DNA sequencing is a technique that provides a detailed analysis of the structure of DNA and consists of a set of techniques and biochemical methods that allow us to determine the sequence of nucleotides (A, C, G, and T) analysis is DNA.
In the mid-1970s happened a revolution in technology for identifying DNA sequence. In 1977 was published the complete nucleotide sequence of a viral genome (φ X174, 5375 nucleotides long). This milestone in molecular biology occurred in the laboratory of Frederick Sanger, who identified the amino acid sequence of the polypeptide (insulin) 25 years earlier.
Bioinformatics is the application of computer technology to information in molecular biology, encompassing aspects of the acquisition, processing, distribution, analysis, interpretation and integration of biological information. There are several databases that organize information and they are often used, which are presented in the following bioinformatics centers: GenBank (NCBI) and BOLD Systems
The NCBI database (established in 1988) has a public database, with three components. Creating databases (store biological data), development of algorithms and statistics to determine relationships between databases, and use these tools to analyze and interpret various types of biological data (sequences of DNA, RNA, protein, protein structure, gene expression, biochemical pathways)
The Barcode of Life Data Systems (BOLD) is an informatics workbench aiding the acquisition, storage, analysis, and publication of DNA barcode records. By assembling molecular, morphological, and distributional data, it bridges a traditional bioinformatics chasm. BOLD is freely available to any researcher with interests in DNA barcoding. By providing specialized services, it aids the assembly of records that meet the standards needed to gain BARCODE designation in the global sequence databases. Because of its web-based delivery and flexible data security model, it is also well positioned to support projects that involve broad research alliances.
1. BIOINFORMATICS ANALYSIS OF
NUCLEOTIDE SEQUENCES
Members:
• Avellaneda Vergara Adrian Gustavo
• Yarasca Cerna Withney Aracely
• Zegarra Aguinaga Janeth Alexandra
• Farfan Hernandez Kevin Jhonny
• Graham Angeles Laura Andrea
2. INTRODUCTION
• DNA sequencing is a technique that provides a detailed analysis of the structure of DNA and
consists of a set of techniques and biochemical methods that allow us to determine the
sequence of nucleotides (A, C, G, and T) analysis is DNA.
• In the mid-1970s happened a revolution in technology for identifying DNA sequence. In
1977 was published the complete nucleotide sequence of a viral genome (φ X174, 5375
nucleotides long). This milestone in molecular biology occurred in the laboratory of
Frederick Sanger, who identified the amino acid sequence of the polypeptide (insulin) 25
years earlier.
• Bioinformatics is the application of computer technology to information in molecular
biology, encompassing aspects of the acquisition, processing, distribution, analysis,
interpretation and integration of biological information. There are several databases that
organize information and they are often used, which are presented in the following
bioinformatics centers: GenBank (NCBI) and BOLD Systems
3. • The NCBI database (established in 1988) has a public database, with three components.
Creating databases (store biological data), development of algorithms and statistics to
determine relationships between databases, and use these tools to analyze and interpret
various types of biological data (sequences of DNA, RNA, protein, protein structure, gene
expression, biochemical pathways)
• The Barcode of Life Data Systems (BOLD) is an informatics workbench aiding the
acquisition, storage, analysis, and publication of DNA barcode records. By assembling
molecular, morphological, and distributional data, it bridges a traditional bioinformatics
chasm. BOLD is freely available to any researcher with interests in DNA barcoding. By
providing specialized services, it aids the assembly of records that meet the standards
needed to gain BARCODE designation in the global sequence databases. Because of its web-based
delivery and flexible data security model, it is also well positioned to support projects
that involve broad research alliances.
57. STEP 27
Then we select, in this case 20, differet sequences that are similar
with our consensu sequence. We can see the similarity in the ítem
IDENT that is in red.