2. ENERGY VALUE OF FOOD
• A calorie or kilocalorie is a measure of heat that express a
foods energy value.
• One calorie expresses the quantity of heat necessary to raise
the temperature of l kg of water by 1degree celsius
• An instrument for measuring heat output of the body or the
energy value of foods is called a Calorimeter.
Bomb calorimeter
• Measures total energy value of food.
• It is a type of direct calorimetry.
• Sealed chamber charged with oxygen. Increase in water
temperature directly reflects the heat released during a food’s
oxidation
• Heat of combustion.
3. Heat of combustion of a substance is defined as “the change in
enthalpy of a system when one mole of the substance is
completely burnt in excess of air or oxygen”.
• It is denoted by ( ΔH ∘ C)
• Heat of combustion for :
1g of Carbohydrate - 4.2 kcal
1g of fat - 9.45 kcal
1g of protein - 5.65 kcal.
NET ENERGY VALUE :
Actual energy available to the body
Coefficient of digestibility is affected by dietary fiber
Atwater general factors
4. • The Atwater system is used in the food industry to determine
the total calorific value of food.
• Atwater 4-9-4 kcal rule generally proves useful to estimate
the intake of food energy and content of the daily diet.
4kcal per gram for dietary carbohydrate
9kcal per gram for dietary lipid
4 kcal per gram for dietary protein
6. • Bio energetics refers to the flow and exchange of energy
within a living system
• FIRST LAW OF THERMODYNAMICS :
It is also known as law of
conservation of energy . Energy can not be created or
destroyed, but is transformed from one form to another
without being depleted.
• SECOND LAW OF THERMODYNAMICS :
It is also known as law of
degradation of energy. The transfer of potential energy to
kinetic energy in any spontaneous process always proceeds in
a direction that decreases the capacity to do work
7.
8. INTERCONVERSIONS OF ENERGY:
EXAMPLES OF ENERGY CONVERSIONS
photosynthesis
captures energy to utilize as food and oxygen.
Cellular respiration
part of the energy released becomes conserved
in compounds used for biologic process.
9.
10. ENERGY TRANSFER IN THE BODY
• Energy transfer in human supports three forms of
biologic work :
• Mechanical work of muscle contraction
chemical energy → mechanical energy
• chemical work : neurotransmission and synthesize
cellular molecule
chemical energy → electrical energy
• Transport work:
diffusion
Active transport
11. THE RATE OF BIOENERGETICS :
• Enzymes act as biological catalysts
Reduce required activation energy
Accelerate the rate of chemical reactions
Reaction rates depend on PH, temperature , availability of
substances
ENZYMES
mode of action:
enzyme -substrate complex
COENZYMES:
contains non protein, organic substances
assist enzyme action by binding the substrate to the enzyme.
ENZYME INHIBITION:
competitive inhibitors – bind to active site
non competitive inhibitors- bind to a nonactive site
12. HYDROLYSIS AND CONDENSATION:
Hydrolysis reactions
catabolism of complex organic molecules
split chemical bonds by adding H+ and OH-
Condensation reactions
anabolism of complex biomolecules.
reverse of hydrolysis
OXIDATION AND REDOX REACTIONS:
OXIDATION – loss of electrons
transfer of oxygen, hydrogen,electrons
REDUCTION- gain of electrons
REDOX REACTIONS:
oxidation and reduction are coupled.
14. Carbohydrates primary function -supplies energy for cellular
work.
Maximal exercise - rapid energy release supplied by aerobic
metabolism.
During light and moderate aerobic exercise, supplies
about one third of the body’s energy requirements.
In prolonged aerobic exercise such as marathon running,
athletes often experience nutrient-related fatigue— muscle and
liver glycogen depletion.
depleting glycogen reduces exercise power output.
ENERGY RELEASE FROM CARBOHYDRATES
15. ENERGY RELEASE FROM FAT
• Stored fat represents the source of potential energy. Adipocyte
is the Site of Fat Storage and Mobilization
• two sources:
triacylglycerol in fat cells (adipocytes)
intramuscular triacylglycerol
Three specific energy sources for fat catabolism include:
1. Triacylglycerols stored directly within the muscle fiber
2. Circulating in lipoprotein complexes become hydrolyzed
3. Mobilization
utilizing fatty acids is lipolysis
triacylglycerol splits into fatty acids and glycerol
hormone sensitive lipase drives lipolysis.
16. ENERGY RELEASE FROM PROTEIN
DEAMINATION:
• nitrogen removal from amino acid
• occurs in liver and muscles
• enter citric acid cycle for oxidation.
