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HERBARIUM
TECHNIQUES &
FUNCTIONS
Dr. Manoj Joshi
Department of Botany
Herbarium
A collection of dried and
pressed plant arranged
according to a classification
system and available for
study or reference is known
as herbarium
( plural herbaria).
A name first applied by
linnaeus
-These specimens may be
whole plants or plant parts
and these will usually be in a
dried form, mounted on a
sheet.
• It is based on a scientific collection, in continuous
expansion of plant specimens which are carefully
dried, labelled and conveniently treated for its
permanent preservation.
• Its research work focuses on the collection of plant
specimens which are catalogued and stored
according to an orderly and systematic botanical
classification.
• They are also dried, preserved, identified, labelled
and computerized.
The project aim
- To study on various plants
- To contribute to scientific studies
- To preserve the catalogues of seeds and dried
herbarium plants
- To maintain plants on the edge of extinctions
- To inform the students and public about these
plants
- To inform them the effect of global warming on
ecological system
How to make a Herbarium?
1) Collecting the plants
2) Pressing and Drying
3) Mounting
1) Collecting the plants
• Choose good representatives of the plants
species
• Be careful that these plants must include root,
stem, flower and fruit
• Take notes and record by taking photos in the
field at the time of collection,
• Note these factors below:
“Date, collection number, location, habitat,
habit, special characteristics”
• Collect specimens in dry conditions, a
good time being mid-morning, after the
dew has dried but before the heat of the
day causes plants to wilt.
• If specimens are at all wet or you need to
wash soil off the roots then dry them
carefully before pressing.
• Use a pencil for these notes rather than a
pen because any damp/wetness can cause
ink to smudge and be unreadable
Materials for plant collecting:
• Plant press
• Plastic bags or nylon bag
• Garden secateurs & trowel
• Small note book & pencil
• Jeweller’s tags (optional)
• Camera (optional)
• GPS & altimeter (optional)
PRESSING AND DRYING
PRESSING & DRYING
- Plants must be clean before pressing
- They must also be put in a plastic bag or nylon bag, if it is hot they must be
watered to be fresh
- Place your plant between folded-out sheets of newspaper, although flimsy
or greaseproof paper is preferable for delicate material
- Arrange the plant carefully, trying to avoid overlapping.
- When you have finished arranging the specimens within the newspaper
sheets (or whatever combination of papers you have chosen), you then
need to intersperse them between corrugated card sheets to aid
ventilation.
- Finally place everything in your press and tighten well.
• For the first two to four days you will need to check daily and change the
blotting paper and/or other surrounding papers, and retighten the press, but
as the plants dry these checks can become less frequent.
• Warmth may be used to improve the drying rate, An oven set at 50°C may
be used but the heat must be no higher, otherwise the specimen will
become very brittle and damaged.
Materials for pressing
• Plant press
• Newspaper
• Greaseproof or flimsy paper for delicate structures
• Blotting paper
• Corrugated card
MOUNTING
• Cartridge paper for mounting
your specimens should preferably
be A3 size and acid-free; the
weight should be a minimum
180g/m2, and ideally with a
rough textured surface
• Using only one side of your
thick A3 cartridge paper,
arrange your specimens carefully,
making sure that they represent
the way the plant grows naturally
Example Herbarium Label
• Scientific name: Ranunulaceae, Ranunculus ficaria (family, genus and
species)
• Vernacular name(s): Lesser Celandine, Pilewort
• Collector’s name and specimen number: Lawrence 1
• Date of collection: 20th March 2003
• Locality: Orleans House Gardens, Twickenham, England
• Habitat: damp, clay soil, 20 yards from riverbank, growing in dappled shade
on the edge of deciduous woodland; nearby plant is Dock (Rumex
obtusifolius).
