Phytoplankton vs Zooplankton

1. bec©keTsciBa©wmécTwk
Manual for the propagation of
Moina macrocopa
eroberogeday³
bNÐit sIur:U hara: (Shiro Hara) nigelak rs; Narin
ERbsRmYlBIPasaGg;eKøseday³ elak G‘Uc Lg; nigelak sM NariT§
EksRmYleday³ naykdæanGPivDÆn_varIvb,kmμ énrdæ)alClpl
rkSasiTi§eday³ naykdæanGPivDÆn_varIvb,kmμ
FñÚ 2010

3. matikaGtßbT
i
GarmÖkfa>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-x-
esckþIEføgGMNrKuN>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-K-
1> lkçN³CIvsaRsþ>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-1-
2> RbPB nigrebobciBa©wmécTwk nigrukçCatibøg;tug>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-2-
2>1rebobciBa©wmrukçCatibøg;tugkøer:løa¬Chlorella sp.¦>>>>>>>>>>>>>>>>>>>>>>>-2-
2>2 rebobciBa©wmécTwk (Moina macrocopa)>>>>>>>>>>> >>>>>>>>>>>>> >>>>>>>>>>-3-
3> kareRbIR)as;CIkñúgkarciBa©wmrukçCatibøg;tug nigécTwk>>>>>>>>>>>>>>>>>>>>>>>>>>>>-4-
4> RbB½n§GagciBa©wmbøg;tug>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-4-
4>1 GagciBa©wmbøg;tugrukçCati¬køer:løa¦>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-5-
4>2 GagciBa©wmécTwk>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-8-
5> karRKb;RKg>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-8-
5>1karciBa©wmbøg;tugrukçCati¬Chlorella sp.¦ry³eBl4éf¶>>>>>>>>>>>>>>>-8-
5>2karciBa©wmécTwk (Moina macrocopa) ry³eBl4éf¶>>>>>>>>>>>>>>>>>>-9-
6> karRbmUlpl>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-9-
7> viFIsaRsþsMxan;²kñúgkarciBa©wmbøg;tug>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>-10-
-k-

4. GarmÖkfa
3
écTwkCaRbePTstVbøg;tugEdlrYmmaneRcInRbePTdUcCa r:UTIhV½r (Roti-
fer) m:UyNa (Moina sp.) nig kUBIb:Ud (Copepod)>>>. RbePTstVbøg;tugTaMgenH
CacMNIFmμCatid¾sMxan;sRmab;kUnRtIeTIbjas; eRBaHvasm,ÚreTAedaysarFatu
ciBa©wm nigkRmitRbUetGuInx<s;GacCYyeFVI[kUnRtImansuxPaBl¥ CYyCMrujkar
lUtlas;elOn nigGRtaKg;vgSx<s;. CaTUeTA enAkñúgRsHbMb:nkUnRtI stV
bøg;tugEtgEtlUtlas; nigekItkan;EteRcIneLIg kñúgGMLúgeBl 3 éf¶ bnÞab;BI
)andak;CIcUleTAkñúgRsHrYc. enARbeTséf nigevotNam )ancab;epþImdMeNIr
karciBa©wmécTwkRbePTenH cab;taMgBIqñaM 1990 mkemø:H.
GMLúgqñaM 2007 mCÄmNÐlRsavRCav nigGPivDÆn_varIvb,kmμTwksab
énrdæ)alClpl énRksYgksikmμ rukçaRbmaj; nigensaT EdlmanTItaMg
sßitenAkñúgextþéRBEvg k¾)ancab;epþImdMeNIrkarciBa©wmRbePTécTwkenH enAkñúg
RbB½n§GagsuIm:g;t¾ edaymankic©shkarCamYynwgPñak;garshRbtibtþikar
GnþrCatiCb:un (JICA). enAkñúgkarciBa©wmécTwkenH eyIg)aneFVIkarsikSaRsav
RCavbEnßmedayepþatCasMxan;eTAelIkareRbIR)as;CI nigviFIsaRsþciBa©wm.
eyIgsgÇwmfa Éksarbec©keTsciBa©wmécTwkRbePT Moina macroco-
pa enHnwgedIrtYnaTIy:agsMxan;enAkñúgkarCYyeFVI[RbesIreLIg kñúgdMeNIrplit
kmμkUnRtIBUC k¾dUcCakUnbgágBUCEdr edIm,IcUlrYmcMENkCMruj vis½yvarIvb,kmμ
enARBHraCaNacRkkm<úCa[kan;EtmankarrIkceRmInqab;rh½s.
-x-

