1. Tyrosinase is involved in the synthesis of Melanin pigment in mammalian cells by using tyrosine as a substrate. In general phenolic compounds are best substrates for tyrosinase as it is responsible for browning of fruits and cut vegetables. 2. No, tyrosinase does not exhibit high level of specificity because it has broad substrate specificity for phenolic compounds. 3. Tyrosinase exhibit group specificity that is the presence of functional group which is phenol group. 4. Inhibition can be competitive or non-competitive. Competitive inhibitors are substrate analogs that is it resemble the substrate. Non-competitive inhibitors bind to the region other than active site for substrate binding. Benzoic acid is the substrate analog so it is competitive inhibitor. Phenylthiourea and resorcinol is a non-competitive inhibitor. 6. 0 C is freezing temperature and 70 C is denaturing temperature for enzyme. Enzyme reaction is best at the optimum temperature or physiological temperature. Solution 1. Tyrosinase is involved in the synthesis of Melanin pigment in mammalian cells by using tyrosine as a substrate. In general phenolic compounds are best substrates for tyrosinase as it is responsible for browning of fruits and cut vegetables. 2. No, tyrosinase does not exhibit high level of specificity because it has broad substrate specificity for phenolic compounds. 3. Tyrosinase exhibit group specificity that is the presence of functional group which is phenol group. 4. Inhibition can be competitive or non-competitive. Competitive inhibitors are substrate analogs that is it resemble the substrate. Non-competitive inhibitors bind to the region other than active site for substrate binding. Benzoic acid is the substrate analog so it is competitive inhibitor. Phenylthiourea and resorcinol is a non-competitive inhibitor. 6. 0 C is freezing temperature and 70 C is denaturing temperature for enzyme. Enzyme reaction is best at the optimum temperature or physiological temperature..