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Acta Hortic. 1194. ISHS 2018. DOI 10.17660/ActaHortic.2018.1194.95
Proc. VIII International Postharvest Symposium:
Enhancing Supply Chain and Consumer Benefits – Ethical and Technological Issues
Eds.: F. Artés-Hernández et al.
665
Quality and shelf-life improvement of fresh strawberry
(Fragaria sp. ‘Holibrite’) using Aloe vera coating during
storage in a tropical environment
M.A.F.	Falaha,	P.	Yuliastuti,	Jumeri	and	N.	Khuriyati	
Faculty	of	Agricultural	Technology,	GadjahMada	University,	Yogyakarta,	Indonesia.	
Abstract
Fresh	 strawberry	 (Fragaria	 sp.	 ‘Holibrite’)	 sharply	 decrease	 their	 quality	 and	
also	have	a	short	shelf-life	in	a	tropical	environment	conditions.	The	coating	method	
using	Aloe vera	was	applied	to	strawberry	fruit	to	preserve	their	quality	and	increase	
their	shelf	life.	Aloe vera	is	used	as	a	biopolymer	coating	to	cover	strawberry	fruit	skin	
with	 standard	 formulation	 as	 gel	 coating.	 Different	 maturity	 levels	 of	 strawberry	
fruits	were	used	and	the	quality	parameters	of	fresh	strawberry	assayed	were	weight	
loss	 percentage,	 skin	 texture,	 water	 content,	 titratable	 acidity,	 vitamin	 C	 and	 total	
soluble	solid	content.	Storage	 temperatures	 were	set	at	4°C	with	 RH	62%	and	10°C	
with	RH	68%	and	then	compared	with	the	sample	at	room	temperature	as	control	for	
tropical	 environment.	 All	 quality	 parameters	 of	 strawberry	 fruit	 coated	 using	 Aloe
vera	could	be	kept	stable	at	controlled	temperature	storage.	Moreover,	the	shelf	life	of	
strawberry	 fruit	 under	 controlled	 storage	 temperatures	 was	 longer	 than	 those	 at	
tropical	 environment	 conditions.	 Aloe vera	 coating	 can	 preserve	 quality	 conditions	
and	increase	shelf	life	of	strawberry	fruit	in	a	tropical	controlled	storage	temperature	
and	at	room	temperature.	
Keywords:	Aloe	vera	coating,	quality,	strawberry,	shelf-life,	tropical	environment	
INTRODUCTION	
Strawberry	(Fragaria	sp.)	are	a	unique	and	popular	fruit	with	highly	desirable	taste	
and	flavour,	visual	appeal,	excellent	dietary	sources	of	ascorbic	acid,	potassium,	fibre,	simple	
sugar	as	sources	of	energy	and	highly	perishable	products	that	lose	sensory	quality	shortly	
after	 harvest,	 being	 susceptible	 to	 mechanical	 injury,water	 loss,	 decay	 and	 physiological	
deterioration	which	limits	their	shelf	life	to	a	few	days	at	ambient	temperature	(Azodanlou,	
et	 al.,	 2003;	 Giampieri	 et	 al.,	 2012).	 Production	 of	 strawberry	 in	 Indonesia	 as	 a	 tropical	
country	was	increased	24%	in	2011-2013	and	they	were	cultivated	in	valley	and	mountain	
areas	such	as	in	Central	Java	(Purbalingga,	Magelang	and	Semarang),	in	East	Java	(Malang,	
Bondowoso	and	Batu),	in	West	Java	(Ciwidey	and	Garut)	and	also	Brastagi,	North	Sumatera	
(Anon.,	2012;	Hanif	et	al.,	2011).	Local	strawberry	in	Indonesia	from	Ciwidey,	Garut,	West	
Java,	were	strongly	recommended	to	store	in	lower	temperature	conditions,	around	10°C,	to	
maintain	their	quality	(Falah	et	al.,	2016).	However,	in	a	tropical	environment,	postharvest	
losses	of	horticultural	products	such	as	strawberry	fruit	is	one	of	the	main	problems.	This	
loss	of	quality	and	quantity	of	the	horticultural	product	through	their	rapid	deterioration	
occurs	 during	 handling,	 transportation	 and	 also	 storage.	 To	 overcome	 these	 problems	
several	 treatments	 were	 applied	 by	 the	 producer	 and	 fresh	 industrial	 user	 during	 their	
chain.	Cooling	treatment	is	one	of	the	main	treatments	to	preserve	and	maintain	the	quality	
and	quality	of	the	fruit,	furthermore,	edible	coating	is	one	of	the	alternative	treatments	that	
is	applied	nowadays.	
Application	of	edible	coatings	has	been	shown	to	be	promising	as	a	tool	to	improve	the	
quality	and	extend	storage	as	well	as	shelf	life	of	various	fruit	such	as	papaya	(Marpudi	et	al.,	
2011),	table	grapes	(Valverde	et	al.,	2005),	tomato	(Athmaselvi	et	al.,	2013),	sweet	cherry	
(Martı́nez-Romero	 et	 al.,	 2006)	 and	 strawberries	 (Vahdat	 et	 al.,	 2010;	 Zafari	 et	 al.,	 2015;	
a
E-mail: affan_tip@ugm.ac.id
666
Sogvar	 et	 al.,	 2016).	 Edible	 coatings	 have	 demonstrated	 the	 capability	 of	 improving	 food	
quality	 and	 prolonging	 shelf	 life	 of	 fresh	 produce	 through	 regulating	 the	 transfer	 of	
moisture,	oxygen,	carbon	dioxide,	aroma,	and	taste	compounds	in	a	food	system	(Castillo	et	
al.,	2010),	and	they	can	act	as	moisture	and	gas	semi-permeable	barriers,	resulting	in	control	
of	microbial	growth,	preservation	of	color	and	texture	(Bourtoom,	2008).	One	of	the	novel	
edible	coatings	to	maintain	the	quantity	and	quality	of	horticultural	products	is	Aloe	vera	
(Serrano	et	al.,	2006;	Valverde	et	al.,	2005).	Aloe	vera	has	antifungal	activity	against	several	
pathogenic	 fungi	including	Botrytis	cinerea	(De	Rodrıguez	 et	 al.,	2005).	Aloe	vera	coatings	
modify	the	internal	gas	atmosphere,	reduce	moisture	loss,	softening,	respiration	rates,	delay	
oxidative	browning	and	reduce	microorganism	proliferation	in	fruit	such	as	papaya,	sweet	
cherries,	table	grapes,	and	also	strawberries	(Marpudi	et	al.,	2011;	Martı́nez-Romero	et	al.,	
2006;	Valverde	et	al.,	2005;	Vahdat	et	al.,	2010;	Zafari	et	al.,	2015;	Sogvar	et	al.,	2016).	