5. esckþIEføgGMNrKuN
kk
eyIg´sUmEføgGMNrKuNy:agRCaleRCAcMeBaH Ék]tþmbNÐit
eNA FYk RbtiPUraCrdæaPi)alkm<úCa TTYlbnÞúkCaRbFanrdæ)alClpl én
RksYgksikmμ rukçaRbmaj; nigensaTEdl)anCYyeRCamERCgelIkTwkcitþkñúg
karsikSaRsavRCav[mandMeNIrkarl¥ nigTTYl)aneCaKC½y .
eyIg´ sUmEføgGMNrKuNpgEdr cMeBaHm®nþInaykdæanGPivDÆn_
varIvb,kmμ nigelak g:an; ehg RbFan nigelak sM NariT§ GnuRbFan
mCÄmNÐlRsavRCav nigGPivDÆn_varIvb,kmμTwksab rYmTaMgm®nþITaMgGs;Edl
)ancUlrYmkñúgskmμPaBkargarKeRmag nigkarsikSaRsavRCavenH RBmTaMg
elakSatoshi Chikami elakYoshitetsu Nukiyama RbFanTIRbwkSaKeRmag
begáInplitPaB nigpSBVpSayvarIvb,kmμTwksab (FAIEX Project/JICA) Edl
)andwknaMGnuvtþKeRmagRbkbedayPaBrlUn nigTTYl)aneCaKC½y.
ÉksarsþIBIbec©keTsciBa©wmécTwkenH RtUv)anerobcMcgRkgelcecj
CarUbrageLIg GaRs½yedaymankarCYy]btßmÖKaMRTEpñkhirBaØvtßúrbs;GgÁkar
écka (JICA).
éf¶TI 17 Ex FñÚ qñaM 2010
bNÐit s
sIIuurr::UU h
haarraa:: (Shiro Hara)
m®nþIbec©keTssμ½RKcitþCan;x<s;
GgÁkarécka (JICA)
-K-

7. 1> lkçN³CIvsaRsþ
écTwkRbePT m:UyNa ma:RkUkU)a: (Moina macrocopa) enHCaRbePT
stþvnþCati ¬BBYk kþam bgág¦manTMhMtUcsßitkñúgGm,Úr Cladocera nigRbePT
Daphnidae rs;enAtamtMbn;Twkrak; dUcCaenAkñúgRsH b¤ enAtamlUTwks¥úyEdl
CakaksMNl;TwkbeBa©jecalBITIRkug(rUbPaBTI 1¦. vaGacrs;)anenAkñúgTwk
s¥úybgáeLIgedaysarFatusrIragÁrlYyEdlmankRmitbrimaNGuksuIEhSn
rlayTab. cMNIrbs;écTwkenAkñúgFmμCatirYmmanBBYk)ak;etrI nigrukçCati
bøg;tugl¥it² EdleKehAfakøer:løa (Chlorella sp.) ¬rUbPaBTI 2¦.
écTwkCaRbePTcMNIrs;mankRmitsarFatuciBa©wmx<s;sRmab;kUnRtIeTIb
jas;. tamlT§plénkareFVIviPaK)anbgðaj[eXIjfa écTwkmanpÞúk
brimaNTwkRbmaN 94 PaKry nigRbUetGuInRbmaN 74 PaKry. vacUlcitþ
pøas;TIenAeBlyb;rhUtdl;RBwkRBlwm EdlCaGMLúgeBlmYymanbrimaN
GuksuIEhSnrlaykñúgTwkcuHdl;kRmitTab. bnÞab;mkeTot écTwk)anpøas;TI
bnþicmþg²mkRbmUlpþúMKñaenAelIépÞTwkCakEnøgEdlmanBnøWRKb;RKan; ehIyva
-1-
rrUUb
bP
PaaB
BT
TII1
1³³ ééc
cT
Twwk
keeB
Bj
jvv½½y
y rrUUb
bP
PaaB
BT
TII2
2³³ T
Twwk
kééb
bt
tg
g ¬
¬k
køøeerr::l
løøaa¦
¦

