5) True or false: (4) Fermentation is used by organisms who typically perform anaerobic respiration. Like aerobic respiration, fermentation takes place in the mitochondria of cells. Organisms that utilize fermentation still perform glycolysis in the absence of O 2 . Fermentation is used only by single-celled organisms. 6) Why are methods of anaerobic respiration, such as fermentation, less efficient than aerobic respiration? (1).