In	this	study,	we	investigated	the	effects	of	edible	coating	using	Aloe	vera	treated	in	
various	temperature	conditions	to	several	quality	parameters	of	fresh	strawberry	(weight	
loss	percentage,	skin	texture,	water	content,	titratable	acidity,	vitamin	C	and	total	soluble	
solid	content)	that	were	produced	using	soil	cultivation	in	a	tropical	environment.	
MATERIALS	AND	METHODS	
Plant	materials	
Strawberry	 (Fragaria	 sp.	 ‘Holibrite’)	 fruit	 were	 produced	 by	 conventional	 method	
using	soil	cultivation,	located	in	Ketep	Pass	Banyuroto	Village,	Sawangan	District,	Magelang,	
Central	Java,	Indonesia.	This	farm	is	located	between	longitude	110°01’51”	and	110°26’58”E	
and	 latitude	 between	 7°19’13”	 and	 7°42’16”S	 in	 the	 highland	 with	 1200	 m	 a.s.l.,	 with	
climatic	conditions	in	average	air	temperature	18-25°C	and	average	relative	humidity	of	80-
90%.	Strawberry	fruit	maturation	had	three	different	types,	first	type	was	full	maturation	
with	more	than	80%	red	color	of	the	skin	strawberry	with	age	of	the	fruit	around	30	days	
after	flowering	(F),	second	type	was	66%	red	color	of	the	skin	strawberry	with	age	of	the	
fruit	around	25	days	after	flowering	(F1)	and	third	type	was	33%	red	color	of	the	skin	of	
strawberry	with	age	of	the	fruit	around	20	days	after	flowering	(F2).	
Edible	coating	formulation	and	environmental	conditions	
Edible	 coating	 of	 Aloe	vera	 had	 three	 different	 formulations,	 all	 formula	 used	 0.5%	
carboxymethyl	cellulose	(CMC)	and	2.5%	of	corn	syrup	that	was	added	to	each	formula.	The	
first	formula	had	a	composition	of	25%	Aloe	vera,	25%	ethanol,	and	47%	water	(AV1),	the	
second	formula	had	a	composition	of	32.5%	Aloe	vera,	32.5%	ethanol,	and	32%	water	(AV2)	
and	the	third	formula	had	a	composition	of	40%	Aloe	vera,	40%	ethanol,	and	17%	water	
(AV3).	During	formulation	of	the	edible	coating	solution,	the	solution	was	mixed	and	stirred	
with	hot	water	at	80±2°C	for	30	min,	then	fruit	were	dipped	in	solution	of	edible	coating	
formula	with	immersion	time	of	3	min,	drained	by	using	perforated	plastic	containers,	and	
air-dried	over	nylon	screen	for	90	min.	Edible	coating	of	strawberry	fruit	was	then	wrapped	
in	three	types	of	packaging,	first	unperforated	packaging	(UP),	perforated	packaging	(P)	and	
unperforated	packaging	with	cardboard	mat	(UPC),	same	as	the	condition	of	strawberry	in	
the	market.	Furthermore,	these	strawberry	fruit	with	Aloe	vera	edible	coating	were	stored	in	
different	 conditions	 of	 temperature,	 ambient	 temperature	 (27°C),	 distribution	 centre	
temperature	 (10°C)	 and	 supermarket	 temperature	 (4°C).	 Strawberry	 fruits	 with	 edible	
coating	were	compared	with	those	without	coating	at	the	same	temperature	conditions	that	
were	 mentioned	 before.	 We	 measured	 physical	 and	 nutritional	 characteristics	 of	 these	
strawberries	for	several	days	until	conditions	of	the	strawberry	fruit	were	not	acceptable	
anymore	to	be	consumed.	
Physical	and	nutritional	quality	evaluation	of	strawberry	
Quality	 evaluation	 of	 fresh	 strawberry	 fruit	 was	 done	 based	 on	 their	 physical	 and	
nutritional	 characteristics.	 For	 physical	 evaluation,	 weight	 loss	 percentage	 was	 measured	
using	a	balance	(T	and	D,	Co.	Ltd.,	Osaka,	Japan)	where	the	formula	is	[(FW1–FW2)/FW2)
  667
×100%],	FW1	is	fresh	weight	at	the	first	day	measurement	and	FW2	is	fresh	weight	at	the	
second	 day	 of	 measurement.	 Texture	 of	 strawberry	 fruit	 skin	 as	 fruit	 firmness	 was	
determined	using	a	texture	analyzer	(UTM,	Model	ZwickTipe	DO	FB0	5TS,	Germany).	Fresh	
weight	 measurements	 showed	 the	 average	 data	 from	 1	 packaged	 sample	 containing	 18	
strawberry	fruit	for	samples	and	5	samples	of	strawberry	fruit	with	triplicates	were	used	for	
texture	measurement.	The	strawberry	fruit	were	then	evaluated	until	the	strawberry	fruits	
were	not	acceptable	anymore	to	be	eaten.	