8. nwgpøas;TIRtLb;eTAEpñkxageRkamépÞTwkvij enAeBlsItuNðPaBTwkeLIg
ekþA. TMhMRbEvgbeNþaydgxøÜnécTwkmancab;BI 0/4 miløIEm:Rt eTA 1/6
miløIEm:Rt ehIyCaTUeTA jImanTMhMFMCageQμal rIÉdgxøÜnrbs;vavijmanBN’
Rkhm EtnwgERbeTACaRkhmRkemA enAeBlEdlkRmitbrimaNGuksuIEhSn
rlaykñúgTwkcuHdl;kRmitTab. cMeBaHécTwkeTIbjas;manTMhMCa mFümcab;BI
0/22 eTA 0/33 miløIEm:Rt dgxøÜnmanBN’RkhmRBElt b¤ RkhmesøkCag
écTwkFMeBjv½y.
eyIg)anGegáteXIjfa enAkñúgbnÞb;BiesaFn_EdlmanlkçxNÐsm
RsbenaHeFVI[écTwkjIGacbnþBUCBgkUn)anedayxøÜnÉg. écTwkmanvdþCIvit
rbs;vaCamFümry³eBl 7 éf¶ ehIyGacbnþBUCBgkUn)ankñúgry³eBl 7 éf¶
Cab;²KñaedaykñúgmYyéf¶GacBgkUn)an 20 k,al srub RbEhl 130 k,al
kñúgmYyCIvitrbs;va. pÞúyeTAvij krNIlkçxNÐminsmRsbGt;cMNIenaH
eFVI[écTwkjIminGacbnþBUCBgkUn)anedayxøÜnÉgeLIyenAeBlvaFMeBjv½y
luHRtaEtmaneQμaleTIbGacbnþBUC)anedayTMlak;Bg)ancMnYn 2 RKab;
kñúgjImYyk,al .
2> RbPB nigrebobciBa©wmécTwk nigrukçCatibøg;tug
2
2>>1
1 rreeb
boob
bc
ciiB
Baa©©wwm
mrruuk
kççC
Caat
tiib
bøøg
g;;t
tuug
g k
køøeerr::l
løøaa ¬Chlorella sp.¦
rukçCatibøg;tug køer:løa RtUv)aneKdYsvamkBIRsHEdleTIb)andak;CIrYc
fμI²eBalKWrukçCatibøg;tug køer:løaenH )anmkBIkardYsTwkBN’ébtgx©IcMnYn 75
lIRtBIRsHEdleTIbEt)anerobcMrYcry³eBl 2éf¶ edayeRbIR)as;brimaN
kMe)ar5KILÚRkamkñúg100Em:Rtkaer:RsHnigedGaeb:0/5KILÚRkamkñúg100
-2-