• Habit: perennial herb, up to 20 cm tall, with stems creeping and rooting
• Characteristics: leaves hairless glossy green, flowers bright glossy yellow,
turning white with age
MOUNTING AND STITCHING
The standard size of a herbarium sheet is
29 x42 cm. They are usually made of durable
card sheets. The dried specimens are glued
on herbarium sheets and the stem/branches
can be stitched/glued with cellophane tapes.
It is advisable to mount one specimen on
each herbarium sheet. Dissected & loose
parts, such as flowers, fruits & seeds, are
kept in paper packets & pasted to the
mounted sheet.
LABELLING
• Name of organization with which specimen plant
originated.
• Name of the family
• Botanical name of the plant
• Local name
• Locality of collection
• Date of collection
• Habitat of the plant
• Field notes & collection no.
• Name of collector
FILLING AND STORING
• Plant specimens, which have been properly mounted
& identified, are filled systematically in special
wooden/steel cabinets.
• The herbarium sheets loaded with specimens are filed
inside folders which are of various colour schemes
indicating species, genus, family, geographical area,
etc.
• Plants are arranged & stored following Bentham &
Hooker’s / Engler & Prantl’s system of classification.
• A periodical fumigation with chemicals & repoisoning
them by brushing with solution of HgCl2 & using insect
repellents would save the herbarium from damage &
check the loss of valuable plants.
HERBARIUMALMIRAH
ROLE OF HERBARIUM
• To act as a repository of dried plant specimens, safeguard them
against loss & destruction by fungi, insects, etc. & make them
available for study.
• Several herbaria of repute, keep Type Specimens-the principal proof
of the existence of a species, in safe custody, often in rooms with
restricted access.
• As original documents upon which knowledge of taxonomic characters
rests, herbarium specimens greatly help in developing floras, manuals
& monographs.
• Those engaged in taxonomic studies, can personally identify their
engaged collection by comparison with already identified herbarium
specimens.
• Voucher specimens preserved in various herbaria, provide an index of
specimens on which studies on chromosomes, phytochemistry,
ultrastructure micro-morphology, etc. have been undertaken.
• Most herbaria have specimens collected from different parts of the
world &, thus their scrutiny can provide information on the
geographical distribution of taxa.
herbarium-190813073311.pptx

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herbarium-190813073311.pptx

  • 1. HERBARIUM TECHNIQUES & FUNCTIONS Dr. Manoj Joshi Department of Botany
  • 2. Herbarium A collection of dried and pressed plant arranged according to a classification system and available for study or reference is known as herbarium ( plural herbaria). A name first applied by linnaeus -These specimens may be whole plants or plant parts and these will usually be in a dried form, mounted on a sheet.
  • 3. • It is based on a scientific collection, in continuous expansion of plant specimens which are carefully dried, labelled and conveniently treated for its permanent preservation. • Its research work focuses on the collection of plant specimens which are catalogued and stored according to an orderly and systematic botanical classification. • They are also dried, preserved, identified, labelled and computerized.
  • 4. The project aim - To study on various plants - To contribute to scientific studies - To preserve the catalogues of seeds and dried herbarium plants - To maintain plants on the edge of extinctions - To inform the students and public about these plants - To inform them the effect of global warming on ecological system
  • 5. How to make a Herbarium? 1) Collecting the plants 2) Pressing and Drying 3) Mounting
  • 6. 1) Collecting the plants • Choose good representatives of the plants species • Be careful that these plants must include root, stem, flower and fruit • Take notes and record by taking photos in the field at the time of collection, • Note these factors below: “Date, collection number, location, habitat, habit, special characteristics”
  • 7. • Collect specimens in dry conditions, a good time being mid-morning, after the dew has dried but before the heat of the day causes plants to wilt. • If specimens are at all wet or you need to wash soil off the roots then dry them carefully before pressing. • Use a pencil for these notes rather than a pen because any damp/wetness can cause ink to smudge and be unreadable
  • 8. Materials for plant collecting: • Plant press • Plastic bags or nylon bag • Garden secateurs & trowel • Small note book & pencil • Jeweller’s tags (optional) • Camera (optional) • GPS & altimeter (optional)
  • 10. PRESSING & DRYING - Plants must be clean before pressing - They must also be put in a plastic bag or nylon bag, if it is hot they must be watered to be fresh - Place your plant between folded-out sheets of newspaper, although flimsy or greaseproof paper is preferable for delicate material - Arrange the plant carefully, trying to avoid overlapping. - When you have finished arranging the specimens within the newspaper sheets (or whatever combination of papers you have chosen), you then need to intersperse them between corrugated card sheets to aid ventilation. - Finally place everything in your press and tighten well.