For	 nutritional	 evaluation,	 water	 content	 was	 evaluated	 using	 a	 thermogravimetri	
method	 (Sato	 et	 al.,	 2006),	 soluble	 solid	 content	 (%	 Brix)	 was	 measured	 using	 a	
refractometer	(PAL-1,	Atago	Co.	Ltd.,	Japan),	acidity	of	fruits	was	measured	using	the	value	
of	 titratable	 acidity	 method	 and	 ascorbic	 acid	 or	 vitamin	 C	 was	 determined	 using	 the	
indophenols	titration	method.	All	nutritional	measurements	used	a	standard	method	(AOAC,	
1990),	and	they	were	evaluated	for	5	days	and	the	average	data	from	5	samples	for	each	
measurement	were	used	with	triplicates.	Data	obtained	from	the	experiment	results	were	
tabulated	 and	 calculated	 using	 Microsoft	 Excel	 2007	 (Microsoft	 Corporation).	 Further	
statistical	 analysis	 utilized	 SPSS	 version	 13.0	 (SPSS	 Incorporation)	 to	 obtain	 optimum	
treatments	and	significance	of	the	obtained	results	through	 analysis	of	 variance	(ANOVA)	
and	LSD	interpretations.	
RESULTS	AND	DISCUSSION	
Harvested	 fresh	 strawberry	 fruit	 as	 a	 sample	 were	 transported	 around	 1	 h	 to	 the	
Faculty	 of	 Agricultural	 Technology	 Gadjahmada	 University	 Yogyakarta,	 Indonesia.	 The	
samples	of	strawberry	fruit	were	then	coated	using	Aloe	vera	with	several	treatments	and	
different	 conditions	 of	 temperature	 during	 their	 storage.	 Physical	 and	 nutritional	 quality	
parameters	 of	 strawberry	 fruit	 coated	 with	 Aloe	 vera	 were	 evaluated	 based	 on	 the	
treatments	 and	 conditions	 mentioned	 above,	 and	 they	 were	 compared	 with	 control	
strawberry	without	coating.	
Physical	characteristics	of	strawberry	fruit	quality	
Figure	 1	 shows	 physical	 characteristics	 of	 weight	 loss	 percentage	 from	 strawberry	
fruit	during	storage	under	different	temperature	conditions	and	different	Aloe	vera	coating	
treatments	and	compared	with	control	strawberry	fruit	at	specific	temperature	conditions	
without	Aloe	vera	coating	treatments.	At	lowest	temperature	conditions	(4°C),	coating	with	
Aloe	vera	had	different	patterns,	where	weight	loss	percentage	of	AV3	was	higher	than	AVI,	
however,	both	of	them	showed	losses	in	fresh	weight	faster	than	the	control	temperature.	
However,	control	without	Aloe	vera	coating	at	the	lowest	temperature	had	the	shortest	shelf	
life.	We	supposed	that	the	lowest	temperature	at	4°C	can	affect	physiology	and	respiration	of	
the	 strawberry	 fruit	 and	 coating	 with	 Aloe	 vera	 can	 maintain	 their	 quality	 from	 the	
unfavorable	effect	of	the	surroundings’	temperature,	although	chilling	injury	did	occur.	At	
the	lowest	temperature	 of	the	coating	with	Aloe	vera,	strawberry	 fruit	can	 maintain	their	
shelf	life	and	are	still	acceptable	to	be	eaten	10	days	after	storage.	At	the	middle	temperature	
conditions	(10°C),	fresh	strawberry	coated	with	Aloe	vera	(AV3)	had	the	longest	shelf	life	
conditions	 (14	 days).	 We	 suggest	 that	 at	 the	 middle	 temperature	 conditions	 Aloe	 vera	
coating	 can	 maintain	 their	 weight	 loss	 better	 without	 chilling	 injury.	 At	 this	 temperature,	
control	of	fresh	strawberry	without	coating	had	a	shorter	shelf	life	compared	to	those	with	
coating.	Furthermore,	Figure	1	also	shows	coating	with	Aloe	vera	in	the	tropical	temperature	
conditions	can	reduce	weight	loss	percentage	and	extend	shelf	life	conditions.	
Figure	1	shows	physical	characteristics	on	change	of	texture	of	strawberry	fruit	skin	
during	 storage	 under	 different	 temperature	 conditions	 and	 different	 Aloe	 vera	 coating	
treatments	and	compared	with	control	strawberry	fruit	at	specific	temperature	conditions	
without	 Aloe	 vera	 coating	 treatments.	 The	 texture	 of	 the	 skin	 of	 fresh	 strawberry	 was	
relatively	more	firm	in	the	lowest	temperature	compared	with	the	middle	temperature	or	
tropical	 temperature.	 Coating	 with	 Aloe	 vera	 also	 slowly	 softened	 of	 the	 skin	 of	 fresh	
strawberry	 and	 increased	 shelf	 life	 of	 strawberry	 fruit	 compared	 to	 without	 coating	 at	
tropical	 room	 temperature.	 Firmness	 decreased	 relatively	 little	 at	 middle	 and	 lowest
668
temperature	in	strawberries	uncoated	and	coated	with	Aloe	vera.	These	conditions	were	also	
similar	to	those	reported	by	Sogvar	et	al.	(2016).	Longest	shelf	life	and	stable	texture	of	the	
skin	of	the	strawberry	occurred	in	the	 Aloe	vera	coating	of	 AV3	treatments	at	the	middle	
temperature	(10°C).	Decreasing	skin	texture	or	firmness	and	Aloe	vera	coating	making	the	
change	of	texture	of	the	skin	slower	can	be	associated	with	the	barrier	of	Aloe	vera	coating	
and	 the	 O2	 uptake	 mechanism	 (Bhaskara	 Reddy	 et	 al.,	 2000).	 Aloe	vera	 coating	 of	 fresh	
strawberry	can	maintain	the	physical	quality	of	fresh	strawberry	at	different	conditions	of	
temperature,	 specifically	 in	 tropical	 environment	 conditions	 without	 control,	 they	 can	
protect	 weight	 loss	 percentage,	 texture	 of	 the	 skin	 and	 then	 can	 maintain	 quality	 and	
increase	shelf	life.	