9. Em:Rtkaer:RsH enAmuneBlRbePTstVbøg;tugr:UTIhV½r (Rotifer) ekItmaneLIg.
bnÞab;mkykeTAdak;cUlkñúgFugcMNuH 1 Em:RtKubedayeRcaHnwgés,manTMhM
RkLa 5 mIRkUEm:Rt nigbEnßmTwkGNþÚg 75 lIRt edayeRcaHnwgés,Edlman
TMhMRkLa 5 mIRkUEm:RtdUcKña. bnÞab;mkeTot dak;CIeTAtamtaragTI 1 )an
bgðajCUnxageRkam. l,ayTwkkñúgFug)anerobrab;xagelIRtUvrkSaCeRmABI
15 eTA 20 sg;TIEm:Rt rYckUrva[)ancMnYnelIsBI 5 dg kñúgmYyéf¶kñúgry³
eBl 4 éf¶. enAéf¶TI 4 eyIgcab;epþImepÞrTwkébtg køer:løa (Chlorella sp.)
cMnYn 50 lIRt edayeRcaHés,TMhMRkLa 5 mIRkUEm:Rtdak;cUleTAkñúgGagmYy
epSgeTot edIm,IeFVIkarciBa©wmrukçCatibøg;tug køer:løary³eBl 4 éf¶eLIgvij.
2
2>>2
2 rreeb
boob
bc
ciiB
Baa©©wwm
mé
éc
cT
Twwk
k (Moina macrocopa)
RbPBemBUCécTwk eyIgGacrkTij)anBIraCFanIPñMeBj enAtamTIkEnøg
lk;RtIlMG. CaFmμta eyIgcMNayEt 2 duløar b:ueNÑaHGacTijécTwk)an
lμmRKb;RKan;sRmab;ykmkeRbIR)as;eFVICaemBUC)an. bnÞab;mkeyIgciBa©wm
rukçCatibøg;tug køer:løa ry³eBl 4 éf¶ sRmab;ykmkeRbIR)as;kñúgkarciBa©wm
begáInBUCécTwk. eyIgRtUvEfrkSaécTwkEdlciBa©wmenAkñúgGagenaHrhUtdl;
eBlNaTwkElgmanBN’ébtg eTIbGacRbmUl)an rYcepÞrvaeTAdak;ciBa©wm
bnþsarCafμIeLIgvijenAkñúgGagmYyepSgeTot. karciBa©wmrebobenHCaeRcIn
dg nigeRcInsar edIm,ITTYl)annUvBUCécTwkeRcInRKb;RKan;.
k
kMMN
Nt
t;;s
sMMK
Kaal
l;;³
³ edIm,IplitécTwk eyIgcaM)ac;dMbUgRtUveFVITwkEdldak;
ciBa©wmécTwkenaH[manBN’ébtgsin edayciBa©wmkøer:løa ¬Chlorella sp.¦ mun
nwgTij b¤ dak;ciBa©wmécTwk ¬Moina macrocopa¦enaH .
-3-

10. 3>kareRbIR)as;CIkñúgkarciBa©wmrukçCatibøg;tug nigécTwk
karciBa©wmrukçCatibøg;tug nigécTwkRtUv)andMeNIrkareFVIeLIgedayeRbI
R)as;nUvsmasFatupSM nigbrimaNCIdUcKña dUcbgðajCUnkñúgtaragTI 1 xag
eRkam³
t
taarraag
gT
TII 1
1 s
sm
maas
sF
Faat
tuup
pSSMMs
sRRm
maab
b;;c
ciiB
Baa©©wwm
mééc
cT
Twwk
k n
niig
grruuk
kççC
Caat
tiib
bøøg
g;;t
tuug
g
4> RbB½n§GagciBa©wmbøg;tug
enAkñúgmCÄmNÐlRsavRCav nigGPivDÆn_varIvb,kmμTwksab eyIg)an
eRbIR)as;GagcMNuHTwk 1 Em:RtKub cMnYn 5 GagsRmab;ciBa©wmrukçCatibøg;tug
køer:løa ¬rUbPaBTI 3¦ nigGagcMNuHTwk 13 Em:RtKub cMnYn 5 GagepSgeTot
sRmab;ciBa©wmécTwk ¬rUbPaBTI 4¦. tamry³Éksarbec©keTsenH eyIg´
sUmeFVIkarENnaMdl;ksikrplitkUnRtIBUCTaMgGs;enAtambNþaextþnana[eFVI
karsagsg;GagmanTMhMsmRsbsRmab;ciBa©wmBBYkbøg;tugenH edayeFVIy:ag
NaGacplitécTwk)ancMnYn 2 KILÚRkam y:agticbMputkñúgmYyéf¶.
-4-
smasFatupSM brimaN ¬kñúgGagcMNuHTwk 1 Em:RtKub¦
CIG‘uyer: 100 Rkam
CIedGaeb: 110 Rkam
knÞk; 100 Rkam
emSAsENþkesog 50 Rkam
emSARtI 50 Rkam

