Biochemistry & Molecular Biology

1. Presentation on Biochemistry & Molecular Biology
MD.AMINUL ISLAM
Department of Pharmacy
World University of Bangladesh

2. Presentation Topic On
Molecular Characterization of Mungbean
Yellow Mosaic Disease and Coat Protein
Gene in Mungbean Varieties of
Bangladesh.

3. Materials and Methods
To design primers, MYMV coat protein gene
sequences were downloaded from NCBI
nucleotide data base (Morinaga et al.
Molecular Characterization of Mungbean
Yellow Mosaic Disease.

4. Materials and Methods
After aligning the sequences two regions with
maximum sequence homology were selected to
design forward (MYMVcpFwd: 5́ ACA CGA
GCT CCT CTA CCC CGA TAT CGAATG 3́)
For the identification of MYMV infection in the
collected samples, isolated DNA samples were
subjected to PCR using CP specific primers
following an initial denaturation at 95ºC for 5
min.

5. Materials and Methods
And 30 cycles of denaturation at 94ºC for 1
min annealing at 58ºC for 1 min.
Extension at 72ºC for 30 sec and reaction was
completed with final extension at 72ºC for 5
min.

6. Thank You……