1. MOHAMAD RAAFI B AHMAD
TAIB
152036
(Practical Report Presentation)

3. PRINCIPAL
Dry (Bomb) analysis
 Instrument use Elemental Analyzer (EA)
 For nitrogen
Wet analysis (solubility)
 Organic material need to be converted to
inorganic material
 Ashing (greyish-white)
 Instrument use furnace and ICP
 The elements contain in the leaves can be
diluted using dilute acid.

4. Methodology
(MS 667:Pt. I-VIII:1980)

5. ASHING AND PREPARATION OF
ASH SOLUTION
 Leaf need to be dry and grind to get homogenous.
 Weigh about 1 to 2 g of the sample into a silica
dish.
 Place the dish in the furnace (500⁰C for 5 hours)
until greyish-white

6.  Add 2 ml Hydrochloric acid and evaporate to dryness
on a hot plate.
 Add 10 ml of nitric acid and digest on a water-bath
for 1 hour.

7.  Filter into a 100 ml volumetric flask using filter
paper.
 Make up to volume with water and mix well.

9. PRINCIPAL
Wet analysis (solubility)
 Inorganic material.
 Use spectrometer uv to get the wavelength of
silica (yellow colour).

10.  Weight sample 0.1g ,put in 250 ml
Erlenmeyer flask
 Add 5g Sodium carbonate
 Add 100ml distilled water
 Boil on Hot plate for 15-30 min
ANALYSIS OF SILICA

11.  Pour 5 ml sulfuric acid
 Boil in water bath (6 hours), leave 2 days
 Filter samples & dilute 10 times
 Pipette 10 ml sample & add molybdate, acid
reagent and citric acid (shake)
 Obtain result from spectrophotometer
(410 nm yellow wavelength)