A) Mix substrate and buffer in Spectonic, 20 tubes as indicated below in Table 2. (DON'T add
AP yet - you will start each reaction one at a time by adding AP) Table 2. Prepare the 5 tubes as
follows and monitor each reaction using Spectropic 20. *"WAIT to add enzyme until you are
ready to start/monitor that specific reaction (see instructions below) Substratel = p-nitrophenyl
phosphate: 40kg/ml(Mr of pNPP=371.14g/mol) Buffer 2=0.1 M Tris-HCl, pH 8.0 Alkaline
phosphatase 3=3.3g/ml in TRIS buffer.
A) Mix substrate and buffer in Spectonic, 20 tubes as indicated below.pdf
1. A) Mix substrate and buffer in Spectonic, 20 tubes as indicated below in Table 2. (DON'T add
AP yet - you will start each reaction one at a time by adding AP) Table 2. Prepare the 5 tubes as
follows and monitor each reaction using Spectropic 20. *"WAIT to add enzyme until you are
ready to start/monitor that specific reaction (see instructions below) Substratel = p-nitrophenyl
phosphate: 40kg/ml(Mr of pNPP=371.14g/mol) Buffer 2=0.1 M Tris-HCl, pH 8.0 Alkaline
phosphatase 3=3.3g/ml in TRIS buffer