  • 11. • For the first two to four days you will need to check daily and change the blotting paper and/or other surrounding papers, and retighten the press, but as the plants dry these checks can become less frequent. • Warmth may be used to improve the drying rate, An oven set at 50°C may be used but the heat must be no higher, otherwise the specimen will become very brittle and damaged.
  • 12. Materials for pressing • Plant press • Newspaper • Greaseproof or flimsy paper for delicate structures • Blotting paper • Corrugated card
  • 13. MOUNTING • Cartridge paper for mounting your specimens should preferably be A3 size and acid-free; the weight should be a minimum 180g/m2, and ideally with a rough textured surface • Using only one side of your thick A3 cartridge paper, arrange your specimens carefully, making sure that they represent the way the plant grows naturally
  • 14. Example Herbarium Label • Scientific name: Ranunulaceae, Ranunculus ficaria (family, genus and species) • Vernacular name(s): Lesser Celandine, Pilewort • Collector’s name and specimen number: Lawrence 1 • Date of collection: 20th March 2003 • Locality: Orleans House Gardens, Twickenham, England • Habitat: damp, clay soil, 20 yards from riverbank, growing in dappled shade on the edge of deciduous woodland; nearby plant is Dock (Rumex obtusifolius). • Habit: perennial herb, up to 20 cm tall, with stems creeping and rooting • Characteristics: leaves hairless glossy green, flowers bright glossy yellow, turning white with age
  • 15. MOUNTING AND STITCHING The standard size of a herbarium sheet is 29 x42 cm. They are usually made of durable card sheets. The dried specimens are glued on herbarium sheets and the stem/branches can be stitched/glued with cellophane tapes. It is advisable to mount one specimen on each herbarium sheet. Dissected & loose parts, such as flowers, fruits & seeds, are kept in paper packets & pasted to the mounted sheet.
  • 16. LABELLING • Name of organization with which specimen plant originated. • Name of the family • Botanical name of the plant • Local name • Locality of collection • Date of collection • Habitat of the plant • Field notes & collection no. • Name of collector
  • 17. FILLING AND STORING • Plant specimens, which have been properly mounted & identified, are filled systematically in special wooden/steel cabinets. • The herbarium sheets loaded with specimens are filed inside folders which are of various colour schemes indicating species, genus, family, geographical area, etc. • Plants are arranged & stored following Bentham & Hooker’s / Engler & Prantl’s system of classification. • A periodical fumigation with chemicals & repoisoning them by brushing with solution of HgCl2 & using insect repellents would save the herbarium from damage & check the loss of valuable plants.
  • 19. ROLE OF HERBARIUM • To act as a repository of dried plant specimens, safeguard them against loss & destruction by fungi, insects, etc. & make them available for study. • Several herbaria of repute, keep Type Specimens-the principal proof of the existence of a species, in safe custody, often in rooms with restricted access. • As original documents upon which knowledge of taxonomic characters rests, herbarium specimens greatly help in developing floras, manuals & monographs. • Those engaged in taxonomic studies, can personally identify their engaged collection by comparison with already identified herbarium specimens. • Voucher specimens preserved in various herbaria, provide an index of specimens on which studies on chromosomes, phytochemistry, ultrastructure micro-morphology, etc. have been undertaken. • Most herbaria have specimens collected from different parts of the world &, thus their scrutiny can provide information on the geographical distribution of taxa.