0%
20%
40%
60%
80%
100%
1 2 3 4 5 6 7 8 9 10
Weight	loss	
percentage(%)
AV1
AV2
AV3
K4
0
2
4
6
8
1 2 3 4 5 6 7 8 9 1011121314Textureofskin(N)
AV1
AV2
AV3
K4
0%
20%
40%
60%
80%
100%
1 2 3 4 5 6 7 8 9 10
Weight	loss	
percentage(%)
AV1
AV2
AV3
K10
0
2
4
6
8
1 2 3 4 5 6 7 8 9 1011121314
textureofskin(N)
AV1
AV2
AV3
K10
0%
20%
40%
60%
80%
100%
1 2 3 4 5
Weightloss
percentage(%)
Days	of	storage	
AV1
AV2
AV3
K27
0
2
4
6
8
1 2 3 4 5 6 7
Textureoftheskin(N)
Days	of	storage
AV1
AV2
AV3
K27
	
Figure	1.	 Physical	 characteristics	 (weight	 loss	 percentage	 and	 texture	 of	 the	 skin)	 of	
strawberry	 fruit	 during	 storage	 under	 different	 storage	 temperature	 conditions	
(4,	10	and	27°C)	and	different	Aloe	vera	coating	treatments	(AV1,	AV2	and	AV3).	K	
indicates	 control	 at	 specific	 temperature	 conditions	 without	 Aloe	 vera	 coating	
treatments.	The	data	are	an	average	from	18	sample	measuements	of	weight	loss	
and	5	sample	measurements	of	skin	texture.	
Nutritional	characteristics	of	strawberry	fruit	quality	
Figure	 2	 shows	 nutritional	 characteristics	 of	 water	 content	 from	 strawberry	 fruit	
during	 storage	 under	 different	 temperature	 conditions	 and	 different	 Aloe	 vera	 coating	
treatments	then	compared	with	control	strawberry	fruit	at	specific	temperature	conditions	
without	 Aloe	vera	 coating	 treatments.	 Water	 content	 of	 strawberry	 fruit	 coated	 with	 Aloe	
vera	was	not	significantly	different	with	strawberry	fruit	without	coating	at	the	lowest	and	
middle	 temperature	 conditions.	 However,	 water	 content	 of	 strawberry	 fruit	 in	 tropical	
conditions	 without	 Aloe	vera	 coating	 showed	 a	 small	 decrease	 although	 not	 significantly
  669
different.	
Figure	2	also	shows	nutritional	characteristics	of	acidity	from	strawberry	fruit	during	
storage	under	different	temperature	conditions	and	different	Aloe	vera	coating	treatments.	
The	 acidity	 content	 of	 strawberry	 fruit	 was	 significantly	 different	 under	 different	
temperature	 conditions,	 where	 at	 higher	 temperature,	 their	 acidity	 content	 was	 lower	
compared	with	strawberries	coated	with	Aloe	vera.	At	middle	and	lowest	temperature	(10	
and	 4°C),	 acidity	 content	 of	 strawberry	 fruit	 without	 coating	 was	 relatively	 similar	 with	
those	coated	with	Aloe	vera.	Coating	 with	Aloe	vera	in	combination	with	 lower	controlled	
temperature	 (10	 and	 4°C)	 can	 maintain	 strawberries’	 acidity,	 and	 will	 extend	 shelf	 life	 of	
fresh	strwberry	fruit.	The	slow	decline	of	acidity	content	with	Aloe	vera	coating	under	low	
temperature	might	be	supposed	through	metabolic	changes	in	fruit	resulting	from	organic	
acids	used	in	the	respiratory	process	(Echeverria	and	Valich,	1989)	and	the	Aloe	vera	coating	
effect	 on	 acidity	 content	 of	 strawberry	 fruit	 could	 produce	 modification	 of	 internal	
atmosphere	(Martı́nez-Romero	et	al.,	2006)	during	their	storage	in	combination	with	lower	
temperature	storage.	
20%
40%
60%
80%
100%
1 3 5 7 9 11 13
Watercontent(%)
AV1
AV2
AV3
K4
0
2
4
6
8
10
12
1 3 5 7 9 11 13
Titratableacidity
(mg/100g)
AV1
AV2
AV3
K4
20%
40%
60%
80%
100%
1 3 5 7 9 11 13
Watercontent(%)
AV1
AV2
AV3
K10
0
2
4
6
8
10
12
1 3 5 7 9 11 13
Titratableacidity
(mg/100g)
AV1
AV2
AV3
K10
20%
40%
60%
80%
100%
1 2 3 4 5 6 7
Watercontent(%)
Days of storage
AV1
AV2
AV3
K27 0
2
4
6
8
10
12
1 2 3 4 5 6 7
Titratableacidity
(mg/100g)
Days of storage
AV1
AV2
AV3
K27
	
Figure	2.	 Nutritional	(water	content	and	acidity)	characteristics	of	strawberry	fruit	during	
storage	 under	 different	 storage	 temperature	 conditions	 (4,	 10	 and	 27°C)	 and	
different	Aloe	vera	coating	treatments	(AV1,	AV2	and	AV3).	K	indicates	control	at	
specific	 temperature	 conditions	 without	 Aloe	vera	 coating	 treatments.	 The	 data	
are	an	average	from	5	sample	measurements.
670
Figure	3	shows	nutritional	characteristics	of	vitamin	C	content	from	strawberry	fruit	
during	 storage	 under	 different	 temperature	 conditions	 and	 different	 Aloe	 vera	 coating	
treatments.	Aloe	vera	coating	can	maintain	higher	vitamin	C	content	of	strawberry	fruit	at	
different	 temperature	 conditions	 compared	 with	 those	 without	 Aloe	vera	 coating.	 Coating	
strawberry	 fruit	 with	 Aloe	 vera	 at	 middle	 and	 lower	 temperatures	 will	 maintain	 higher	
vitamin	C	content	until	13	days	(AV1),	and	at	tropical	temperature	until	7	days	(AV3).	This	
indicates	 that	 Aloe	 vera	 coating	 treatment	 of	 strawberry	 fruit	 will	 have	 an	 effect	 on	
maintaining	 stability	 of	 vitamin	 C	 during	 storage	 although	 in	 tropical	 temperature	
conditions.	
	
Figure	3.	 Nutritional	 (vitamin	 C	 content	 and	 total	 soluble	 solid)	 characteristics	 of	
strawberry	 fruit	 during	 storage	 under	 different	 storage	 temperature	 conditions	
(4,	10	and	27°C)	and	different	Aloe	vera	coating	treatments	(AV1,	AV2	and	AV3).	K	
indicates	 control	 at	 specific	 temperature	 conditions	 without	 Aloe	 vera	 coating	
treatments.	The	data	are	an	average	from	5	sample	measurements.	