TRANSAMINATION
• amine group transferred
Proteins as a fuel source :
glucogenic may be used to form( pyruvate,oxaloacetate,
malate )
ketogenic may be used to form (acetyl-CoA , Acetoacetate)
18. Immediate Energy
• The ATP-PCr System
• 5-8 seconds of maximum intensity exercise.
• Sprinting, football, weight lifting, baseball, volleyball,etc.
Short-Term Energy
• Lactic Acid System
• Maximal exercise between 60-180 seconds.
• high lactate concentration in maximal exercise
increases with specific sprint and power training.
• 400 m run,100 m swim, Multiple sprint sports ice
hockey, field hockey and soccer.
19. Lactate Accumulation
• Onset of blood lactate accumulation (OBLA)
• Blood lactate threshold (blood lactate is an Energy
Substrate for Gluconeogenesis in liver)
• Anaerobic threshold
Long Term Energy
• the Aerobic System
• Oxygen uptake (VO2).
• during the first minute of exercise.- Oxygen uptake
rises rapidly
• Between 3rd and 4th minute - a plateau is reached
and oxygen uptake remains stable.
• Functional capacities - to deliver adequate oxygen to
muscles
20. OXYGEN DEFICIT:
• The difference between oxygen uptake of the body during
early stages of exercise and during a similar duration in a
steady state of exercise aerobic metabolism occurs at onset of
exercise.
OXYGEN DEFICIT IN TRAINED AND UNTRAINED
• oxygen uptake during light and moderate intensity exercise
• trained reaches steady rate quicker
• Higher total energy consumption
• Less reliance on anaerobic gylcolysis
• Lower deficit in trained individual due to :
– Earlier aerobic ATP production
– Less lactate formation
21. Oxygen Debt
• The amount of extra oxygen the body needs
after exercise to react with the build up of
lactic acid and remove it from the cells.
Lactic acid + oxygen→ carbon dioxide +water
Lactic acid is poisonous (it also causes
muscle soreness) and needs to be removed.
• Breathing rate and heart rate remain high
after stopping exercise to “pay back” the
oxygen debt.
22. Maximum Oxygen Uptake
• The point when VO2 plateaus with additional workloads.
• Maximum VO2 indicates -aerobic resynthesis of ATP.
• above the max VO2 - accomplished by anaerobic
glycolysis.
Fast- and Slow-Twitch Fibers
Fast twitch fibers (ii) Slow twitch fibers (i)
Fast contraction speed Half as fast as fast twitch
High anaerobic capacity High aerobic capacity
23. Energy Spectrum
• Relative contribution of aerobic and anaerobic
energy during maximal physical effort.
• Intensity and duration determine the blend.
• Nutrient-related Fatigue : severe depletion
glycogen.
Oxygen consumption during Recovery
• Light aerobic exercise: steady-rate and small oxygen
deficit.
• Moderate to heavy: steady-rate and large oxygen deficit.
24. EXCESS POST- EXERCISE OXYGEN CONSUMPTION (EPOC)
• excess oxygen above the resting level is recovery.
• Oxygen deficit is smaller in moderate exercise
• Lactic acid accumulates in strenuous exercise
• Body temperature increased
Traditional Oxygen Debt Theory
• Alactacid oxygen debt: restoration of ATP& PCr
depleted during exercise, small portion to reload
muscle myoglobin and hemoglobin (fast).
• Lactacid oxygen debt: restoration of glycogen by
resynthesizing 80% HLa through gluconeogenesis (Cori
cycle) and to catabolize through pyruvic acid (Krebs cycle)
slower phase.
25. Lactate removal post – exercise
• mass action effect
• passive recovery (steady rate)
• active recovery( non steady rate)
Exercise Recovery Ratio
• 1:3 ratio overloads immediate energy system
• 1:2 ratio to train short-term glycolytic system
• 11 ratio to train long-term aerobic system
27. Direct calorimetry
Airflow calorimeter
Water flow calorimeter
Gradient layer calorimeter
Storage calorimetry
Indirect calorimetry
closed circuit spirometry
Open circuit spirometry
Bag technique
Computerized instrumentation
Doubly layered water technique
Respiratory exchange ratio
Respiratory quotient
28. DIRECT CALORIMETRY :
• When body used energy to do work, heat is liberated
• Foodstuffs +O2 →ATP + Heat
↓cell work
Heat
• commonly heat production is measured in calories
1kcal=1000 calories
• This heat production can be measured directly in the Bomb
calorimeter.
• In this technique place human in air tight insulated chamber
with cold water flowing at constant rate , difference water
temperature in & out of chamber equals heat production.
•40% of the energy liberated from CHO/fat metabolism is used to
produce ATP & 60% is dissipated as heat
29. Changes in water temperature relate directly to an individual’s
energy
An increase of one degree Celsius by each kg (liter) of water
flowing equals one Kcal.
