1. HW#2 Genetics 320, Fall 07. Due Wed, Sept 12 The rules for this homework are the same: type any answers that involve words, except if you are filling in a table then write CLEARLY, or it will be marked wrong. If you don’t know what CLEARLY means, type your answers in the table. I give you a lot more problems this week than last. Many questions are revised from previous exam questions, to give you an idea what exam questions might look like.
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5. 4. Consider the cell 1 and cell 2 with the chromosomes shown. In Cell 1, Gene A is 50cM from the centromere, and in Cell 2 Gene C is 10cM from the centromere. In both cells the wildtype allele is on the top homolog, and the mutant allele on the bottom homolog. This problem involves mitotic recombination . Gene A+ + - a. Will Cell 1 or Cell2 form homozyous mutants at the highest frequency? Explain. b. A new Cell 3 is like Cell 1 except that it has a recessive lethal mutation. d- 10cM to the left of the GeneA+ allele. Where would the D+ allele be in Cell 3? (this should be obvious) c. Will Cell 1 or Cell 3 give the most viable a-/a- mutants? Explain. Cell 1 Cell 2 Gene C + -
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7. 6. Explain why a person with a germline genotype of p53+/- is prone to cancer while a person with a germline genotype of CFRT-/+ is not prone to cystic fibrosis. 7. In the pedigree shown below, compare the relative extent of heterology (between any two homologous chromosome) in individual 1, individual 2 and individual 3. 1 2 3
![Page 1 ,[object Object],[object Object],p53 binding site gcgTATAccgctacgtagg c tttATGCGATTTAAACCCTAGcccgcaag +1 +5 Transcribed? Translated (#amino acids) Functional? a. p53+/+ YES YES (full length) YES b. TATAcc to TggAcc c. Delete c at +1 d. Insert T between 7G &8C e. Delete ccc after TAG f. AAA to AAG Enter YES or NO. 1pt each. Enter # of amino acids if different from wildtype gene. You can explain ambiguities on the other side of this sheet (you shouldn’t need to!) g. Explain briefly your answer to c and 2d.](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)