Figure	 3	 shows	 nutritional	 characteristics	 of	 total	 soluble	 solid	 content	 from	
strawberry	fruit	during	storage	under	different	temperature	conditions	and	different	Aloe	
vera	coating	treatments.	Aloe	vera	coating	can	maintain	higher	total	soluble	solid	content	of	
strawberry	fruit	at	different	temperature	conditions	compared	with	those	without	Aloe	vera	
coating.	This	indicates	that	Aloe	vera	coating	treatment	of	strawberry	fruit	will	have	an	effect	
on	maintaining	stability	of	soluble	solid	content	through	delayed	maturation	process	during
  671
ripening	because	it	covers	their	skin	with	an	Aloe	vera	coating.	This	condition	also	occurred	
in	strawberry	cultivar	‘Kamarosa’	(Zafari	et	al.,	2015).	
Based	on	the	data	mentioned	above,	it	can	be	summarized	that	Aloe	vera	coating	can	
maintain	 quality	 parameters	 of	 physical	 (weight	 loss	 and	 skin	 texture)	 and	 nutritional	
(titratable	acidity,	vitamin	C	and	total	soluble	solid	content)	better	than	those	without	Aloe	
vera	coating.	The	combination	of	Aloe	vera	coating	with	lower	temperature	on	strawberry	
fruit	can	extend	their	shelf	life.	Furthermore,	the	best	combination	between	the	treatments	
of	Aloe	vera	coating	and	temperature	conditions	are	40%	Aloe	vera,	40%	ethanol,	0.5%	CMC,	
2.5%	of	corn	syrup	and	17%	water	(AV3)	at	middle	controlled	temperature	(10°C	and	RH	
68%)	with	66%	maturity	level	of	fruit	strawberry.	
CONCLUSIONS	
The	 Aloe	vera	 coating	 can	 be	 used	 to	 maintain	 the	 quality	 and	 extend	 shelf-life	 of	
strawberry	 fruit	 in	 tropical	 temperature	 conditions.	 Furthermore,	 40%	 Aloe	 vera,	 40%	
ethanol,	 0.5%	 CMC,	 2.5%	 of	 corn	 syrup	 and	 17%	 water	 (AV3)	 at	 middle	 controlled	
temperature	(10°C	and	RH	68%)	with	66%	maturity	level	of	strawberry	fruit	are	the	best	
conditions	of	combination	between	Aloe	vera	coating	treatments	and	different	temperature	
conditions.	
ACKNOWLEDGEMENTS	
The	authors	want	to	thank	all	their	colleagues	and	students	for	their	assistance	in	the	
research	for	this	paper.	The	first	author	also	thanks	the	Research	Directorate	of	GadjahMada	
University	 and	 Ministry	 of	 Research	 and	 Technology	 and	 Higher	 Education,	 Republic	
Indonesia	for	their	grant:	78/LPPM/2015	and	No	025/	SP2H/PL/Dit.Litabmas/II/2015.	
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Quality and shelf-life improvement of fresh strawberry (Fragaria sp. ‘Holibrite’) using Aloe vera coating during storage in a tropical environment

  • 1.       Acta Hortic. 1194. ISHS 2018. DOI 10.17660/ActaHortic.2018.1194.95 Proc. VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits – Ethical and Technological Issues Eds.: F. Artés-Hernández et al. 665 Quality and shelf-life improvement of fresh strawberry (Fragaria sp. ‘Holibrite’) using Aloe vera coating during storage in a tropical environment M.A.F. Falaha, P. Yuliastuti, Jumeri and N. Khuriyati Faculty of Agricultural Technology, GadjahMada University, Yogyakarta, Indonesia. Abstract Fresh strawberry (Fragaria sp. ‘Holibrite’) sharply decrease their quality and also have a short shelf-life in a tropical environment conditions. The coating method using Aloe vera was applied to strawberry fruit to preserve their quality and increase their shelf life. Aloe vera is used as a biopolymer coating to cover strawberry fruit skin with standard formulation as gel coating. Different maturity levels of strawberry fruits were used and the quality parameters of fresh strawberry assayed were weight loss percentage, skin texture, water content, titratable acidity, vitamin C and total soluble solid content. Storage temperatures were set at 4°C with RH 62% and 10°C with RH 68% and then compared with the sample at room temperature as control for tropical environment. All quality parameters of strawberry fruit coated using Aloe vera could be kept stable at controlled temperature storage. Moreover, the shelf life of strawberry fruit under controlled storage temperatures was longer than those at tropical environment conditions. Aloe vera coating can preserve quality conditions and increase shelf life of strawberry fruit in a tropical controlled storage temperature and at room temperature. Keywords: Aloe vera coating, quality, strawberry, shelf-life, tropical environment INTRODUCTION Strawberry (Fragaria sp.) are a unique and popular fruit with highly desirable taste and flavour, visual appeal, excellent dietary sources of ascorbic acid, potassium, fibre, simple sugar as sources of energy and highly perishable products that lose sensory quality shortly after harvest, being susceptible to mechanical injury,water loss, decay and physiological deterioration which limits their shelf life to a few days at ambient temperature (Azodanlou, et al., 2003; Giampieri et al., 2012). Production of strawberry in Indonesia as a tropical country was increased 24% in 2011-2013 and they were cultivated in valley and mountain areas such as in Central Java (Purbalingga, Magelang and Semarang), in East Java (Malang, Bondowoso and Batu), in West Java (Ciwidey and Garut) and also Brastagi, North Sumatera (Anon., 2012; Hanif et al., 2011). Local strawberry in Indonesia from Ciwidey, Garut, West Java, were strongly recommended to store in lower temperature conditions, around 10°C, to maintain their quality (Falah et al., 2016). However, in a tropical environment, postharvest losses of horticultural products such as strawberry fruit is one of the main problems. This loss of quality and quantity of the horticultural product through their rapid deterioration occurs during handling, transportation and also storage. To overcome these problems several treatments were applied by the producer and fresh industrial user during their chain. Cooling treatment is one of the main treatments to preserve and maintain the quality and quality of the fruit, furthermore, edible coating is one of the alternative treatments that is applied nowadays. Application of edible coatings has been shown to be promising as a tool to improve the quality and extend storage as well as shelf life of various fruit such as papaya (Marpudi et al., 2011), table grapes (Valverde et al., 2005), tomato (Athmaselvi et al., 2013), sweet cherry (Martı́nez-Romero et al., 2006) and strawberries (Vahdat et al., 2010; Zafari et al., 2015; a E-mail: affan_tip@ugm.ac.id