CO2 is removed by chemical absorbents.
30.
31.
32. INDIRECT CALORIMETRY
It is a technique used to measure the metabolic rate of
an individual by indirectly measuring their oxygen
consumption (vo2) and carbon dioxide production
(vco2).
Foodstuffs + O2 & Heat + CO2 + H₂O
(indirect) (direct)
body consumes oxygen to produce energy in the form
of ATP and this process results in the production of
carbon dioxide as a byproduct.
Measurement of oxygen consumption is indirect, heat
not measured directly.
33. Closed circuit spirometry :
• spirometer filled with oxygen is used, Patient
breathes in and out
• Soda lime is used to absorb carbon dioxide.
Open circuit spirometry
• portable spirometry
• Spirometer is small, air volume is metered.
• It is used to measure concentrations of gases.
• Volume of carbon dioxide consumed per minute is
calculated as volume CO2 expired – volume CO2
inspired.
34. Doubly Labeled Water Technique
Isotope-based method
Doubly labeled water contains
Oxygen-18
Deuterium
Isotopes consumed in a known volume of water
Isotopes distribute throughout body fluids
Hydrogen leaves body as 2H2O in sweat and urine .
Oxygen leaves body as C18O2 orH2
18O
Estimates total daily energy expenditure
35. Respiratory Exchange Ratio (RER)
• Ratio of CO2 produced to O2 consumed
• Calculation of RER is the same as RQ
Metabolic calculations
• Calculating energy expenditure during exercise
• Volume of air
• Concentration of O2 and CO2
Respiratory quotient
(RQ) is ratio of volume of carbon dioxide produced to volume
of oxygen consumed.
RQ = CO2 produced / O2 consumed
37. BASAL METABOLIC RATE:
BMR is the minimum amount of energy needed by the
body at rest in the fasting state
measurements are taken by waking after 8 hours of sleep
and 12 hours of fasting
Basal energy expenditure BEE or BMR is determined
largely by body size, body composition, Gender and age.
Lower in females compared to males
BMR is typically measured by indirect calorimetry
Reflected in heat production
Determined by oxygen consumption
Normalcy of BMR Value
Δ BMR = (measured BMR - standard BMR) x100 ÷
standard BMR
38. RESTING METABOLIC RATE:
• The amount of energy used by a person in 24 hours when at
rest 3_4 hrs after a meal
• active metabolic processes & regulatory balance at rest
• accounts for 60-75% of TDEE
• physical activity 15-30%
• percent of the total daily calorie expenditure
Harris-Benedict equation : formula used to calculate BEE:
• Adult males: • BEE (kcal/day) = 66 + (13.7 x wt in kg) + (5 x ht in
cm) - (6.8 x age).
• Adult females: • BEE (kcal/kcal) = 655 + (9.6 x wt in kg) + (1.7 x
ht in cm) - (4.7 x age).
39. The thermic effect of food (TEF) :
• estimation from eating food, increase in energy expended
above RMR or BMR that results from digestion, absorption,
and storage of the food.
• It is also called the specific dynamic effect (SDE) of food
or the specific dynamic activity (SDA) of food
• The sum of the TEF and any increase in the metabolic rate due
to overeating is known as diet-induced thermogenesis
• 5-10 percent of the total calories burn in a day
effect of foods:
• Carbohydrate: 5–10%
• Fat: 0–5% is very easy to process &very little thermic effect
• Protein: 20–30% is hard to process & much larger thermic
effect
• Alcohol: 15–20%
40. FACTORS THAT AFFECT ENERGY
EXPENDITURE:
• physical activity
• dietary- induced thermogenesis
obligatory- digestive process
facultative- sympathetic process
• climate
hot or cold climate increase energy
expenditure
• pregnancy
increase BMR due to weight gain
41. ENERGY EXPENDITURE DURING
PHYSICALACTIVITY
CLASSIFICATION OF PHYSICAL ACTIVITIES BY ENERGY
EXPENDITURE
intensity
duration
physical activity ratio (PAR)
light work – 1-3xBMR
heavy work – 6-8 xBMR
maximal work – > 9 timesXBMR
THE MET:
MET = metabolic equivalent
• the amount of energy expended during exercise relative to
theenergy expenditure during rest
1 MET= 3.5 mL x Kg-1 x min -1
42.
43.
44.
45.
46. References
• McArdle, William D., Frank I. Katch, and Victor
L. Katch. 2000. Essentials of Exercise
Physiology 2nd ed. Image Collection. Lippincott
Williams Wilkins.
• Plowman, Sharon A. and Denise L. Smith. 1998.
Digital Image Archive for Exercise Physiology.
Allyn Bacon.