10. The push button is pushed to the second stop when we pipette out or transfer the sample, and to completely void the sample without leaving any sample in the pipette tips 11. The button is to be released after it is withdrawn from the solution/liquid. If it is released prior to withdrawal, the solution reenters the pipette tip. 12. If the push button is pressed to the second stop and is released even before the pipette tip is withdrawn from the solution, the solution enters the pipette. 13. When concentrations or dilutions of the sample that are to be transferred changes, when different solutions or chemicals are transferred, when containment is required, tips need to be changed with each transfer. 14. The fragments obtained will have blunt ends if the restriction enzyme cuts at the center. Yes. They are still useful because linkers or adapters can be attached to the blunt end to make them useful. Moreover, new restriction sites can be created by attaching linkers or adapters. 15. Yes it can be done. The restriction enzyme creates the same overhanging ends since it recognizes the same restriction site in both the DNA. Therefore, the two different DNA can basepair at the sticky ends. Solution 10. The push button is pushed to the second stop when we pipette out or transfer the sample, and to completely void the sample without leaving any sample in the pipette tips 11. The button is to be released after it is withdrawn from the solution/liquid. If it is released prior to withdrawal, the solution reenters the pipette tip. 12. If the push button is pressed to the second stop and is released even before the pipette tip is withdrawn from the solution, the solution enters the pipette. 13. When concentrations or dilutions of the sample that are to be transferred changes, when different solutions or chemicals are transferred, when containment is required, tips need to be changed with each transfer. 14. The fragments obtained will have blunt ends if the restriction enzyme cuts at the center. Yes. They are still useful because linkers or adapters can be attached to the blunt end to make them useful. Moreover, new restriction sites can be created by attaching linkers or adapters. 15. Yes it can be done. The restriction enzyme creates the same overhanging ends since it recognizes the same restriction site in both the DNA. Therefore, the two different DNA can basepair at the sticky ends..