  • 2. 666 Sogvar et al., 2016). Edible coatings have demonstrated the capability of improving food quality and prolonging shelf life of fresh produce through regulating the transfer of moisture, oxygen, carbon dioxide, aroma, and taste compounds in a food system (Castillo et al., 2010), and they can act as moisture and gas semi-permeable barriers, resulting in control of microbial growth, preservation of color and texture (Bourtoom, 2008). One of the novel edible coatings to maintain the quantity and quality of horticultural products is Aloe vera (Serrano et al., 2006; Valverde et al., 2005). Aloe vera has antifungal activity against several pathogenic fungi including Botrytis cinerea (De Rodrıguez et al., 2005). Aloe vera coatings modify the internal gas atmosphere, reduce moisture loss, softening, respiration rates, delay oxidative browning and reduce microorganism proliferation in fruit such as papaya, sweet cherries, table grapes, and also strawberries (Marpudi et al., 2011; Martı́nez-Romero et al., 2006; Valverde et al., 2005; Vahdat et al., 2010; Zafari et al., 2015; Sogvar et al., 2016). In this study, we investigated the effects of edible coating using Aloe vera treated in various temperature conditions to several quality parameters of fresh strawberry (weight loss percentage, skin texture, water content, titratable acidity, vitamin C and total soluble solid content) that were produced using soil cultivation in a tropical environment. MATERIALS AND METHODS Plant materials Strawberry (Fragaria sp. ‘Holibrite’) fruit were produced by conventional method using soil cultivation, located in Ketep Pass Banyuroto Village, Sawangan District, Magelang, Central Java, Indonesia. This farm is located between longitude 110°01’51” and 110°26’58”E and latitude between 7°19’13” and 7°42’16”S in the highland with 1200 m a.s.l., with climatic conditions in average air temperature 18-25°C and average relative humidity of 80- 90%. Strawberry fruit maturation had three different types, first type was full maturation with more than 80% red color of the skin strawberry with age of the fruit around 30 days after flowering (F), second type was 66% red color of the skin strawberry with age of the fruit around 25 days after flowering (F1) and third type was 33% red color of the skin of strawberry with age of the fruit around 20 days after flowering (F2). Edible coating formulation and environmental conditions Edible coating of Aloe vera had three different formulations, all formula used 0.5% carboxymethyl cellulose (CMC) and 2.5% of corn syrup that was added to each formula. The first formula had a composition of 25% Aloe vera, 25% ethanol, and 47% water (AV1), the second formula had a composition of 32.5% Aloe vera, 32.5% ethanol, and 32% water (AV2) and the third formula had a composition of 40% Aloe vera, 40% ethanol, and 17% water (AV3). During formulation of the edible coating solution, the solution was mixed and stirred with hot water at 80±2°C for 30 min, then fruit were dipped in solution of edible coating formula with immersion time of 3 min, drained by using perforated plastic containers, and air-dried over nylon screen for 90 min. Edible coating of strawberry fruit was then wrapped in three types of packaging, first unperforated packaging (UP), perforated packaging (P) and unperforated packaging with cardboard mat (UPC), same as the condition of strawberry in the market. Furthermore, these strawberry fruit with Aloe vera edible coating were stored in different conditions of temperature, ambient temperature (27°C), distribution centre temperature (10°C) and supermarket temperature (4°C). Strawberry fruits with edible coating were compared with those without coating at the same temperature conditions that were mentioned before. We measured physical and nutritional characteristics of these strawberries for several days until conditions of the strawberry fruit were not acceptable anymore to be consumed. Physical and nutritional quality evaluation of strawberry Quality evaluation of fresh strawberry fruit was done based on their physical and nutritional characteristics. For physical evaluation, weight loss percentage was measured using a balance (T and D, Co. Ltd., Osaka, Japan) where the formula is [(FW1–FW2)/FW2)
  • 3.   667 ×100%], FW1 is fresh weight at the first day measurement and FW2 is fresh weight at the second day of measurement. Texture of strawberry fruit skin as fruit firmness was determined using a texture analyzer (UTM, Model ZwickTipe DO FB0 5TS, Germany). Fresh weight measurements showed the average data from 1 packaged sample containing 18 strawberry fruit for samples and 5 samples of strawberry fruit with triplicates were used for texture measurement. The strawberry fruit were then evaluated until the strawberry fruits were not acceptable anymore to be eaten. For nutritional evaluation, water content was evaluated using a thermogravimetri method (Sato et al., 2006), soluble solid content (% Brix) was measured using a refractometer (PAL-1, Atago Co. Ltd., Japan), acidity of fruits was measured using the value of titratable acidity method and ascorbic acid or vitamin C was determined using the indophenols titration method. All nutritional measurements used a standard method (AOAC, 1990), and they were evaluated for 5 days and the average data from 5 samples for each measurement were used with triplicates. Data obtained from the experiment results were tabulated and calculated using Microsoft Excel 2007 (Microsoft Corporation). Further statistical analysis utilized SPSS version 13.0 (SPSS Incorporation) to obtain optimum treatments and significance of the obtained results through analysis of variance (ANOVA) and LSD interpretations. RESULTS AND DISCUSSION Harvested fresh strawberry fruit as a sample were transported around 1 h to the Faculty of Agricultural Technology Gadjahmada University Yogyakarta, Indonesia. The samples of strawberry fruit were then coated using Aloe vera with several treatments and different conditions of temperature during their storage. Physical and nutritional quality parameters of strawberry fruit coated with Aloe vera were evaluated based on the treatments and conditions mentioned above, and they were compared with control strawberry without coating. Physical characteristics of strawberry fruit quality Figure 1 shows physical characteristics of weight loss percentage from strawberry fruit during storage under different temperature conditions and different Aloe vera coating treatments and compared with control strawberry fruit at specific temperature conditions without Aloe vera coating