Electron micrograph ultrastructure_magnification

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Lesson notes for explaining how to calculate magnification and specimen size

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Electron micrograph ultrastructure_magnification

  1. 1. Scanning electron microscope image of a snout beetlehttp://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012
  2. 2. Calculating Linear Magnification of an Electron-Micrograph using its scale barThere are three steps:Measure the length of the scale bar - in mm.Convert your measurement into µm. 32mmCalculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS) 32 x 1000 = 32000µmMagnifiation = the number of times bigger the scale bar actually is, compared to themeasurement written on it? 32000µm / 100 µm = 320x magnification
  3. 3. Scanning electron microscope image of a snout beetlehttp://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012
  4. 4. Calculating Specimen Size using a scale barThere are four steps:Measure the length of the Specimen in mm.(show working)Measure the length of the scale bar in mm.Calculate how many scale bar lengths make the specimen.(Divide length of specimen by length of scale bar)Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar) 2.6 x 100 µm = 260µm
  5. 5. Scanning electron microscope image of a snout beetle http://remf.dartmouth.edu/images/insectPart2SEM/source/20.html Image: public domain. Feb. 2012Magnifiation = x 320
  6. 6. Calculating Specimen Size using magnificationThere are three steps:Measure the length of the Image of the Specimen in mm.(show working)Convert the length of the Specimen image to µm.(x 1000)Calculate the actual size.Divide the length of the specimen image by the magnification(UNITS are µm)
  7. 7. Electron microscope image of pollen grains from oriental lily.http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012
  8. 8. Calculating Linear Magnification of an Electron-Micrograph using its scale barThere are three steps:Measure the length of the scale bar - in mm.Convert your measurement into µm.Calculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS)Magnifiation = the number of times bigger the scale bar actually is, compared to themeasurement written on it? magnification
  9. 9. Electron microscope image of pollen grains from oriental lily.http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012
  10. 10. Calculating Specimen Size using a scale barThere are four steps:Measure the length of the Specimen in mm.(show working)Measure the length of the scale bar in mm.Calculate how many scale bar lengths make the specimen.(Divide length of specimen by length of scale bar)Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar)
  11. 11. Electron microscope image of pollen grains from oriental lily. http://remf.dartmouth.edu/images/botanicalPollenSEM/source/3.html Image: public domain. Feb. 2012Magnification = x 2500
  12. 12. Calculating Specimen Size using magnificationThere are three steps:Measure the length of the Image of the Specimen in mm.(show working)Convert the length of the Specimen image to µm.(x 1000)Calculate the actual size.Divide the length of the specimen image by the magnification(UNITS are µm)
  13. 13. Human leukocyte - showing golgi apparatushttp://remf.dartmouth.edu/images/humanBloodCellsTEM/source/3.html Image: public domain. Feb. 2012
  14. 14. Calculating Linear Magnification of an Electron-Micrograph using its scale barThere are three steps:Measure the length of the scale bar - in mm.Convert your measurement into nm this time.Calculate the magnification.(Magnification = measured length /the number written.As long as you have the SAME UNITS)Magnifiation = the number of times bigger the scale bar actually is, compared to themeasurement written on it? magnification
  15. 15. Calculating Specimen Size using a scale barThere are four steps:Measure the length of the Specimen in mm.(show working)Measure the length of the scale bar in mm.Calculate how many scale bar lengths make the specimen.(Divide length of specimen by length of scale bar)Calculate the size. Multiply the scale bar label by the last answer.(UNITS are the same as the scale bar)

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