53
SESSION V. PROTIST EVOLUTION. Chairperson: Loïc Morin
Lecroq, Béatrice                                                      55
Albi Rodríguez, Tomás                                                 56
Lara, Enrique                                                         57
Valverde, Federico                                                    58
Amar, Laurance                                                        59
Hernández-Piñero, Sara                                                60
Lecroq, Béatrice                                                      61
Marande, William                                                      62




                                    7
FSFCP, Sevilla '08




Opening Lecture




       8
Session III, Oral                                   FSFCP, Sevilla '08

  Microscopic characterization of activated sludge flocs from
  full-scale plants with advanced biological nitrogen removal
  systems

  Lucía Arregui, Pérez-Uz B, Calvo P, Salvadó H, Fernández N,
  Rodriguez E, Zornoza A and Serrano S. Departamento de
  Microbiología III, Universidad Complutense de Madrid.
  arregui@bio.ucm.es

  Protozoa and metazoan are considered good indicators of the
  treatment quality in activated sludge systems being
  sensitive to physical, chemical and operational processes.
  Therefore, it is possible to correlate the predominance of
  certain species or groups and operational parameters of the
  plant. This work presents a semi-automatic image analysis
  procedure for the recognition of the metazoan and protozoa
  species most frequently found in WWTP by determining
  morphological   data    and    multivariable    analysis   with
  discriminant analysis and neural network techniques. The
  non-stalked organisms' identification obtained individual
  recognition percentages above 80% with insignificant
  misclassification errors (usually bellow 1%) therefore
  yielding good overall recognition performances (above 80%).
  Furthermore,    a    closer     look    revealed    a    slight
  misclassification problem between the Monogononta and
  Digononta assessment. Although the overall results were not
  satisfactory for the stalked organisms, the identification
  ability of the biological indicators Opercularia and V.
  microstoma has attained some degree of confidence in
  accurately    establishing     their    presence    on    WWTP.
  Furthermore, good identification percentages were obtained
  for the most significant Carnivorous and large stalk
  Colonial whilst the less significant Vorticella and small
  stalk Colonial only attained reasonable identification
  percentages.   The   main    protozoa    and   metazoa   groups
  (flagellates, ciliates, sarcodines, and metazoa) and
  protozoan   ciliates    (carnivorous,     crawling,   swimming,
  sessile and non-ciliated) recognition attained good overall
  recognition performances (above 95%, except for crawling
  ciliates),   with   global    protozoa   and   metazoa   groups
  assessment around 95%. The overall recognition performance
  for the plant operating conditions assessment were quite
  fair for the effluent quality, aeration and nitrification
  evaluation (above 80%) and good on the sludge age
  determination (91.7%). Close related to the overall
  results, the assessment of critical conditions such as low
  effluent quality, low aeration, and fresh sludge attained
  also good overall recognition performance levels around
  90%. From these results it may be inferred that the current
  methodology can be used to predict at some extent, critical
  WWTP conditions in a feasible time period (within few
  hours) and without the need of specialized personnel in
  protozoology. The acquisition of the protozoa and metazoa
  images can be performed in a one to two hours period for a
  total of one to two hundred images, whilst the images
  treatment, data determination and analysis in two to three
  hours.



                                36

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2008 - Microscopic characterization of activated sludge flocs from full scale plants with advanced biological nitrogen removal