11. 4
4>>1
1 G
Gaag
gc
ciiB
Baa©©wwm
mrruuk
kççC
Caat
tiib
bøøg
g;;t
tuug
g k
køøeerr::l
løøaa
CadMbUgeyIgerobcMGagciBa©wmkøer:løacMnYn 5 EdlGagnImYy²manTMhM
1 Em:Rt x 2 Em:Rt x 0/7 Em:Rt bnÞab;mkeyIgbBa©ÚlTwkébtgcMNuH 0/5
Em:RtKub nigbEnßmTwkGNþÚgeTAkñúgGagciBa©wmkøer:løaxagelI[)anCeRmATwk
0/3 Em:Rt dUcbgðajkñúgKMnUstagTI 1 B. bnÞab;mkGagciBa©wmkøer:løa nig
GagrukçCatibøg;tug køer:løa TaMgenHKYrerobcM[mandMbUlsRmab;karBarTwk
ePøógEteFVIy:agNa[BnøWGaccUl)anRKb;RKan; edIm,ICaRbeyaCn_eFVI[rukçCati
bøg;tugmankarlUtlas; nigBN’ébtgl¥.
-5-
rrUUb
bP
PaaB
BT
TII3
3³³k
kaarrc
ciiB
Baa©©wwm
mk
køøeerr::l
løøaa
rry
y³³eeB
Bl
l 4
4 ééf
f¶¶
rrUUb
bP
PaaB
BT
TII4
4³³ k
kaarrc
ciiB
Baa©©wwm
mééc
cT
Twwk
k
rry
y³³eeB
Bl
l 4
4 ééf
f¶¶

12. -6-
K
KMMn
nUUs
st
taag
gT
TII 1
1³³ RRb
bB
B½½n
n§§G
Gaag
gc
ciiB
Baa©©wwm
mééc
cT
Twwk
k ¬A¦ ¬rrUUb
bP
PaaB
Bx
xaag
geeq
qVVg
g¦
¦n
niig
g
RRb
bB
B½½n
n§§G
Gaag
gc
ciiB
Baa©©wwm
mk
køøeerr::l
løøaa ¬B¦ ¬rrUUb
bP
PaaB
Bx
xaag
gs
sþþaaMM¦
¦

13. -7-
K
K
M
M
n
n
U
U
s
s
t
t
a
a
g
g
T
T
I
I
2
2
³
³
v
v
K
K
Á
Á
r
r
e
e
o
o
b
b
c
c
M
M
R
R
b
b
B
B
½
½
n
n
§
§
G
G
a
a
g
g
c
c
i
i
B
B
a
a
©
©
w
w
m
m
r
r
u
u
k
k
ç
ç
C
C
a
a
t
t
i
i
k
k
ø
ø
e
e
r
r
:
:
l
l
ø
ø
a
a
¬
¬
Chlorella
sp.
¦
¦
r
r
U
U
b
b
P
P
a
a
B
B
¬
A)
n
n
i
i
g
g
v
v
K
K
Á
Á
c
c
i
i
B
B
a
a
©
©
w
w
m
m
é
é
c
c
T
T
w
w
k
k
k
k
ñ
ñ
ú
ú
g
g
r
r
y
y
³
³
e
e
B
B
l
l
4
4
é
é
f
f
¶
¶
(Moina
macrocopa)
r
r
U
U
b
b
P
P
a
a
B
B
¬
B)

