You performed a Sanger sequencing reaction and obtained the following.pdf

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You performed a Sanger sequencing reaction and obtained the following result. The height of the peaks is unimportant. The 5 ' end of the sequence is at the left of the trace. The first blue peak (the tourth peak from the left end) represents a DNA fragment containing regular deoxynucleotide(s) or diNTP(s) (excluding the primer sequence). This tragment runs than the frament producing the first red peak (the fith peak from the left end through the gel during electrophoresis..

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