  • 1.
  • 2. FSFCP, Sevilla '08 Initial Presentation of the Congress Dear Protistologist, On behalf of “Grupo Especializado de Protistología” and “Groupment de Protistologues de Langue Française”, we have the pleasure to announce you the conjoint celebration in 2008 of their respective meetings. The gathering, called First Spanish-French Congress on Protistology, will be held in Seville, from 4th to 6th June. The aim of this meeting is to promote Protistology in Europe through scientific exchange. Official language is English. We shall make up an attractive scientific program and encourage the active participation of young researchers (pre-, post-docs); the city will provide participants with nice spring weather, history, and charm. The scientific program will be divided in three main axes, Evolution of protists, Biology of free-living protists, and Biology of parasitic protists, with oral presentations and posters. Topics should be set up according to received abstracts. If financial support allows it, two granted talks, opening and closing the meeting, each given by an outstanding protistologue during 60 minutes, are foreseen. Oral communications will be held in morning and afternoon sessions. Every session will include one talk, given by a senior researcher during 45 minutes, five secondary talks, given by young researchers during 20 minutes. All submitted abstracts should be considered as potential for oral presentations; selection will be carried out by the scientific committee of the meeting. Non-selected abstracts should be presented as posters. A session for poster discussion will be included also. 2
  • 3. FSFCP, Sevilla '08 Organizing Committee Grupo Especializado de Protistología (GEP), Sociedad Española de Microbiología (SEM). Groupement des Protistologues de Langue Française (GPLF). The Federation of European Protistological Societies (FEPS), a non-profit association of national and cross-national European Societies of Protistology and less formal national groups of Protistology of diverse European countries, supports the meeting President: Eduardo Villalobo. Departamento de Microbiología, Universidad de Sevilla. SPAIN. GEP-SEM. Vice-Presidents: Aurelio Serrano. Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC, Sevilla. SPAIN. GEP-SEM. Isabelle Desportes Museum National d'Histoire Naturelle, Paris. FRANCE. GPLF. Scientific Committee -Dr. Antonio Torres, Universidad de Sevilla, Sevilla. SPAIN. -Dr Gabriel Gutiérrez, Universidad de Sevilla, Sevilla. SPAIN. -Dr. Federico Valverde, Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC, Sevilla. SPAIN. -Dr. Susana Serrano, Universidad Complutense de Madrid, Madrid. SPAIN. -Dr. Juan Carlos Gutiérrez, Universidad Complutense de Madrid, Madrid. SPAIN. -Dr. Luis Miguel Ruiz Pérez, Instituto de Parasitología y Biomedicina López- Neyra, CSIC, Granada. SPAIN. -Dr. Loïc Morin, Université de Paris Sud, Orsay. FRANCE. -Dr. Anne Baroin-Tourancheau, Université de Paris Sud, CNRS, Orsay. FRANCE. -Dr. Purificación López García, Université de Paris Sud, CNRS, Orsay. FRANCE. -Dr. Nancy Guillén, Institut Pasteur, CNRS, Paris. FRANCE. 3
  • 4. FSFCP, Sevilla '08 Abbreviated Scientific Program Wednesday, 4th June -12:00-14:00 h. Registration. -15:00-15:15 h. Welcome talk by Dr. Eduardo Villalobo (President of the Congress), Dr. Loïc Morin (President of GPLF), and Dr. Aurelio Serrano (President of GEP-SEM). -15:15-16:15 h. Opening lecture by Dr. Andrés Aguilera. AFTERNOON, SESSION I. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF PARASITIC PROTISTS. Chairperson: Dr. Luis Miguel Ruiz Pérez -16:30-18:00 h. Oral presentations and discussions. -18:00-18:30 h. Poster pasting. -20:00-22:00 h. Welcome cocktail at Patio del Rectorado, Universidad de Sevilla. Thursday, 5th June. MORNING, SESSION II. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF FREE- LIVING PROTISTS. Chairperson: Dr. Geneviéve Bricheux -09:00-10:00 h. Main talk by Dr Philippe Bouchard. -10:00-10:30 h. Coffee break. -10:30-12:30 h. Oral presentations and discussions. -12:30-13:30 h. Posters discussion session. I Jornadas Internacionales de Tratamiento y Reutilización de Aguas Residuales: El Papel de los Protistas (only in spanish). Dr. Susana Serrano and Dr. Blanca Pérez Uz. Moderadora: Pilar Calvo -13:30-15:00 h. Lunch and coffee. AFTERNOON, SESSION III. ECOLOGY AND BIOTECHNOLOGY OF FREE-LIVING PROTISTS. Chairperson: Dr. Juan Carlos Gutiérrez -15:00-17:30 h. Conferences on the Role of Protists on Wastewater Treatment and Reuse. Dr. Purificación López García, Dr. António Luís Pereira do Amaral and Dr. Lucía Arregui. -17:30-19:00 h. Oral presentations and discussions. -20:30 h. “Tapas tour” in the city centre. Friday, 6th June MORNING, SESSION IV. BIOMEDICINE AND BIOTECHNOLOGY OF PARASITIC PROTISTS. Chairperson: Dr. Federico Valverde -09:00-10:00 h. Main talk by Dr. Naveed Ahmed Khan. -10:00-10:30 h. Coffee break. -10:30-12:00 h. Oral presentations and discussions. -12:00-13:30 h. Posters discussion session. -13:30-15:00 h. Lunch and coffee. AFTERNOON, SESSION V. PROTIST EVOLUTION. Chairperson: Dr. Loïc Morin -15:00-15:15. Closing talk by Dr. Ernesto García López (Vice-president Sociedad Española de Microbiología) -15:15-16:00 h. Main talk by Béatrice Lecroq. -16:00-18:00 h. Oral presentations and discussions. -18:00-19:00 h. Closing lecture by Dr. David Moreira. -20:15-21:15 h Visit to Reales Alcázares. 4
