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A generalized transducing phage is used to infect wildtype prototrophic E. coli, and the lysate is
used to transduce an abcderecipient. The recipient cells are plated on minimal medium supplied
with the indicated compounds. The results are shown in the following table. (Note that aindicates
a requirement for compound A, etc.) What can you conclude about the order of these genes?
Three of the genes are closely linked and have the order bdc, while genes a and e are not closely
linked to any other genes. The gene order of four of the genes is cead, but the placement of b is
unknown. Genes b,c and d are linked, but their order cannot be determined. Genes a and e are not
closely linked to any of the other genes. Genes a,c,d and e are closely linked, but their order
cannot be determined with certainty, while gene b is not closely linked to this group.

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E. coli gene order determined by transduction experiment

  • 1. A generalized transducing phage is used to infect wildtype prototrophic E. coli, and the lysate is used to transduce an abcderecipient. The recipient cells are plated on minimal medium supplied with the indicated compounds. The results are shown in the following table. (Note that aindicates a requirement for compound A, etc.) What can you conclude about the order of these genes? Three of the genes are closely linked and have the order bdc, while genes a and e are not closely linked to any other genes. The gene order of four of the genes is cead, but the placement of b is unknown. Genes b,c and d are linked, but their order cannot be determined. Genes a and e are not closely linked to any of the other genes. Genes a,c,d and e are closely linked, but their order cannot be determined with certainty, while gene b is not closely linked to this group.