14. 4
4>>2
2 G
Gaag
gc
ciiB
Baa©©wwm
mé
éc
cT
Twwk
k
edIm,IplitécTwk[)an2KILÚRkamCaerogral;éf¶enaHeyIgRtUverobcM
RbB½n§GagciBa©wmcMnYn5EdlGagnImYy²manTMhM2/5Em:Rtx 5Em:Rtx0/7
Em:RtnigCeRmATwk0/3Em:RtdUcbgðajCUntamry³KMnUstagTI1A.cMeBaH
dMbUlGagciBa©wmécTwk KWeyIgeFVIdUcKñaeTAnwgdMbUlGagciBa©wmrukçCati bøg;tug
køer:løapgEdr.
5> karRKb;RKg
5
5>>1
1 k
kaarrc
ciiB
Baa©©wwm
mb
bøøg
g;;t
tuug
grruuk
kççC
Caat
tii ¬
¬Chlorella sp.¦
¦ rry
y³
³eeB
Bl
l4
4é
éf
f¶¶
enAéf¶dMbUg eyIgbBa©ÚlTwkGNþÚg
cMNuH 500 lIRt edayeRcaHnwgés,Edl
TMhMRkLa 5 mIRkUEm:Rt nigbEnßmTwk
køer:løacMnYn 50 lIRt cUleTAkñúgGag
ciBa©wmkøer:løa rYcbnÞab;mkeyIgdak;CI dUc
enAkñúgtaragTI 1 xagelI.
edIm,IeCosvagkMu[mansarFatupSM
kkenA)atGagenaH eyIgRtUveRbI]bkrN_
mü:ageFVIBIeQIragdUcrnas; ¬rUbPaBTI 6¦
sRmab;kUr[)aneRcInCag 5 dg kñúgmYy
éf¶ cab;BIéf¶dMbUgrhUtdl;éf¶TI 4.
eRkayry³eBl 3 éf¶ b¤ elIsBIenH enAkñúgkarciBa©wmrukçCatibøg;tug
køer:løa TwknwgERbeTACaBN’ébtgcas;. enAéf¶TI 4 eyIgGacRbmUlpl)an
edayCadMbUg RtUvbUmTwkébtgecjBIGagcMnYn 50 lIRt dak;cUleTAkñúgGag
-8-
rrUUb
bP
PaaB
BT
TII6
6³³ ]
]b
bk
krrN
N__rrn
naas
s;;k
kUUrrT
Twwk
k

15. mYyepSgeTotsRmab;eFVICaemBUCciBa©wmbnþeTot. rIÉTwkébtgenAsl;kñúg
GagciBa©wmcMnYn 500 lIRt enaHeyIgRtUvepÞrvadak;cUleTAkñúgGagciBa©wmécTwk
navKÁfμIbnþeTot. mü:ageTot eyIgRtUvrkSakRmitsItuNðPaBTwk[sßitenA
cenøaHBI 27 eTA 31 GgSaes kñúglkçxNÐRbB½n§GagciBa©wmkøer:løamanRbk;
dMbUlCakarRbesIr.
5
5>>2
2 k
kaarrc
ciiB
Baa©©wwm
mé
éc
cT
Twwk
k (Moina macrocopa) rry
y³
³eeB
Bl
l 4
4 é
éf
f¶¶
enAéf¶dMbUg eyIgbBa©ÚlTwkGNþÚgcMNuH 3>000 lIRt edayeRcaHnwg
és,ceRmaH nigTwkrukçCatibøg;tug køer:løa cMnYn 500 lIRt cUleTAkñúgGag
ciBa©wmécTwk bnÞab;mkeyIgdak;CIkñúgbrimaNdUcenAkñúgtaragTI1.enAéf¶TI2
eyIgdak;emBUCécTwkcMnYn 500 Rkam cUleTAkñúgGagciBa©wm ehIyrhUtdl;éf¶
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