  • 5. FSFCP, Sevilla '08 Congress Venue The Congress will be held in Seville (SW, Spain) at "ESCUELA TÉCNICA SUPERIOR DE INGENIERÍA INFORMÁTICA (ETSII), UNIVERSIDAD DE SEVILLA", situated in "Campus Reina Mercedes" (Avenida Reina Mercedes, 41012 Sevilla). We will meet in the "Salón de Actos" of the "Escuela", with capacity for more than 300 people, provided with audiovisual media, and located in the first floor of the building. 5
  • 6. FSFCP, Sevilla '08 INDEX OF ABSTRACTS BY AUTHORS OPENING LECTURE Aguilera, Andrés 9 CLOSING LECTURE Moreira, David 11 SESSION I. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF PARASITIC PROTISTS. Chairperson: Luis Miguel Ruiz Pérez Kohl, Linda 13 López Farfán, Diana 14 Aranguren, Raquel 15 Arroyo, Francisco T. 16 Marques, Adam 17 Marques, Adam 18 Weber, Christian 19 SESSION II. MOLECULAR/CELLULAR BIOLOGY, AND PHYSIOLOGY OF FREE-LIVING PROTISTS. Chairperson: Geneviève Bricheux Bouchard, Philippe 21 Baroin-Tourancheau, Anne 22 Ruger Herreros, Carmen 23 Damaj, Raghida 24 Serrano, Aurelio 25-26 Siddiqui, Ruqaiyyah 27 Aubusson-Fleury, Anne 28 Bricheux, Geneviève 29 Calvo, Purificación 30 Gallego, Andrea 31 Palomino Gutiérrez, Carmen 32 SESSION III. ECOLOGY AND BIOTECHNOLOGY OF FREE-LIVING PROTISTS Conferences on the Role of Protists on Wastewater Treatment and Reuse. Chairperson: Juan Carlos Gutiérrez López García, Purificación 34 Pereira do Amaral, António Luís 35 6
  • 7. FSFCP, Sevilla '08 INDEX OF ABSTRACTS BY AUTHORS Arregui, Lucía 36 Amaro, Francisco 37 Fajon, Céline 38 Aguilar, María 39 Canals, Oriol 40 Chatzinotas, Antonis 41 Conty, Ana 42 Serrano, Susana 43 SESSION IV. BIOMEDICINE AND BIOTECHNOLOGY OF PARASITIC PROTISTS. Chairperson: Federico Valverde Khan, Naveed Ahmed 45-46 Guillen, Nancy 47 Siddiqui, Ruqaiyyah 48 Moktar, Norhayati 49 Gestal, Camino 50 Maíllo, Pedro Andrés 51-52 Umar, Nor Aini 53 SESSION V. PROTIST EVOLUTION. Chairperson: Loïc Morin Lecroq, Béatrice 55 Albi Rodríguez, Tomás 56 Lara, Enrique 57 Valverde, Federico 58 Amar, Laurance 59 Hernández-Piñero, Sara 60 Lecroq, Béatrice 61 Marande, William 62 7
  • 9. Session III, Oral FSFCP, Sevilla '08 Microscopic characterization of activated sludge flocs from full-scale plants with advanced biological nitrogen removal systems Lucía Arregui, Pérez-Uz B, Calvo P, Salvadó H, Fernández N, Rodriguez E, Zornoza A and Serrano S. Departamento de Microbiología III, Universidad Complutense de Madrid. arregui@bio.ucm.es Protozoa and metazoan are considered good indicators of the treatment quality in activated sludge systems being sensitive to physical, chemical and operational processes. Therefore, it is possible to correlate the predominance of certain species or groups and operational parameters of the plant. This work presents a semi-automatic image analysis procedure for the recognition of the metazoan and protozoa species most frequently found in WWTP by determining morphological data and multivariable analysis with discriminant analysis and neural network techniques. The non-stalked organisms' identification obtained individual recognition percentages above 80% with insignificant misclassification errors (usually bellow 1%) therefore yielding good overall recognition performances (above 80%). Furthermore, a closer look revealed a slight misclassification problem between the Monogononta and Digononta assessment. Although the overall results were not satisfactory for the stalked organisms, the identification ability of the biological indicators Opercularia and V. microstoma has attained some degree of confidence in accurately establishing their presence on WWTP. Furthermore, good identification percentages were obtained for the most significant Carnivorous and large stalk Colonial whilst the less significant Vorticella and small stalk Colonial only attained reasonable identification percentages. The main protozoa and metazoa groups (flagellates, ciliates, sarcodines, and metazoa) and protozoan ciliates (carnivorous, crawling, swimming, sessile and non-ciliated) recognition attained good overall recognition performances (above 95%, except for crawling ciliates), with global protozoa and metazoa groups assessment around 95%. The overall recognition performance for the plant operating conditions assessment were quite fair for the effluent quality, aeration and nitrification evaluation (above 80%) and good on the sludge age determination (91.7%). Close related to the overall results, the assessment of critical conditions such as low effluent quality, low aeration, and fresh sludge attained also good overall recognition performance levels around 90%. From these results it may be inferred that the current methodology can be used to predict at some extent, critical WWTP conditions in a feasible time period (within few hours) and without the need of specialized personnel in protozoology. The acquisition of the protozoa and metazoa images can be performed in a one to two hours period for a total of one to two hundred images, whilst the images treatment, data determination and analysis in two to three hours. 36