treatments. At lowest temperature conditions (4°C), coating with Aloe vera had different patterns, where weight loss percentage of AV3 was higher than AVI, however, both of them showed losses in fresh weight faster than the control temperature. However, control without Aloe vera coating at the lowest temperature had the shortest shelf life. We supposed that the lowest temperature at 4°C can affect physiology and respiration of the strawberry fruit and coating with Aloe vera can maintain their quality from the unfavorable effect of the surroundings’ temperature, although chilling injury did occur. At the lowest temperature of the coating with Aloe vera, strawberry fruit can maintain their shelf life and are still acceptable to be eaten 10 days after storage. At the middle temperature conditions (10°C), fresh strawberry coated with Aloe vera (AV3) had the longest shelf life conditions (14 days). We suggest that at the middle temperature conditions Aloe vera coating can maintain their weight loss better without chilling injury. At this temperature, control of fresh strawberry without coating had a shorter shelf life compared to those with coating. Furthermore, Figure 1 also shows coating with Aloe vera in the tropical temperature conditions can reduce weight loss percentage and extend shelf life conditions. Figure 1 shows physical characteristics on change of texture of strawberry fruit skin during storage under different temperature conditions and different Aloe vera coating treatments and compared with control strawberry fruit at specific temperature conditions without Aloe vera coating treatments. The texture of the skin of fresh strawberry was relatively more firm in the lowest temperature compared with the middle temperature or tropical temperature. Coating with Aloe vera also slowly softened of the skin of fresh strawberry and increased shelf life of strawberry fruit compared to without coating at tropical room temperature. Firmness decreased relatively little at middle and lowest
  • 4. 668 temperature in strawberries uncoated and coated with Aloe vera. These conditions were also similar to those reported by Sogvar et al. (2016). Longest shelf life and stable texture of the skin of the strawberry occurred in the Aloe vera coating of AV3 treatments at the middle temperature (10°C). Decreasing skin texture or firmness and Aloe vera coating making the change of texture of the skin slower can be associated with the barrier of Aloe vera coating and the O2 uptake mechanism (Bhaskara Reddy et al., 2000). Aloe vera coating of fresh strawberry can maintain the physical quality of fresh strawberry at different conditions of temperature, specifically in tropical environment conditions without control, they can protect weight loss percentage, texture of the skin and then can maintain quality and increase shelf life. 0% 20% 40% 60% 80% 100% 1 2 3 4 5 6 7 8 9 10 Weight loss percentage(%) AV1 AV2 AV3 K4 0 2 4 6 8 1 2 3 4 5 6 7 8 9 1011121314Textureofskin(N) AV1 AV2 AV3 K4 0% 20% 40% 60% 80% 100% 1 2 3 4 5 6 7 8 9 10 Weight loss percentage(%) AV1 AV2 AV3 K10 0 2 4 6 8 1 2 3 4 5 6 7 8 9 1011121314 textureofskin(N) AV1 AV2 AV3 K10 0% 20% 40% 60% 80% 100% 1 2 3 4 5 Weightloss percentage(%) Days of storage AV1 AV2 AV3 K27 0 2 4 6 8 1 2 3 4 5 6 7 Textureoftheskin(N) Days of storage AV1 AV2 AV3 K27 Figure 1. Physical characteristics (weight loss percentage and texture of the skin) of strawberry fruit during storage under different storage temperature conditions (4, 10 and 27°C) and different Aloe vera coating treatments (AV1, AV2 and AV3). K indicates control at specific temperature conditions without Aloe vera coating treatments. The data are an average from 18 sample measuements of weight loss and 5 sample measurements of skin texture. Nutritional characteristics of strawberry fruit quality Figure 2 shows nutritional characteristics of water content from strawberry fruit during storage under different temperature conditions and different Aloe vera coating treatments then compared with control strawberry fruit at specific temperature conditions without Aloe vera coating treatments. Water content of strawberry fruit coated with Aloe vera was not significantly different with strawberry fruit without coating at the lowest and middle temperature conditions. However, water content of strawberry fruit in tropical conditions without Aloe vera coating showed a small decrease although not significantly
  • 5.   669 different. Figure 2 also shows nutritional characteristics of acidity from strawberry fruit during storage under different temperature conditions and different Aloe vera coating treatments. The acidity content of strawberry fruit was significantly different under different temperature conditions, where at higher temperature, their acidity content was lower compared with strawberries coated with Aloe vera. At middle and lowest temperature (10 and 4°C), acidity content of strawberry fruit without coating was relatively similar with those coated with Aloe vera. Coating with Aloe vera in combination with lower controlled temperature (10 and 4°C) can maintain strawberries’ acidity, and will extend shelf life of fresh strwberry fruit. The slow decline of acidity content with Aloe vera coating under low temperature might be supposed through metabolic changes in fruit resulting from organic acids used in the respiratory process (Echeverria and Valich, 1989) and the Aloe vera coating effect on acidity content of strawberry fruit could produce modification of internal atmosphere (Martı́nez-Romero et al., 2006) during their storage in combination with lower temperature storage. 20% 40% 60% 80% 100% 1 3 5 7 9 11 13 Watercontent(%) AV1 AV2 AV3 K4 0 2 4 6 8 10 12 1 3 5 7 9 11 13 Titratableacidity (mg/100g) AV1 AV2 AV3 K4 20% 40% 60% 80% 100% 1 3 5 7 9 11 13 Watercontent(%) AV1 AV2 AV3 K10 0 2 4 6 8 10 12 1 3 5 7 9 11 13 Titratableacidity (mg/100g) AV1 AV2 AV3 K10 20% 40% 60% 80% 100% 1 2 3 4 5 6 7 Watercontent(%) Days of storage AV1 AV2 AV3 K27 0 2 4 6 8 10 12 1 2 3 4 5 6 7 Titratableacidity (mg/100g) Days of storage AV1 AV2 AV3 K27 Figure 2. Nutritional (water content and acidity) characteristics of strawberry fruit during storage under different storage temperature conditions (4, 10 and 27°C) and different Aloe vera coating treatments (AV1, AV2 and AV3). K indicates control at specific temperature conditions without Aloe vera coating treatments. The data are an average from 5 sample measurements.
