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Mutations rII1 and rII2 are known to fall into the rIIA and rIIB cistrons (genes) respectively.
Mutations rII1 and rII2 are used to infect E. coli B at very low multiplicity of infection (m.o.i.)
and the progeny phage collected. These phage are then used to infect strain K at very low m.o.i..
The resulting phage are diluted and plated on both E. coli B and E. coli K. Do you expect more
plaques on strain B, on Strain K, an equal amount on both or is it impossible to tell? Explain
Solution
Strain B have more plaques because it supports the growth of all viruses,wild type T4 as well as
rII mutants which are able to complete their lifecycle in this strain thus giving total number of
viruses developed.
Strain K have low number of plaques because in this strain only wild type T4viruses can grow
and complete their lifecycle.

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Mutations rII1 and rII2 are known to fall into the rIIA and rIIB cis.pdf

  • 1. Mutations rII1 and rII2 are known to fall into the rIIA and rIIB cistrons (genes) respectively. Mutations rII1 and rII2 are used to infect E. coli B at very low multiplicity of infection (m.o.i.) and the progeny phage collected. These phage are then used to infect strain K at very low m.o.i.. The resulting phage are diluted and plated on both E. coli B and E. coli K. Do you expect more plaques on strain B, on Strain K, an equal amount on both or is it impossible to tell? Explain Solution Strain B have more plaques because it supports the growth of all viruses,wild type T4 as well as rII mutants which are able to complete their lifecycle in this strain thus giving total number of viruses developed. Strain K have low number of plaques because in this strain only wild type T4viruses can grow and complete their lifecycle.