In Drosophila the gene Tra encodes a protein that is expressed only in females. Individuals homozygous for this mutation be male but they are sterile.this is mainly because In males, the Sex lethal splice factor is not made; consequently, the Transformer message is not properly processed, and Transformer proteins are not produced. This causes an alternative splicing of Doublesex mRNA to an mRNA that codes for a male version of Doublesex protein. Solution In Drosophila the gene Tra encodes a protein that is expressed only in females. Individuals homozygous for this mutation be male but they are sterile.this is mainly because In males, the Sex lethal splice factor is not made; consequently, the Transformer message is not properly processed, and Transformer proteins are not produced. This causes an alternative splicing of Doublesex mRNA to an mRNA that codes for a male version of Doublesex protein..