  • 6. 670 Figure 3 shows nutritional characteristics of vitamin C content from strawberry fruit during storage under different temperature conditions and different Aloe vera coating treatments. Aloe vera coating can maintain higher vitamin C content of strawberry fruit at different temperature conditions compared with those without Aloe vera coating. Coating strawberry fruit with Aloe vera at middle and lower temperatures will maintain higher vitamin C content until 13 days (AV1), and at tropical temperature until 7 days (AV3). This indicates that Aloe vera coating treatment of strawberry fruit will have an effect on maintaining stability of vitamin C during storage although in tropical temperature conditions. Figure 3. Nutritional (vitamin C content and total soluble solid) characteristics of strawberry fruit during storage under different storage temperature conditions (4, 10 and 27°C) and different Aloe vera coating treatments (AV1, AV2 and AV3). K indicates control at specific temperature conditions without Aloe vera coating treatments. The data are an average from 5 sample measurements. Figure 3 shows nutritional characteristics of total soluble solid content from strawberry fruit during storage under different temperature conditions and different Aloe vera coating treatments. Aloe vera coating can maintain higher total soluble solid content of strawberry fruit at different temperature conditions compared with those without Aloe vera coating. This indicates that Aloe vera coating treatment of strawberry fruit will have an effect on maintaining stability of soluble solid content through delayed maturation process during
  • 7.   671 ripening because it covers their skin with an Aloe vera coating. This condition also occurred in strawberry cultivar ‘Kamarosa’ (Zafari et al., 2015). Based on the data mentioned above, it can be summarized that Aloe vera coating can maintain quality parameters of physical (weight loss and skin texture) and nutritional (titratable acidity, vitamin C and total soluble solid content) better than those without Aloe vera coating. The combination of Aloe vera coating with lower temperature on strawberry fruit can extend their shelf life. Furthermore, the best combination between the treatments of Aloe vera coating and temperature conditions are 40% Aloe vera, 40% ethanol, 0.5% CMC, 2.5% of corn syrup and 17% water (AV3) at middle controlled temperature (10°C and RH 68%) with 66% maturity level of fruit strawberry. CONCLUSIONS The Aloe vera coating can be used to maintain the quality and extend shelf-life of strawberry fruit in tropical temperature conditions. Furthermore, 40% Aloe vera, 40% ethanol, 0.5% CMC, 2.5% of corn syrup and 17% water (AV3) at middle controlled temperature (10°C and RH 68%) with 66% maturity level of strawberry fruit are the best conditions of combination between Aloe vera coating treatments and different temperature conditions. ACKNOWLEDGEMENTS The authors want to thank all their colleagues and students for their assistance in the research for this paper. The first author also thanks the Research Directorate of GadjahMada University and Ministry of Research and Technology and Higher Education, Republic Indonesia for their grant: 78/LPPM/2015 and No 025/ SP2H/PL/Dit.Litabmas/II/2015. Literature cited Anon. (2012). Ministry of Agriculture: Strawberry Cultivation (in Indonesian) (Jakarta: Centre of Research and Development for Agriculture, Ministry of Agriculture). AOAC. (1990). Official Methods of Analysis, 15th edn (Arlington, VA: Association of Official Analytical Chemists). Athmaselvi, K.A., Sumitha, P., and Revathy, B. (2013). Development of Aloe vera based edible coating for tomato. Int. Agrophys. 27 (4), 369–375 https://doi.org/10.2478/intag-2013-0006. Azodanlou, R., Darbellay, C., Luisier, J.L., Villettaz, J.C., and Amadò, R. (2003). Quality assessment of strawberries (Fragaria species). J. Agric. Food Chem. 51 (3), 715–721 https://doi.org/10.1021/jf0200467. PubMed Bhaskara Reddy, M.V., Belkacemi, K., Corcuff, R., Castaigne, F., and Arul, J. (2000). Effect of pre-harvest chitosan sprays on post-harvest infection by Botrytis cinerea quality of strawberry fruit. Postharvest Biol. Technol. 20 (1), 39–51 https://doi.org/10.1016/S0925-5214(00)00108-3. Bourtoom, T. (2008). Edible films and coatings: characteristics and properties. Int. Food Res. J. 15, 237–248. Castillo, S., Navarro, D., Zapata, P.J., Guillén, F., Valero, D., Serrano, M., and Martı́nez-Romero, D. (2010). Antifungal efficacy of Aloe vera in vitro and its use as a preharvest treatment to maintain postharvest table grape quality. Postharvest Biol. Technol. 57 (3), 183–188 https://doi.org/10.1016/j.postharvbio.2010.04.006. De Rodrıguez, D.J., Hernández-Castillo, D., Rodrıguez-Garcıa, R., and Angulo-Sánchez, J.L. (2005). Antifungal activity in vitro of aloe vera pulp and liquid fraction against plant pathogenic fungi. Ind. Crops Prod. 21 (1), 81–87 https://doi.org/10.1016/j.indcrop.2004.01.002. Echeverria, E., and Valich, J. (1989). Enzymes of sugar and acid metabolism in stored ‘valencia’ oranges. J. Am. Soc. Hortic. Sci. 114, 445–449. Falah, M.A.F., Hurrin Husna, A.R.P. and Andam Dewi Jumeri. (2016). Quality evaluation of fresh strawberry (Fragaria sp. cv. Earlibrite) during storage in a tropical environment. Paper presented at: Int. Seminar of ICST- 2015. Giampieri, F., Tulipani, S., Alvarez-Suarez, J.M., Quiles, J.L., Mezzetti, B., and Battino, M. (2012). The strawberry: composition, nutritional quality, and impact on human health. Nutrition 28 (1), 9–19 https://doi.org/10.1016/j. nut.2011.08.009. PubMed Hanif, Z., Emi Budiyati, and Oka Ardiana Banaty. (2011). Produktivitas Sepuluh Aksesi stroberi yang Berkembang di lndonesia. Paper presented at: National Seminar on Pemberdayaan petani melalui inovasi teknologi spesifik lokasi (In Indonesian). Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian (Yogyakarta).
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